1,721,059 research outputs found
X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (<it>Ovis aries</it>) and European red deer (<it>Cervus elaphus</it>)
No full textAbstract Background Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous. Results A novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified. Conclusion PCR amplification using the amelogenin gene primers is useful in sex identification of samples from sheep and red deer and can be applied to DNA analysis of micro samples with small amounts of DNA such as hair roots as well as bones or embryo biopsies.</p
P5005 Hitchhiking effects influence allele frequencies and exclusion probabilities of microsatellites used for parentage control in Holstein Friesian cattle
No full textGlutamicibacter creatinolyticus strain LGCM 259 chromosome, complete genome
Get PDFNCBI Reference Sequence: NZ_CP034412.1
The complete genome sequencing of G. creatinolyticus LGCM 259 was deposited with the
National Center for Biotechnology Information (NCBI) under accession number CP034412; BioProject:
PRJNA507728, BioSample: SAMN10502625, Assembly: GCF_006094275.1 and the same became NCBI
RefSeq sequence NZ_CP034412.1. https://www.ncbi.nlm.nih.gov/nuccore/1679400616.
LOCUS NZ_CP034412 3309128 bp DNA circular CON 08JUN2019
DEFINITION Glutamicibacter creatinolyticus strain LGCM 259 chromosome,
complete genome.
ACCESSION NZ_CP034412
VERSION NZ_CP034412.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN10502625
Assembly: GCF_006094275.1
KEYWORDS RefSeq.
SOURCE Glutamicibacter creatinolyticus
ORGANISM Glutamicibacter creatinolyticus
Bacteria; Actinobacteria; Micrococcales; Micrococcaceae;
Glutamicibacter.
REFERENCE 1 (bases 1 to 3309128)
AUTHORS Santos,R.G., Silva,A.L., Seyffert,N., Castro,T.L.P., Attili,A.R.,
Rifici,C., Mazzullo,G., Brenig,B., Venanzi,F. and Azevedo,V.
TITLE Complete Genome Sequence of Glutamicibacter creatinolyticus strain
LGCM259,isolated from an abscess of a 12yearold
mare in Italy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3309128)
AUTHORS Santos,R.G., Silva,A.L., Seyffert,N., Castro,T.L.P., Attili,A.R.,
Rifici,C., Mazzullo,G., Brenig,B., Venanzi,F. and Azevedo,V.
TITLE Direct Submission
JOURNAL Submitted (07DEC2018)
General Biology, UFMG, Av Antonio Carlos
6627, Pampulha, Belo Horizonte, Minhas Gerais 31270901,
Brazil
COMMENT REFSEQ INFORMATION: The reference sequence was derived from
CP034412.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
Bacteria and source DNA available from GcLGCM259.
##GenomeAssemblyDataSTART##
Assembly Date :: OCT2018
Assembly Method :: SPAdes v. 3.9.1
Assembly Name :: GcLGCM259
Genome Representation :: Full
Expected Final Version :: Yes
Genome Coverage :: 286.0x
Sequencing Technology :: Illumina HiSeq
##GenomeAssemblyDataEND##
##GenomeAnnotationDataSTART##
Annotation Provider :: NCBI RefSeq
Annotation Date :: 06/07/2019 10:17:37
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Bestplaced
reference protein
set; GeneMarkS2+
Annotation Software revision :: 4.8
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 3,035
CDSs (total) :: 2,962
Genes (coding) :: 2,882
CDSs (with protein) :: 2,882
Genes (RNA) :: 73
rRNAs :: 4, 4, 4 (5S, 16S, 23S)
complete rRNAs :: 4, 4, 4 (5S, 16S, 23S)
tRNAs :: 58
ncRNAs :: 3
Pseudo Genes (total) :: 80
CDSs (without protein) :: 80
Pseudo Genes (ambiguous residues) :: 0 of 80
Pseudo Genes (frameshifted) :: 31 of 80
Pseudo Genes (incomplete) :: 51 of 80
Pseudo Genes (internal stop) :: 6 of 80
GenBank
Due to the large size of this record, sequence and annotated features are not shown. Use the "Customize view" panel to change the display##GenomeAnnotationDataEND##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..3309128
/organism="Glutamicibacter creatinolyticus"
/mol_type="genomic DNA"
/strain="LGCM 259"
/isolation_source="abcess of a mare"
/host="horse"
/db_xref="taxon:162496"
/country="Italy"
/collection_date="2015"
CONTIG join(CP034412.1:1..3309128)
/
Genetic polymorphism of the 17th exon at porcine RYR1 locus: a new variant in a local Portuguese pig breed demonstrated by SSCP analysis
No full textUntil 1991, malignant hyperthermia represented a major and important economic loss in modern pork production. It is a recessive autosomal disorder with variable expression that was. not known to occur in the local pig breeds from Portugal, Alentejano and Bisaro, prior to outcrossing with commercial breeds. In swine, this disorder is due to a mutation in exon 17 of the ryanodine receptor 1 (RYR1) gene, detectable through the analysis of a restriction fragment length polymorphism. We used this technique to distinguish between resistant (NN), carrier (Nn) and susceptible (nn) genotypes, with the aim of excluding carriers from the breeding programme. We further developed a rapid screening technique on the basis of SSCP (single-strand conformation polymorphism). Fifteen heterozygous (Nn) individuals were found in our sample of 48 individuals from Bisaro breed using both techniques. The absence of homozygous susceptible pigs su ts that the introduction of this allele into Bisaro breed has been recent. A genetically controlled breeding programme on the basis of our findings should help to secure the future of Bisaro pigs Of which few individuals are known to exist. To date, only one mutation was found in the 5 ' end of exon 17 of the porcine RYR1 gene. Here we present the frequencies of this mutation and describe a new mutation detected in the Alentejano breed. PCR-SSCP analysis followed by automatic sequencing allowed the detection of this rare transition (C --> T) at nucleotide 1851 of exon 17
Forensic DNA-typing of dog hair: DNA-extraction and PCR amplification
No full textThe forensic application of DNA-typing for the identification of dog hair provides objective evidence in the characterisation of traces found at crime scenes. During the past few years forensic dog identity testing has been improved considerably using multiplex PCR systems. However, DNA-typing from samples of one up to 10 dog hairs is often problematic in forensic science. A single dog hair contains very small quantities of DNA or the hair sample consists of hairs with roots of bad quality or even of broken hairshafts without roots. Here we describe an experimental study about dog hairs by means of a Ca2+ improved DNA-extraction method, quantification and amplification. (C) 2004 Elsevier Ireland Ltd. All rights reserved
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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