1,721,066 research outputs found
DQ386898, DQ386913,DQ418772,DQ418773, sequenze relative a marcatori molecolari SSR per la specie Olea europea L..
No full textDevelopment of SCAR markers for germplasm characterization in olive tree (Olea europea L.)
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Overview on molecular studies in olive (Olea europaea L.) : DNA markers application and first results in genome analysis.
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Storage-time effect on olive oil DNA assessed by Amplified Fragments Length Polymorphisms.
No full textMolecular Markers Analysis of the Genetic Variability inside the Species Crocus sativus L.
No full textThe real level of genetic variability inside the species Crocus sativus L. is still debated and in literature it is possible to recover contradictory papers providing contrasting results. If the species is monomorphic or polymorphic is under debate.
In order to obtain new insights about the relations between phenotypic and genetic variations between different accessions inside the species C. sativus L. we carried out a molecular marker analysis by using the AFLP (Amplified Fragment Length Polymorphism) and the MS-AFLP (Methyl Sensitive AFLP, susceptible to the different methylation state of cytosines) methodologies.
The use of these two methodologies gives us the possibility to detect the presence of variability both at the genomic and epigenomic level.
In a previous communication we have reported about the application of such methodologies on a core collection made of samples belonging both to accessions with different origins inside the species C. sativus and to different species of the Crocus genus. Variable levels of variation among the samples have been detected by the two methodologies.
Here we present a further advance by applying the two molecular methodologies abovementioned to a huge number of samples collected from the CrocusBank Germplasm located in Cuenca (Spain) funded by the European Commission AGRI GEN RES 018 Action. The Crocus Bank is a big collection of several accessions of saffron from different countries and regions, in order to characterise and exploit the genetic variability of C. sativus (www.crocusbank.org).
73 different saffron accessions originated from different Spanish cultivation areas have been considered to obtain a deeper evaluation of the genetic variability. Moreover in order to see if a certain level of genetic variability is also present inside the same accession, a number of samples ranging between 4 and 5 have been considered for all the collected samples. 10 selective primer combinations have been considered for both the two analyses and the preliminary results are reported
Ancient Pomoideae (Malus domestica Borkh. and Pyrus communis L.) cultivars in Appennino Toscano (Tuscany, Italy): molecular (SSR) and morphological characterization
No full textBacterial ecology of PDO Coppa and Pancetta Piacentina at the end of ripening and after MAP storage of sliced product.
No full textThe objective of this study was to evaluate the microbiota of two typical Italian PDO delicatessens Coppa and
Pancetta Piacentina, produced in Piacenza area (Italy). Classical and molecular approaches were employed, in
order to acquire knowledge on their bacterial ecology and its evolution after slicing and MAP storing; thus, the
biodiversity of characteristic bacterial community, already present or introduced during such procedures, was
studied in both full ripened and sliced samples from two producers (A and B) of the PDO district, packaged
under MAP and stored at 2 and 8 °C for 30 days.
The microbiota of the two kinds of Italian delicatessen demonstrated peculiar differences, particularly regarding
the staphylococci and lactic acid bacteria (LAB) ratio. Moreover, some species within these two groups appeared
to be linked to the kind of product: Leuconostoc, Lactobacillus versmoldensis and Staphylococcus saprophyticus
were found only in Pancetta while Lactobacillus pentosus, Staphylococcus equorum, Staphylococcus xylosus,
Staphylococcus sciuri andMacrococcus caseolyticus occurred only in Coppa. Also, both delicatessens fromproducer
A were richer in LAB compared to those of producer B and the opposite applied for staphylococci. Interestingly,
Tetragenococcus halophilus was detectable in all the samples and its presence in the sausage environment has
been reported only for Capocollo. Storage did not substantially modify the microbiota composition, the only
changes being the relative abundance of same sequences; S. xylosus was prevalent before slicing process and
S. equorum at the end of MAP storage at both 2 °C and 8 °C.
Concerning microbial contamination during the slicing process, our results suggest that the adopted procedures
assure high hygienic quality standard of these typical products, with exception of a contamination by
Psychrobacter psychrophilus in Coppa B.
The possible origin of species rarely or never reported in the sausage environment and detected in this study is discussed
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