1,720,961 research outputs found
Enzyme-linked immunosorbent assay for serodiagnosis of Mycoplasma pneumoniae infections.
No full textResearch into the presence of mycoplasma in some obstetric and cynaecological conditions
No full textInfluence of yeasts and of their constituents on nucleoside uptake in peritoneal murine macrophages
No full textA marked reduction of [3H]-uridine uptake was observed when mouse peritoneal macrophages (pM phi) were exposed to heat-killed Candida albicans or Saccharomyces cerevisiae. By contrast, an increased nucleoside uptake was promoted by yeast products such as zymosan, laminarin, or yeast cell-wall extracts, which are mainly composed of beta-glucans and alpha-mannans. In a search for the active fungal component(s), the uptake process was shown to be differently affected by monosaccharides and polysaccharides. These findings support the view that a specific recognition of a pM phi membrane receptor is mediating the effect of the various substances
Nucleoside uptake in macrophages from various murine strains: a short-time and a two-step stimulation model
No full textKinetics of [3H]-uridine uptake by murine peritoneal macrophages (pM phi) is early altered after exposure to a variety of stimuli. Alterations caused by Candida albicans, lipopolysaccharide (LPS) and recombinant interferon-gamma (rIFN-gamma) were similar in SAVO, C57BL/6, C3H/HeN and C3H/HeJ mice, and were not correlated with an activation process as shown by the amount of tumor necrosis factor-alpha (TNF-alpha) being released. Short-time exposure to all stimuli resulted in an increased nucleoside uptake by SAVO pM phi, suggesting that the tumoricidal function of this cell either depends from the type of stimulus or the time when the specific interaction with the cell receptor is taking place. Experiments with priming and triggering signals confirmed the above findings, indicating that the increase or the decrease of nucleoside uptake into the cell depends essentially on the chemical nature of the priming stimulus. The triggering stimulus, on the other hand, is only able to amplify the primary response
A new ELISA method for the detection of serum bindable anti-platelet antibodies (SPBIG).
No full textA microplate enzyme-linked immunospecific assay (ELISA) detecting unbound anti-platelet antibodies.
No full textA microplate immune-enzymatic method was developed for detecting serum antiplatelet antibodies. The method involves the use of antigen-coated platelets and alkaline phosphatase-labeled antihuman immunoglobulin. Linear correlation was obtained between the titer of platelet antibodies and substrate conversion. Twenty-four patients with immune thrombocytopenia and 40 normal controls were studied. Eighteen patients and one control were positive. Therefore, sensitivity and specificity were 75% and 97% respectively. ELISA also was found to be more sensitive than the indirect antiglobulin consumption assay (ACA) and appears to be a practical and easy method for routine evaluation of antiplatelet antibodies
Time-resolved EPR investigation of [70]fulleropyrrolidine nitroxide isomers
No full textA novel [70]fulleropyrrolidine functionalized with a nitroxide radical has been synthesized. After
pulsed photoexcitation, time-resolved electron paramagnetic resonance (EPR) spectra have been
recorded in liquid solution at standard X-band (9.5 GHz) and W-band (95 GHz) microwave
frequencies. The spectra exhibit strongly electron spin polarized ground and excited states, the
latter being arising from the spin coupling of the nitroxide’s electron spin with the fullerene’s
excited triplet state. The EPR parameters such as g-values and hyperfine coupling constants have
been discussed in terms of the spin Hamiltonian for excited doublet, triplet and quartet states. On
the basis of the strength of the exchange interaction between the fullerene triplet and the radical
group, two, out of a possible four [70]fulleropyrrolidine isomeric monoadducts, have been
characterized
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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