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Identifizierung und Charakterisierung von ERF1b-Transkriptionsfaktor- Bindungsmotiven und ihren Zielgenen
No full textThe Ethylene Response Factor (ERF) gene family is a subset of AP2
(Apetala2)/ERF superfamily. It is characterized by exhibiting an AP2/ERF
domain of 60 to 70 amino acids involved in DNA binding. In Arabidopsis
thaliana ERF1b transcription factor family contains eight members (Nakano et
al. 2006). All proteins in the group are characterized by a conserved AP2
domain, but non-conserved C- and N-termini. To investigate the functional
redundancy or specificity of the transcription factors, the binding sites of
Rap2.4a and Rap2.4d were defined. A new method, called stringent inverse
yeast-one-hybrid, was developed to screen full genomes for transcription
factor binding motifs and to identify transcription factor target genes. It
allowed efficient, fast and easy screening of the A. thaliana genome,
including the organellar genomes, for binding sites of Rap2.4a and Rap2.4d. Of
the Rap2.4a and Rap2.4d binding sites detected on the nuclear genome, 88 % and
94.7 %, respectively, were found in the promoter regions of genes,
demonstrating a high specificity of the screen for regulatory elements. The
transcript dynamics of Rap2.4d and Rap2.4c were shown to follow similar
patterns in response to cold other abiotic stresses, suggesting that the two
genes are redundant in function. Expression analysis of cold regulated genes
(COR6.6, COR47, COR15A, PLD1) in a Rap2.4c overexpression line showed that
Rap2.4c is a general negative regulator of cold responsive genes. Transient
reporter gene activity analysis demonstrated that Rap2.4c exerts its
regulatory function on the promoter region of cold responsive genes. Yeast-
one-hybrid assay and analysis of T-DNA insertion lines showed that Rap2.4a and
Rap2.4h regulate the expression of 2CPA antagonistically, possibly by
competing for the same binding site. However, Rap2.4h is a stronger competitor
than Rap2.4a as demonstrated by vigorous binding of 2CPA promoter. Promoter
analysis using the identified motifs, yeast-one-hybrid assay and expression
analysis of T-DNA insertion lines indicated that Rap2.4a and Rap2.4d
positively regulate expression sAPx and tAPx.Die Ethylene Response Faktoren (ERF) Gene gehören zur AP2 (Apetala2)/ERF
Superfamilie. Diese ist durch eine AP2/ERF DNA Bindedomäne charakterisiert,
die aus 60-70 Aminosäuren besteht. Die Familie der ERFIb
Transkriptionsfaktoren beinhaltet 8 Mitglieder (Nakano et al. 2006) mit
konservierter AP2 Domäne und nicht-konservierte C- und N-Termini. Um eine
mögliche funktionelle Redundanz von der Spezifität der Transkriptionsfaktoren
abzugrenzen, wurden die Bindemotive der Faktoren Rap2.4a und Rap2.4d
identifiziert. Hierfür wurde eine neue Methode, das sogenannte „Stringente
inverse Hefe-1-Hybrid-System“, entwickelt. Es ermöglicht eine genomweite
Überprüfung von Transkriptionsfaktor-Bindestellen und zeichnet sich durch eine
einfache und effiziente Handhabung aus. In der vorliegenden Arbeit wurde es
zur Identifizierung von Rap2.4a-und Rap2.4d-Bindestellen in Arabidopsis
thaliana, einschließlich der Organellgenome, eingesetzt. 88 % bzw. 94.7 % der
in genomischer DNA identifizierten Rap2.4a-und Rap2.4d-Bindemotive liegen in
Promotorabschnitten. Dies bestätigte die hohe Spezifität der Methode. Die
Transkriptspiegel von Rap2.4d und Rap2.4c werden durch Kälte und anderen
abiotischen Stressformen ähnlich reguliert, was für eine funktionelle
Redundanz der Gene spricht. Expressionsanalysen zu den kälteregulierten Genen
COR6.6, COR47, COR15A und PLD1 in Rap2.4c-Überexpressionlinien zeigten, dass
der Transkriptionsfaktor einen generellen, reprimierenden Einfluss auf
kälteregulierte Gene hat. Hefe-1-Hybrid Resultate sowie T-DNA
Insertionsanalysen zeigten, dass Rap2.4a und Rap2.4h die Expression von 2CPA
antagonistisch regulieren. Möglich wäre eine Konkurrenz um die gleiche
Bindestelle auf dem 2CPA-Promotor, wobei Rap2.4h eine stärkere Bindeaffinität
hat als Rap2.4a. Promotoranalysen, Hefe-1-Hybrid Experimente und
Expressionsanalysen lassen darauf schließen, dass Rap2.4a und Rap2.4d die
Expression von sAPx und tAPx positiv regulieren
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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