102,515 research outputs found
Per una rilettura del Vaso Alfonsi: dall'immagine al territorio
No full textIl contributo prende in esame un famoso vaso con decorazione figurata rinvenuto nel 1907 e più volte edito, ma mai con una analisi di dettaglio dell'iconografia.
Si propone una lettura completamente nuova , non solo iconografica ma iconologica, che porta ad una migliore comprensione della relazione tra il vaso, la decorazione e il territorio di cui è espressione, fino ad una più mirata interpretazione della sua funzione nel contesto funerario in cui è stato rinvenuto
Fermi LAT View of a Sample of Flaring gamma-Ray AGNs
No full textIn the first 3.5 years of operations, Fermi detected several sources whose flaring activity brought them to exceed daily fluxes brighter than F(E > 100MeV) > 10(-6) ph cm(-2) s(-1). These episodes were promptly reported to the scientific community by the Fermi collaboration by means of astronomer telegrams (ATels). We focus our attention on the sample composed by these flaring sources, most of which are blazars, known to be extremely variable over the whole electromagnetic spectrum, from radio to gamma-ray energies. We study properties of the selected sample and compare them to general characteristics of the Fermi source catalogue
Primary cell cultures from fetal bovine hypothalamus and cerebral cortex: A reliable model to study P450Arom and alpha and beta estrogen receptors in vitro.
No full textEstrogens synthesized by neural P450 aromatase (P450Arom) are implicated in many aspects of mammalian brain development and particularly in sexual differentiation of the central nervous system (CNS). This study analyzes the usefulness of an in vitro model based on bovine primary cell cultures from the hypothalamus and frontal cortex to investigate the role of P450Arom and estrogen receptors (ERs) in the development of fetal neural structures. The mRNA expression of P450Arom, ERα and ERβ was detected using RT-PCR analysis in both hypothalamic and cortical primary cell cultures. P450Arom was identified and localized by immunocytochemistry in both neurons and astrocytes. Our results indicate that, within our experimental settings, astrocytes do not express ERα. The experimental model that we propose may represent a standardized dynamic model to study cellular and molecular mechanisms involved in the complex process of brain sexual differentiation
Primary cell cultures from fetal bovine brain: an in vitro model to study neuroactive steroids
No full textEffects of Duplex-specific nuclease on human cells expression profiling using RNA-seq
No full textEffects of Duplex-specific nuclease on human cells expression profiling using RNA-seqV. Mijatovic 1 , G. Buson 2 , M. Valenti 3 , L. Dalle Carbonare 3 , A. Ferrarini 2 , A. Dal Molin 1 , A. Mori 1 , L. Xumerle 1 , E. Caviola 4 , P. Pertile 4 , M. Delledonne 2 , P. F. Pignatti 1 , G. Malerba 1 ;1 Department of Life and Reproductions Sciences, University of Verona, Verona, Italy, 2 Department of Biotechnology, University of Verona, Verona, Italy, 3 Department of Medicine, Clinic of Internal Medicine, Section D, Verona, Italy, 4 Cutech Srl, Padova, Italy.RNA-seq is a next-generation sequencing method able to characterize tho-ence the estimate of relative expression of less expressed genes, as well as reduce the estimate accuracy of gene expression levels of low expressed genes. Therefore, it would be convenient to apply a method able to reduce excessively abundant mRNA transcripts. A cDNA depletion method for the most represented transcripts called duplex-specific nuclease (DSN) has been effectively used in several studies. Three different cell types were studied: blood, monocytes and keratinocytes. From each cell type two RNA-seq libraries were produced (untreated libraries), for a total of 6 libraries. A part of each library was then treated with DSN, producing a total of 6 DSN treated libraries. Overall, 12 RNA-seq libraries (6 untreated and 6 treated) were prepared. In blood tissue the most abundant transcripts in both DSN-treated and DSN-untreated samples corresponded to globins (HBA2, HBA1 and HBB), accounting for ~70% of total transcripts. In this sample maximum effects of DSN treatment was observed. Transcripts found to be the most expressed in monocyte sample were ~10 times smaller than globins expression in blood sample. Gene expression of keratinocyte libraries was not influenced by DSN treatment. DSN treatment has been able to strongly reduce globins expression. DSN treatment also reduced, even if with a smaller effect, the most expressed genes in monocyte sample
FERMI LAT view of a sample of flaring gamma-ray AGN
No full textRadio-loud AGNs, especially blazars, are sources known to be extremely variable over the whole electromagnetic spectrum, from radio to γ-ray energies. Up to now, the Large Area Telescope (LAT), the primary instrument on board the Fermi satellite, has detected more than 850 sources that could be associated with AGNs. Several of them underwent at least one flaring episode at γ-rays during the time spanned by the Fermi observations. We present and discuss a list of flaring AGNs detected during the first 3.5 years of Fermi operations with fluxes brighter than F(E > 100 MeV) > 10−6 ph cm −2 s −1, the threshold set by the LAT Collaboration for issuing an Astronomer Telegram. More, the general characteristics of the selected sample are investigated
Association of age-related macular degeneration with polymorphisms in vascular endothelial growth factor and its receptor.
