5,452 research outputs found
Bioluminescence Resonance Energy Transfer Reveals the Adrenocorticotropin (ACTH)-Induced Conformational Change of the Activated ACTH Receptor Complex in Living Cells
No full textBioluminescence resonance energy transfer analysis is used to study the interaction between melanocortin 2 receptor (MC2R) accessory protein and the MC2R and provides evidence for protein kinase A-dependent conformational changes in the receptor complex following receptor activation.
The melanocortin 2 receptor (MC2R) accessory protein (MRAP) is a small single-transmembrane domain protein that plays a pivotal role in the function of the MC2R. The pituitary hormone, ACTH, acts via this receptor complex to stimulate adrenal steroidogenesis. Using both coimmunoprecipitation and bioluminescence resonance energy transfer (BRET), we show that the MC2R is constitutively homodimerized in cells. Furthermore, consistent with previous data, we also show that MRAP exists as an antiparallel homodimer. ACTH enhanced the BRET signal between MC2R homodimers as well as MC2R-MRAP heterodimers. However, ACTH did not enhance the physical interaction between these dimers as determined by coimmunoprecipitation. Real-time BRET analysis of the MRAP-MC2R interaction revealed two distinct phases of the ACTH-dependent BRET increase, an initial complex series of changes occurring over the first 2 min and a later persistent increase in BRET signal. The slower ACTH-dependent phase was inhibited by the protein kinase A inhibitor KT5720, suggesting that signal transduction was a prerequisite for this later conformational change. The MRAP-MC2R BRET approach provides a unique tool with which to analyze the activation of this receptor
Dr. Carlsen as 'MC'
No full textDr. Charles J. Carlsen, the third president of JCCC poses as 'MC' in studi
Interactively using Semantic Web knowledge: Creating scalable abstractions with FacetOntology
No full textThe amount of knowledge accessible on the Semantic Web is growing, and there is a need for a scalable solution to facilitate exploring that data. Currently approaches to exploring Semantic Web data either focus on exploring resources individually, following links during exploration, and making little use of collated data, or take the approach of collating and aligning multiple sources into one store for one purpose, and hand-crafting a specific browsing interface onto it. We present an approach that provides a scalable browsing interface, which can browse knowledge from the Semantic Web at will. Our approach creates abstractions of knowledge, collated into facets, which are described using FacetOntology. FacetOntology facilitates describing facets from RDF data, suitable for use in creating datasets for faceted browsing
Growth Morphologies and Mechanisms of Non-Equilibrium Solidified MC Carbide
No full textGrowth morphologies and mechanisms of the carbide of group IVB and VB elements (MC carbide), a typical faceted crystal, were studied with an estimated cooling rate from 102 to 105 K/s. Results showed that although the growth morphologies of the MC carbide vary remarkably with solidification cooling rate, the solid/liquid interface is always atomically smooth, and the growth mechanisms are always lateral growth. The growth mechanism transition from lateral to continuous growth mode, which was predicted by the classic crystal growth theory, was not observed for the TiC type MC carbide within the estimated cooling rate range of 102?105 K/s
Ruth Klüger: la creatività per non “smarrirsi strada facendo”
No full textR. Klüger, a jewish viennese, a US citizen and a germanist, is the Author of two autobiographies “weiter leben”and “unterwegs versore”. Written in a style which alternates between essay and poetry, account and recollection, they are the occasion for the Author to establish a dialogue with the women having as a focus the exchange of experiences and reflections over the status of the feminine emancipation
Eutectic MC Carbide Growth Morphologies of a Laser Clad TiC/FeAl Composite Coating
No full textIn this paper, eutectic MC carbide growth morphology and its evolution with laser scanning speed were studied comprehensively of a laser clad MC carbide reinforced FeAl intermetallic matrix composite coating. As the laser scanning speed increased, the growth morphology of eutectic MC carbide was found to be needle-aligned annulation, butterfly-like and well-developed dendrite
Disclose
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“your darkness also/rich and beyond fear”: Community Performance, Somatic Poetics and the Vessels of Self and Other - Petra Kuppers. \ud
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"So what will you do on the plinth?”: A Personal Experience of Disclosure during Antony Gormley’s "One & Other" Project - Jill Francesca Dowse. \ud
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Food Confessions: Disclosing the Self through the Performance of Food - Jenny Lawson \ud
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Participation Cartography: The Presentation of Self in Spatio-Temporal Terms - Luis Carlos Sotelo-Castro \ud
Disclosure in Biographically-Based Fiction: The Challenges of Writing Narratives Based on True Life Stories - Donna Lee Brien. \ud
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Closure through Mock-Disclosure in Bret Easton Ellis’s Lunar Park - Jennifer Anne Phillips. \ud
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Disclosing the Ethnographic Self - Christine Lohmeier \ud
Celebrity Twitter: Strategies of Intrusion and Disclosure in the Age of Technoculture - Nick Muntean, Anne Helen Petersen.\ud
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“Just Emotional People”? Emo Culture and the Anxieties of Disclosure - Michelle Phillipov
Performance analysis of the MC-CDMA system in frequency selective slow Rayleigh fading channels, DS-CDMA or MC-CDMA?
No full textThis report describes the Bit Error Probability (BEP) performance of Multi-Carrier Code Division Multiple Access (MC-CDMA) system using the RAKE-receiver in frequency selective slow Rayleigh fading channels. The analysis has been carried out by a theoretical approach using the characteristic function method for quadratic form of Gaussian random variables and by computer simulation. The BEP performance of Direct Sequence Code Division Multiple Access (DS-CDMA) system under the same conditions is analysed and compared with the BEP performance of the MC-CDMA system.Electrical Engineering, Mathematics and Computer ScienceTelecommunicatie- en Verkeersbegeleidingssysteme
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