55 research outputs found
All-electron relativistic spin-orbit multireference computation to elucidate the ground state of CeH
No full textThe all-electron relativistic spin-orbit multiconfiguration/multireference computations with the Sapporo basis sets were carried out to elucidate the characters of the low-lying quasi-degenerate electronic states for the CeH diatomic molecule. The present computations predict the ground state of CeH to be a pure quartet state of 4f(1)5d(1)(5d(sigma)-H-1s)(2)6s(1) configuration (omega = 3.5). The first excited state (omega = 2.5) shows a doublet dominant of 4f(1)(5d(sigma)-H-1s)(2)6s(2) configuration at a short bond length while it changes to a quartet dominant at a long bond length. The Ce-H stretching fundamental frequency was calculated to be 1345 cm(-1) in the ground state, which is in good agreement with the experimental value, 1271 cm(-1), measured by a matrix-isolation technique
The cost of changing physical activity behaviour: Evidence from a "physical activity pathway" in the primary care setting
No full textCopyright @ 2011 Boehler et al.BACKGROUND: The ‘Physical Activity Care Pathway’ (a Pilot for the ‘Let’s Get Moving’ policy) is a systematic approach to integrating physical activity promotion into the primary care setting. It combines several methods reported to support behavioural change, including brief interventions, motivational interviewing, goal setting, providing written resources, and follow-up support. This paper compares costs falling on the UK National Health Service (NHS) of implementing the care pathway using two different recruitment strategies and provides initial insights into the cost of changing physical activity behaviour. METHODS: A combination of a time driven variant of activity based costing, audit data through EMIS and a survey of practice managers provided patient-level cost data for 411 screened individuals. Self reported physical activity data of 70 people completing the care pathway at three month was compared with baseline using a regression based ‘difference in differences’ approach. Deterministic and probabilistic sensitivity analyses in combination with hypothesis testing were used to judge how robust findings are to key assumptions and to assess the uncertainty around estimates of the cost of changing physical activity behaviour. RESULTS: It cost £53 (SD 7.8) per patient completing the PACP in opportunistic centres and £191 (SD 39) at disease register sites. The completer rate was higher in disease register centres (27.3% vs. 16.2%) and the difference in differences in time spent on physical activity was 81.32 (SE 17.16) minutes/week in patients completing the PACP; so that the incremental cost of converting one sedentary adult to an ‘active state’ of 150 minutes of moderate intensity physical activity per week amounts to £ 886.50 in disease register practices, compared to opportunistic screening. CONCLUSIONS: Disease register screening is more costly than opportunistic patient recruitment. However, additional costs come with a higher completion rate and better outcomes in terms of behavioural change in patients completing the care pathway. Further research is needed to rigorously evaluate intervention efficiency and to assess the link between behavioural change and changes in quality adjusted life years (QALYs).This article is available through the Brunel Open Access Publishing Fund
Author response: Analysis of the NK2 homeobox gene ceh-24 reveals sublateral motor neuron control of left-right turning during sleep
No full textSleep is a behavior that is found in all animals that have a nervous system and that have been studied carefully. In Caenorhabditis elegans larvae, sleep is associated with a turning behavior, called flipping, in which animals rotate 180{degree sign} about their longitudinal axis. However, the molecular and neural substrates of this enigmatic behavior are not known. Here, we identified the conserved NK-2 homeobox gene ceh-24 to be crucially required for flipping. ceh-24 is required for the formation of processes and for cholinergic function of sublateral motor neurons, which separately innervate the four body muscle quadrants. Knockdown of cholinergic function in a subset of these sublateral neurons, the SIAs, abolishes flipping. The SIAs depolarize during flipping and their optogenetic activation induces flipping in a fraction of events. Thus, we identified the sublateral SIA neurons to control the three-dimensional movements of flipping. These neurons may also control other types of motion
Manual therapies for cervicogenic headache: a systematic review
No full textThis paper systematically reviewed randomized clinical trials (RCT) assessing the efficacy of manual therapies for cervicogenic headache (CEH). A total of seven RCTs were identified, i.e. one study applied physiotherapy ± temporomadibular mobilization techniques and six studies applied cervical spinal manipulative therapy (SMT). The RCTs suggest that physiotherapy and SMT might be an effective treatment in the management of CEH, but the results are difficult to evaluate, since only one study included a control group that did not receive treatment. Furthermore, the RCTs mostly included participant with infrequent CEH. Future challenges regarding CEH are substantial both from a diagnostic and management point of view.
