1,721,080 research outputs found
Flow cytometry application in hematological malignancies of childhood
Full text linkThe PhD research work was performed, for the first part (1 year) at the Pediatric Haematology-Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia University and for the second part (2 years) at the Pediatric Haematology-Oncology Department, Padova University, two excellent setting for a specialized training in pediatric haematology-oncology. The PhD program was targeted in both a clinical and laboratory research experience in order to perform a translational research on pediatric patients affected by a wide range of hematological disorders, both malignant and non-malignant. The efforts were coordinated to study the biology and therapy of pediatric Acute Lymphoblastic Leukemia (ALL), Acute Myeloid Leukemia (AML) and Myelodysplastic Syndromes (MDS)
Moving forward in target antigen discovery for immunotherapy in acute myeloid leukemia
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Leukemia blast cell identification
No full textAcute leukemia is a heterogeneous group of disorders, characterized by different laboratory and prognostic features. An adequate diagnosis of acute leukemia, based on the identification of a leukemic cell population and the hematopoietic lineage assessment, is the first goal toward defining correct risk stratification of patients and tailoring specific therapies to patients. Diagnosis of acute leukemia underwent an important change since the 1970s when the only available diagnostic tools were cytomorphology and cytochemistry. Nowadays, the development of modern techniques and their combined use in a multimodal approach lead to a better identification and sub-classification of acute leukemia. Thus, blast identification in acute leukemia required a comprehensive global approach, by combining cytomorphology, cytochemistry, multiparameter flow cytometry (MFC), cytogenetic, fluorescence in situ hybridization (FISH) and molecular genetic methods. At least, new sequencing technologies, such as gene expression profiling (GEP) and whole-genome sequencing, DNA methylation arrays, and comparative genomic hybridization array, could represent the new frontiers in the characterization of genetic heterogeneity in acute leukemia. WIREs Data Mining Knowl Discov 2015, 5:74-85. doi: 10.1002/widm.1146 For further resources related to this article, please visit the
IDENTIFICATION OF IMMUNOPHENOTYPIC SIGNATURES BY CLUSTERING ANALYSIS IN PEDIATRIC PATIENTS WITH PHILADELPHIA CHROMOSOME-POSITIVE ACUTE LYMPHOBLASTIC LEUKEMIA
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New methodologic approaches for immunophenotyping acute leukemias
No full textBACKGROUND AND OBJECTIVES:
Flow cytometry is nowadays the preferred method for immunophenotypic identification, enumeration and characterization of blast cells at diagnosis. Despite widespread application of standardized protocols, inter-laboratory reproducibility has still not been achieved. The complexity of diagnosis and evaluation of minimal residual disease, in immunophenotyping acute leukemia, demands the use of a test that provides all the necessary information.
DATA SOURCES AND METHODS:
The information given here is derived from the experience of the authors and from literature files. The most relevant studies with adequate conclusions were considered. We report on the current status of multiparametric immunophenotyping using simultaneous three and four-color staining and the applications of this technique.
RESULTS:
Multiparametric immunophenotyping is a powerful method for achieving a clear discrimination between normal and pathologic cells. The specific identification of leukemic cells by immunologic gating forms the basis for immunophenotypic diagnosis, classification as well as prognostic evaluation of patients with acute leukemias. The performance of the procedure with regards to the panels of reagents and the analytic processes, is necessarily different in lymphoblastic and myeloblastic leukemias, since the diagnostic questions are different. Phenotypic information should be specifically provided for the blast cells and antigen expression should preferably be reported in quantitative units and CV. This would allow a standardized cross evaluation of immunophenotypic results between different investigators and laboratories.
INTERPRETATION AND CONCLUSIONS:
Recent reports indicate that phenotypic aberrations reflect genetic abnormalities of leukemic cells and therefore their definition and identification is of clinical relevance not only for minimal residual disease monitoring but also for subclassifying acute myeloid and lymphocytic leukemias
La sindrome di Dandy Walker: follow-up in due differenti casistiche
No full textNeurologia Pediatrica 1/2, 108-109 (42)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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