323,014 research outputs found
Extension to Data for: The use of FLIM for characterising chromosomes and their structure in response to low-dose X-ray irradiation
No full textmore files and extension to - https://doi.org/10.5286/edata/959 .In this repository are the raw and analysed data presented in the paper titled above. The experimental conditions are highlighted in the following published articles.
1. Botchway SW, Parker AW, Bisby RH, Crisostomo AG. Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation. Microscopy Research and Technique [Internet]. 2008 [cited 2025 Sep 9];71(4):267–73. Available from: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.20548
2. Ahmed A, Schoberer J, Cooke E, Botchway SW. Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells. Methods in molecular biology [Internet]. 2020 Dec 10 [cited 2025 Sep 9];287–301. Available from: https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_16
3. Stubbs CD, Botchway SW, Slater SJ, Parker AW. The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells. BMC Cell Biology [Internet]. 2005 Apr 26 [cited 2025 Sep 9];6(1). Available from: https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-6-22
4. Estandarte AK, Botchway S, Lynch C, Yusuf M, Robinson I. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes. Scientific Reports [Internet]. 2016 Aug 16 [cited 2025 Sep 9];6(1). Available from: https://www.nature.com/articles/srep31417
5. Berger SL, Phengchat R, Botchway SW, Yusuf M. Optimizing chromosome yield: a comparative analysis of harvesting, preparation and waste recovery methods. BioTechniques [Internet]. 2025 Jun 3 [cited 2025 Sep 9];77(5-6):245–56. Available from: https://www.tandfonline.com/doi/full/10.1080/07366205.2025.2536438?src=exp-la
Note: this deposit continues: https://doi.org/10.5286/edata/959N
Reflections on the United Kingdom’s first public health film festival
No full textFilm has become an integral part of everyday life in the UK, shaping our beliefs, culture and attitudes. Here, Stella Botchway from the Nuffield Department of Population Health, the University of Oxford and Uy Hoang from Kings College London, Public Health Film society and Nuffield Department of Population Health, University of Oxford discuss how film can be of interest to the public health community as well as the place for public health in film with reflections on the UK’s first public health film festival
Reflections on the United Kingdom’s first public health film festival
No full textFilm has become an integral part of everyday life in the UK, shaping our beliefs, culture and attitudes. Here, Stella Botchway from the Nuffield Department of Population Health, the University of Oxford and Uy Hoang from Kings College London, Public Health Film society and Nuffield Department of Population Health, University of Oxford discuss how film can be of interest to the public health community as well as the place for public health in film with reflections on the UK’s first public health film festival
Letter from A. A. Kidess to E. N. Teye-Botchway (October 16, 1970)
No full textThis is a copy of a two-page letter written by Attallah A. Kidess, Director of Springfield College's International Center, to E. N. Teye-Botchway, of the Department of Physical Education at the Specialist Training College in Winneba, Ghana. The letter is dated October 16, 1970. The letter talks about two International Students from Ghana who were attending Springfield College at the time, Dominic S. K. Agyei and Emmanuel B. Osei-Antwi.For more information on Attallah A. Kidess, see: https://springfield.as.atlas-sys.com/agents/people/579
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Directly imaging the localisation and photosensitization properties of the pan-mTOR inhibitor, AZD2014, in living cancer cells
No full textThe range of cellular functions the mechanistic target of rapamycin (mTOR) protein performs makes it an attractive drug target for cancer therapy. However, the cellular localisation and mode of action of second generation inhibitors of mTOR is poorly understood despite the level of attention there is in targeting the mTOR protein. We have therefore studied the properties of the pan-mTOR inhibitor AZD2014, an ideal candidate to study because it is naturally fluorescent, characterising its photochemical properties in solution phase (DMSO, PBS and BSA) and within living cells, where it localises within both the nucleus and the cytoplasm but with different excited state lifetimes of 4.8 (+/− 0.5) and 3.9 (+/− 0.4) ns respectively. We measure the uptake of the inhibitor AZD2014 (7 μM) in monolayer HEK293 cells occurring with a half-life of 1 min but observe complex behaviour for 3D spheroids with the core of the spheroid showing a slower uptake and a slow biphasic behaviour at longer times. From a cellular perspective using fluorescence lifetime imaging microscopy AZD2014 was found to interact directly with GFP-tagged mTORC1 proteins including the downstream target, S6K1. We observe light sensitive behaviour of the cells containing AZD2014 which leads to cell death, in both monolayer and spheroids cells, demonstrating the potential of AZD2014 to act as a possible photodynamic drug under both single photon and multiphoton excitation and discuss its use as a photosensitizer. We also briefly characterise another pan-mTOR inhibitor, INK128
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Author's address:
No full textCan archives of audiovisual TV interviews be used to make authors more visible to students, and thereby reduce the learning gap between native and non-native language speakers in college classes? We examined students in a college course who learned about one scholar's ideas through watching an audiovisual TV interview (i.e., visible author format) and about another scholar's ideas through reading a formal text description (i.e., invisible author format). For the invisible author, native language speakers scored significantly higher than the non-native language speakers on a corresponding exam question (i.e., a cognitive measure), generated more words on the exam question (i.e., a motivational measure), and mentioned the author's name more often in answering the exam question (i.e., an affective measure). For the visible author, the groups did not differ on any of these measures. These findings provide evidence for the idea that making the author visible through audiovisual TV interviews can eliminate the learning gap between native and non-native language speakers. 3 Universities around the world serve students who are non-native speakers of th
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