327 research outputs found
analysis of heterologous protein production
No full textThe methylotrophic yeast Pichia pastoris has gained much attention during the last decade as a platform for producing heterologous recombinant proteins of pharmaceutical importance, due to its ability to reproduce post-translational modification similar to higher eukaryotes. With the recent release of the full genome sequence for P. pastoris, in-depth study of its functions has become feasible. Here we present the first reconstruction of the genome-scale metabolic model of the eukaryote P. pastoris type strain DSMZ 70382, PpaMBEL1254, consisting of 1254 metabolic reactions and 1147 metabolites compartmentalized into eight different regions to represent organelles. Additionally, equations describing the production of two heterologous proteins, human serum albumin and human superoxide dismutase, were incorporated. The protein-producing model versions of PpaMBEL1254 were then analyzed to examine the impact on oxygen limitation on protein production.This work was supported by the Korean Systems Biology
Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST)
through the National Research Foundation of Korea,
by the Austrian Science Fund (FWF), project I37-
B03, and the Austrian Research Promotion Agency
(program FHplus)
The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris
No full textThe methylotrophic yeast Pichia pastoris is a popular yeast expression system for the production of heterologous proteins in biotechnology. Interestingly, cell organelles which play an important role in this process have so far been insufficiently investigated. For this reason, we started a systematic approach to isolate and characterize organelles from P. pastoris. In this study, we present a procedure to isolate microsomal membranes at high purity. These samples represent endoplasmic reticulum (ER) fractions which were subjected to molecular analysis of lipids and proteins. Organelle lipidomics included a detailed analysis of glycerophospholipids, fatty acids, sterols and sphingolipids. The microsomal proteome analyzed by mass spectrometry identified typical proteins of the ER known from other cell types, especially Saccharomyces cerevisiae, but also a number of unassigned gene products. The lipidome and proteome analysis of P. pastoris microsomes are prerequisite for a better understanding of functions of this organelle and for modifying this compartment for biotechnological applications. (C) 2013 Elsevier B.V. All rights reserved
Digital humanities and digital social reading
No full textRebora S, Boot P, Pianzola F, et al. Digital humanities and digital social reading. Digital Scholarship in the Humanities. 2021;36(Supplement_2):ii230-ii250.Prominent among the social developments that the web 2.0 has facilitated is digital social reading (DSR): on many platforms there are functionalities for creating book reviews, ‘inline’ commenting on book texts, online story writing (often in the form of fanfiction), informal book discussions, book vlogs, and more. In this article, we argue that DSR offers unique possibilities for research into literature, reading, the impact of reading and literary communication. We also claim that in this context computational tools are especially relevant, making DSR a field particularly suitable for the application of Digital Humanities methods. We draw up an initial categorization of research aspects of DSR and briefly examine literature for each category. We distinguish between studies on DSR that use it as a lens to study wider processes of literary exchange as opposed to studies for which the DSR culture is a phenomenon interesting in its own right. Via seven examples of DSR research, we discuss the chosen approaches and their connection to research questions in literary studies
Ich weiss was passiert – Freunde und Medienfiguren verstehen. Eine Untersuchung zur Empathie bei Kindern in realen und fiktionalen Welten
No full textIn der Dissertation werden Zusammenhänge zwischen der Empathie im Alltag und der Empathie zu Medienfiguren aufgrund von Leitfadengesprächen mit 12-13jährigen Kindern untersucht
Les relations entre l'architecture gothique eu Suisse romande et en Bourgogne
No full textDie Beziehungen zwischen der gotischen Architektur in der Westschweiz und in Burgund, von Stephan Gasser
Eine vor kurzem vom Autor dieses Artikels abgeschlossene Untersuchung zur gotischen Architektur in der Westschweiz hat gezeigt, dass die mimer wieder propagierten Verbindungen zur Architektur im Burgund und in der Freigrafschah relativiert werden müssen. Demnach erklären sich die Parallelen zwischen den Kathedralen von Lausanne und Genf einerseits und der gotischen Architektur im Burgund andererseits nicht durch direkte Abhangigkeit, sondern durch die gleichzeitige Obernahme anglonormannischer Formen. Ferner beschranken sich die Obereinstimmungen in der Architektur kleiner und mittelgrosser Bauten mit wenigen Ausnahmen auf die Realisierung desselben Bautypus mit Rechteckchor in verschiedenen Varianten, während die Ausführung im Detail lokalen Gewohnheiten folgt. Erwas engere Verbindungen zeigen sich lediglich zwischen den Kathedralen von Lausanne und Besançon sowie zwischen der Kollegiatskirche von Neuenburg und verschiedenen Bauten in der Freigrafschaft und im Burgund. Der westschweizerischen Gotik wesentlich näher stehen allerdings einige Kirchen im nördlichen Savoyen (Abondance, Evian-les-Bains etc.).The relations between gothic architecture in French-speaking Switzerland and Burgundy, by Stephan Gasser
A recent study by the present author of gothic architecture in French-speaking Switzerland has shown that the artistic relations often pointed out with Burgundian architecture and that or Franche-Comté should be rethought. The parallels drawn between Burgundian gothic architecture and the cathedrals of Lausanne and Geneva cannot be explained by direct dependency, but by simultaneous adaptation of Anglo-Norman forms. Furthermore, the affinities between the smaller or medium sized buildings are nearly always limited to the adaptation of the same type of flat chevet with a few variations, whereas the detailed execution is a product of local customs. Closer relations are visible only between the cathedrals of Lausanne and Besançon and between the collegiate church of Neuchâtel and a few buildings in Franche-Comté and Burgundy. Conversely, there is a close relation between the Gothic architecture in French-speaking Switzerland and a few churches in northern Savoy (Abondance, Evian-les Bains, etc.).Une recherche sur l'architecture gothique en Suisse romande récemment effectuée par l'auteur du présent article, a montré que l'étroitesse des relations artistiques avec la Bourgogne et la Franche-Comté, souvent évoquée, doit être remise en cause. Dès lors, les parallèles entre les cathédrales de Lausanne et de Genève d'une part, et l'architecture gothique en Bourgogne de l'autre, ne résulteraient pas d'une dépendance directe, mais d'une assimilation parallèle de certaines formes anglo-normandes. Les similitudes entre des édifices de petite ou moyenne dimension se limitent d'ailleurs le plus souvent à 1'adoption, avec quelques variantes, d'un même type de plan à chevet plat, tandis que, dans le détail, l'exécution demeure soumise aux habitudes locales. Il existe néanmoins des relations plus précises entre les cathédrales de Lausanne et de Besançon, ou entre la collégiale de Neuchâtel et quelques édifices comtois et bourguignons. Le gothique de la Suisse romande entretient en revanche des liens étroits avec certains édifices du nord de la Savoie, comme en témoignent les exemples d'Abondance, Evian-les-Bains etc.Gasser Stephan. Les relations entre l'architecture gothique eu Suisse romande et en Bourgogne. In: Bulletin Monumental, tome 164, n°2, année 2006. pp. 147-153
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