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited
Treatment of Cervicogenic Headache Concurrent With Cervical Stenosis by Anterior Cervical Decompression and Fusion
No full textStudy Design: Retrospective study. Objective: To report the efficacy of anterior cervical decompression and fusion surgery as treatment method for cervicogenic headache (CeH). Summary of Background Data: The exact diagnostic criteria and optimal treatment of CeH is still under investigation. Methods: A total of 34 consecutive patients (mean age 55.8 y) with CeH (in addition to cervical stenosis symptomatology) resistant to nonoperative treatment were treated by anterior cervical decompression and fusion from 1 up to 3 levels and were followed for at least 1 year. Clinical visual analog pain scale for headache, patient satisfaction index as well as radiographic examinations (flexion-extension radiographs and, when diagnosis of fusion status was uncertain, computed tomography) were documented for all patients at regular intervals. Statistical comparisons of outcome measures between different time points of examinations were performed. Results: All patients reported relief of their CeH with mean (range) visual analog pain scale scores as 8.1 (3-9), 2.4 (0-4), and 3.1 (0-5) preoperatively, at 2 months postoperatively, and at the final follow-up, respectively. There was a significant improvement (P < 0.001) of visual analog pain scale score between before surgery and at 2 months postoperatively or at the last follow-up. Thirty patients (88%) reported satisfied with their treatment, whereas 4 patients (12%) were not satisfied with surgery. No major surgical complication was seen and only 1 patient had symptomless pseudoarthrosis. Conclusions: CeH when associated with cervical spinal stenosis of the subaxial spine can improve when stenosis is treated with anterior cervical discectomy and fusion.SCI(E)ARTICLE8E1093-E10973
Growing Up with <i>Central European History</i>
No full textCentral European History (CEH) was the first scholarly journal I really got to know, and for more than thirty years, it has been important to me in all kinds of ways. I first encountered CEH as a Master's student at the University of Alberta, where my primary supervisor was the extraordinary Annelise Thimme, author of highly original works on Hans Delbrück, Gustav Stresemann, and the Deutschnationale Volkspartei. The discipline of history was new to me, and although I had taken some interesting undergraduate classes on early modern and modern history at the Universities of Saskatchewan and Munich, I had no idea about historiography, professional networks, or academic publishing. I probably did not even understand what the term Central Europe meant.</jats:p
Bentuk Penyajian Kesenian Didong Grup Musara Bintang di Desa Linung Bulen II Kecamatan Bintang Kabupaten Aceh Tengah
No full textThis research is entitled "Forms of Presentation of the Didong Arts of the Musara Bintang Group in Linung Bulen II Village, Bintang District, Central Aceh Regency". The author examines the form of presentation of Didong art by the Musara Bintang Group in Linung Bulen II Village, Bintang District, Central Aceh Regency. The research method used is a descriptive qualitative approach. Data obtained in the field used observation, interview and documentation techniques.The theory used in this research is the presentation theory from Djelantik's theory which states that form is the basic element of manifestation. In the form of presentation, it means that form is a fundamental element of a performance. These elements include artists, musicians, seating formation, movement, songs presented, costumes, performance venue, and audience. Based on the results of research in presentation, Didong art is played by 15 people consisting of: 1 Ceh Apit (Ceh Companion), 1 Main Ceh, and 2 Penepok Pumu and 2 Penepok pillow splits, each of which acts as a pillow act, split pillow , and 9 players who act as penepok/penunung (followers). The Didong art performance consists of 9 stages, for the costumes the players wear black unity t-shirts with the words "Musara Bintang" written on the chest and wear a headband called Jembolang
Alternative splicing controls pan-neuronal homeobox gene expression
