1,721,091 research outputs found
Supporting dataset for multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Full text linkMulti-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Jacopo Antonello, Aurélien Barbotin, Ee Zhuan Chong, Jens Rittscher, and Martin Booth
https://doi.org/10.1364/OE.393363
This folder contains the raw data acquired throughout the experiments. The data is grouped by figure number in the paper.
TIFF files are in OME-TIFF (https://docs.openmicroscopy.org/ome-model/latest/) format and can be opened with FiJi (https://imagej.net/Fiji/Downloads). The legend for the file name is --. So that xy-3dsted-aoon.tif corresponds to an xy scan along the lateral plane, in 3D STED mode, with adaptive optics (AO) correction applied.
AO log files contain the raw data acquired during the aberration correction experiments. These files are in HDF5 (https://www.hdfgroup.org/solutions/hdf5/) format and can be opened with a variety of applications (https://en.wikipedia.org/wiki/Hierarchical_Data_Format) that support HDF5 version 1.10 or later. The legend for the file name is ao---.h5. So that ao4-05-10-zsted.h5 corresponds to the fourth part of the aberration correction, where Zernike modes from 5 to 10 inclusive are corrected in ZSTED mode. The acquired images are stored at the address /stacks/stacks. The first image corresponds to the initial state of the correction. Next, the images necessary for the PN algorithm are collected. In the last image, the correction computed by the PN algorithm is applied. For each image, the corresponding column vector of Zernike coefficients applied by the DM is found at the address /dmlib/control/ZernikeControl/x
Continuous wave excitation two-photon fluorescence microscopy exemplified with the 647-nm ArKr laser line.
No full textWe report on efficient two-photon fluorescence imaging in beam scanning microscopy by exciting UV dyes at the 647-nm line of a continuous wave ArKr mixed gas laser. For a numerical aperture of 1.4 (oil), we used an illumination power of up to 210 mW at the sample. High-resolution images were obtained for DAPI-labelled cell nuclei within 4-60 s. Our method is a simple two-photon alternative to UV confocal imaging with the potential of becoming a very useful feature of laser scanning microscopy
Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope
Full text linkFig 5, 6 and
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Fluorescence Transients Acquired via Multiplane Imaging with AOD and Remote Focusing
No full textFluorescence transients from marked neurons at 120 um and 250 um shown in Fig. 8
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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