No full textOBJECTIVE:
To analyze the association between age-related macular degeneration (AMD) and polymorphisms in vascular endothelial growth factor (VEGF) and VEGF receptor KDR gene polymorphisms. A complex, multifactorial disease in which genetic and environmental factors interact, AMD is the leading cause of blindness in the elderly. Vascular endothelial growth factor (VEGF), a key regulator of angiogenesis, is considered an important factor for the pathogenetic processes of AMD. Previous studies investigated the possible association between VEGF-A gene polymorphisms and AMD, with contrasting data. No study examined the possible role of VEGF receptor KDR gene polymorphisms.
DESIGN:
Case-control study.
PARTICIPANTS AND CONTROLS:
We enrolled 226 AMD cases and 248 controls from an ophthalmology hospital center.
METHODS:
Genotypying for 16 polymorphic markers (single nucleotide polymorphisms [SNPs]) in VEGF-A and KDR genes.
MAIN OUTCOME MEASURES:
Distribution of genotypes in AMD cases and controls.
RESULTS:
Two polymorphisms (rs833069 in intron 2 of the VEGF-A gene, rs2071559 in the promoter of the KDR gene) were significantly associated with risk of AMD. In particular, for VEGF-A rs833069 the AMD risk was increased >5-fold for G homozygotes compared with homozygous carriage of the A allele. For KDR rs2071559 the AMD risk was increased >3-fold for T homozygotes compared with homozygous carriage of the C allele. Carriers of risk alleles for both markers have a >6-fold increased risk of AMD with respect to carriers of non-risk alleles.
CONCLUSIONS:
We expand previous data on the association of AMD with VEGF-A gene variations and identify for the first time an association with variations in the KDR gene. Because the SNP-604T-bearing KDR promoter has higher transcription activity, our findings further support the role of the VEGF pathway in the pathophysiology of AMD. It is possible that applications of haplotype/genotype analysis in these genes will play a role in risk assessment and pharmacogenomic approaches to AMD diagnosis and management
Bibliographie Hilarion G. Petzold 1958 – 2009 mit Anhang als Einführung
No full textDieses Archiv enthält die Gesamtbibliographie der Werke des Autors nebst einiger Texte „Über H. G. Petzold“ im Schlussteil der Bibliographie sowie einen Anhang mit einer Einführung in die Architektur des Werkes in seinem wissenslogischen Aufbau als Ausarbeitung seines „Tree of Science Modells“ (2007).This archive contains the complete bibliography of the author and some texts about H. G. Petzold, moreover an epilogue with an introduction to the architecture of the works in its epistemological structure and composition and as an elaborations of Petzold’s „Tree of Science Modell (2007).https://www.fpi-publikation.de/polyloge/01-2009-petzold-h-g-gesamtbibliographie-h-g-petzold-1958-2009-updating-november2009/peerReviewedpublishedVersio
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
UB CCD Photometry of the Old, Metal-rich, Open Clusters NGC 6791, NGC 6819, and NGC 7142
No full textWe report on a UV-oriented imaging survey in the fields of the old, metal-rich open clusters NGC 6791, NGC 6819, and NGC 7142. With their super-solar metallicity and ages >~ 3-8 Gyr, these three clusters represent both very near and ideal stellar aggregates to match the distinctive properties of the evolved stellar populations, as in elliptical galaxies and bulges of spirals. Following a first discussion of NGC 6791 observations in an accompanying paper, here we complete our analysis, also presenting for NGC 6819 and NGC 7142 the first-ever U CCD photometry. The color-magnitude diagram of the three clusters is analyzed in detail, with special emphasis on the hot stellar component. We report, in this regard, one new extreme horizontal-branch star candidate in NGC 6791. For NGC 6819 and 7142, the stellar luminosity function clearly points to a looser radial distribution of faint lower main sequence stars, either as a consequence of cluster dynamical interaction with the Galaxy or as an effect of an increasing fraction of binary stars toward the cluster core, as also observed in NGC 6791. Compared to a reference theoretical model for the Galaxy disk, the analysis of the stellar field along the line of sight of each cluster indicates that a more centrally concentrated thick disk, on a scale length shorter than ~2.8 kpc, might better reconcile the lower observed fraction of bright field stars and their white-dwarf progeny
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