No full textThe pan-neuronally expressed and phylogenetically conserved CUT homeobox gene ceh-44/CUX orchestrates pan-neuronal gene expression throughout the nervous system of Caenorhabditis elegans. As in many other species, including humans, ceh-44/CUX is encoded by a complex locus that also codes for a Golgi-localized protein, called CASP (Cux1 alternatively spliced product) in humans and CONE-1 ("CASP of nematodes") in C. elegans How gene expression from this complex locus is controlled-and, in C. elegans, directed to all cells of the nervous system-has not been investigated. We show here that pan-neuronal expression of CEH-44/CUX is controlled by a pan-neuronal RNA splicing factor, UNC-75, the C. elegans homolog of vertebrate CELF proteins. During embryogenesis, the cone-1&ceh-44 locus exclusively produces the Golgi-localized CONE-1/CASP protein in all tissues, but upon the onset of postmitotic terminal differentiation of neurons, UNC-75/CELF induces the production of the alternative CEH-44/CUX CUT homeobox gene-encoding transcript exclusively in the nervous system. Hence, UNC-75/CELF-mediated alternative splicing not only directs pan-neuronal gene expression but also excludes a phylogenetically deeply conserved golgin from the nervous system, paralleling surprising spatial specificities of another golgin that we describe here as well. Our findings provide novel insights into how all cells in a nervous system acquire pan-neuronal identity features and reveal unanticipated cellular specificities in Golgi apparatus composition.We thank Chi Chen for generating transgenic strains; John Calarco for sharing unpublished data; Barth Grant for shar ing the aman-2 reporter (RT2745 strain); Alberto Stolfi for discussion; HaoSheng Sun, Nuria Flames, and members of the Hobert laboratory and Leyva-Díaz laboratory for com menting on the manuscript; WormBase (Sternberg et al. 2024) and Caenorhabditis Genetics Center (funded by the National Institutes of Health Office of Research Infrastruc ture Programs, P40 OD010440) for providing resources and reagents. This work was funded by the Howard Hughes Medical Institute (O.H.) and by a GENT program grant for the Contratación de Investigadores Doctores de Excelencia from Generalitat Valenciana (CIDEXG/2022/30) (E.L.-D.). Author contributions: E.L.-D. and O.H. conceived the project and designed the experiments. E.L.-D. and M.C. performed all the experiments, imaging, and quantifica tions, except for those specified. K.P. conducted the exper iments, imaging, and quantifications shown in Figure 4B and Supplemental Figure S5A. J.I.J.-L. and J.V. performed the quantifications shown in Figures 1D and 2E. The man uscript was prepared by E.L.-D., M.C., and O.H.Peer reviewe
Homeoprotein Hbx4 represses adhesion molecule governing cytokinesis and development
No full textHomeobox genes encode proteins with a highly conserved DNA-binding motif and provoke morphological diversification of body segments by differentially controlling the expression of downstream targets. Here, we have identified _hbx4_, one of many homeobox genes in _Dictyostelium discoideum_ and investigated its role during growth and development. In suspension, Hbx4-overexpressing cells, Hbx4^OE^, showed defects in cytokinesis and growth rate. During development, Hbx4^OE^ and _hbx4_-disrupting cells, _hbx4¯_ made differences in shape of mound and slug, cell-type proportioning from wild type KAx3 cells. These phenotypes were similar to those of mutant defective in _cadA_ encoding Ca^2+^-dependent cell adhesion molecule so that we investigated the relationship between _hbx4_ and _cadA_. Overexpression of Hbx4 inhibited the expression of _cadA_ and cAMP also failed to stimulate _cadA_ in Hbx4^OE^. Furthermore, gel mobility shift assay showed the promoter of _cadA_ contained Hbx4-binding site, indicating Hbx4 negatively regulates the expression of _cadA_. Proteome analysis revealed that overexpression of Hbx4 repressed the _rdiA_ and _abpB_ encoding rho guanine nucleotide dissociation inhibitor1, RhoGDI1 and actin bundling protein 34, ABP34, respectively. And the overexpression of _cadA_ in Hbx4^OE^ cells rescued the defects and increased mRNA level of _rdiA_, _abpB_ and one of Rho GTPase, _rac1b_. These results suggested that Hbx4 can modulate cytokinesis, cell sorting and cell-type proportioning by repressing _cadA_ that regulates GTPase-dependent signaling pathway
Interactions between LHX3-And ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
No full text© 2017 The Author(s). LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional 'LIM code' that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Islet1 (ISL1), is essential for the development of motor neurons. Using yeast two-hybrid analysis we showed that the Caenorhabditis elegans orthologs of LHX3 and ISL1, CEH-14 and LIM-7 can physically interact. Structural characterisation of a complex comprising the LIM domains from CEH-14 and a LIM-interaction domain from LIM-7 showed that these nematode proteins assemble to form a structure that closely resembles that of their vertebrate counterparts. However, mutagenic analysis across the interface indicates some differences in the mechanisms of binding. We also demonstrate, using fluorescent reporter constructs, that the two C. elegans proteins are co-expressed in a small subset of neurons. These data show that the propensity for LHX3 and Islet proteins to interact is conserved from C. elegans to mammals, raising the possibility that orthologous cell specific LIM-HD-containing transcription factor complexes play similar roles in the development of neuronal cells across diverse species
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