1,720,962 research outputs found
Developmental stimuli and stress factors affect expression of ClGLP1, an emerging allergen-related gene in Citrus limon.
No full textbe involved in many developmental and stress related processes. A novel GLP cDNA was isolated from
Citrus limon and structural features and genomic organization were investigated by in silico and Southern
blots analysis. In lemon, the ClGLP1 encodes a 24.38 kDa which possesses a conserved motif of plant GLPs
proteins. A phylogetic analysis mapped ClGLP1 as belonging to the GER3 subfamily into the GLP1 group of
large GLP family. ClGLP1 was differentially expressed in the various organs and was highest in mature
fruit. Moreover, expression in the fruit was tissue- and stage-related as well as dependent on agricultural
practice (organic vs conventional). ClGLP1 transcripts increased during the transition from the green (180
days after blooming) to the yellow (240 days after blooming) mature fruit and were strongly enhanced in
yellow mature fruit from organic compared with conventional culture. A sudden and systemic increase in
ClGLP1 expression level was observed in leaves injured by wounding, together with an increase of
endogenous H2O2 amount. Notably, an enhancement of H202 was observed in fruit peel during transition
from green to yellow fruit stage. All together our data showed that ClGLP1 expression can be modulated
in relation to both developmental stimuli and culture practices; evidence is also provided that through
an oxidase activity this gene could play a role in fruit maturation as well as in stress responses
Cytological studies on shoot apical meristem during transition from juvenile to adult phase in Olea europaea L.
No full textShort- and long-term effects of dehydroascorbate in Lupinus albus and Allium cepa roots
No full textAdministration of 1 mM dehydroascorbate (DHA) results in a rapid and large increase in cellular ascorbate (AA) content in both Lupinus albus L. and Allium cepa L. root tips. Uptake of DHA from the medium occurs at a high rate within 10-12 h of incubation, and is slowed down thereafter. In the first few h, DHA reduction to AA is apparently correlated to GSH depletion and slightly higher DHA reductase activity. DHA incubation also seems to induce new GSH synthesis. Longer DHA incubation (24 h) affects root growth by inhibiting cell proliferation. At this stage, an apparently generalised oxidation of SH-containing proteins is observed in DHA-treated roots. Treatment with 1 mM L-galactono-gamma-lactone, the last precursor of AA biosynthesis, results in an increase in AA content similar to that obtained with DHA, but stimulates growth and affects the redox state of SH-containing proteins in the opposite way. A possible multi-step mechanism of DHA reduction/removal is suggested and the hypothesis that DHA inhibits cell cycle progression by affecting the redox state of SH-containing proteins is discussed
Distribuzione delle citochinine e pattern di metilazione del DNA marcano la transizione dalla fase giovanile alla vegetativa adulta in Prunus persica (L.) Batsch.
No full textExpression of Arabidopsis WEE1 in tobacco induces unexpected morphological and developmental changes
No full textWEE1 regulates the cell cycle by inactivating cyclin dependent protein kinases (CDKs) via
phosphorylation. In yeast and animal cells, CDC25 phosphatase dephosphorylates the CDK releasing
cells into mitosis, but in plants, its role is less clear. Expression of fission yeast CDC25 (Spcdc25) in
tobacco results in small cell size, premature flowering and increased shoot morphogenetic capacity in
culture. When Arath;WEE1 is over-expressed in Arabidopsis, root apical meristem cell size increases,
and morphogenetic capacity of cultured hypocotyls is reduced. However expression of Arath;WEE1 in
tobacco plants resulted in precocious flowering and increased shoot morphogenesis of stem explants,
and in BY2 cultures cell size was reduced. This phenotype is similar to expression of Spcdc25 and is
consistent with a dominant negative effect on WEE1 action. Consistent with this putative mechanism,
WEE1 protein levels fell and CDKB levels rose prematurely, coinciding with early mitosis. The phenotype
is not due to sense-mediated silencing of WEE1, as overall levels of WEE1 transcript were not reduced
in BY2 lines expressing Arath;WEE1. However the pattern of native WEE1 transcript accumulation
through the cell cycle was altered by Arath;WEE1 expression, suggesting feedback inhibition of native
WEE1 transcription
Transizione dalla fase giovanile alla fase vegetativa adulta in Prunus persica: aspetti morfometrici ed immunocitochimici.
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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