1,720,964 research outputs found
Replication data for: A transcriptomic analysis in mice following a single dose of ibogaine identifies new potential therapeutic targets
No full textData from transcriptomic analysis conducted on frontal cortex tissue of mice acutely exposed to ibogaine (60 mg/kg). Frontal cortex samples were obtained after four hours of oral administration. Samples were prepared for RNA sequencing. Fist of all, RNA was extracted using the Purelink RNA mini kit and quantified by a Qubit 2.0 Fluorometer. After that, the quality of the RNA was assessed using the Agilent TapeStation team and the Agilent RNA ScreeTape Assay. Sequencing libraries were created from 0.75 μg of RNA samples using the Illumina Stranded mRNA Prep and quantified by microfluidic electrophoresis using Agilent's TapeStation equipment and the Agilent DNA High Sensitivity ScreenTape kit. The length and concentration were determined in each sample. Finally, pools with a concentration of 750 pM were created. These pool sequencing libraries were done using NextSeq200 equipment from Illumina. The obtained data was mapped against a reference genome by the alignment program HISAT2 2.2.1, and annotation and quantification of the reads was done by the StringTie 2.1.4. Three database were generated: Counts_All.csv, Counts_Females.csv and Counts_Males.csv, the first being the main file with all the data obtained, while the other two present modifications. The control and IBO groups of men or women were eliminated according to the interest of the analysis. Gene database was screened for outliers which were then eliminated and analyzed using R 4.3.0 and its package DESeq 1.40.1
Influence of prenatal chlorpyrifos exposure, APOE genotype and sex on neurodevelopmental disorders: behavioral and biochemical abnormalities in mice
Full text linkL'ús massiu del pesticida clorpirifós (CPF) pot desencadenar efectes perjudicials sobre la salut, en particular, en la població en desenvolupament. En els darrers anys, s'ha associat l'increment en el diagnòstic dels trastorns de l'espectre autista (TEA) amb l'exposició a tòxics. D'altra banda, existeixen polimorfismes genètics com el de l'apolipoproteina E (APOE) que influeixen en la resposta a factors ambientals. En aquesta tesis s'ha avaluat com l'exposició prenatal a CPF i el gentotip APOE contribueixen en les alteracions conductuals i bioquímiques observades en els trastorns del neurodesenvolupament. Les femelles C57BL/6J i homozigòtiques pels al·lels humans e3 i e4 es van exposar a través de la dieta a 0 o 1 mg/kg/dia de CPF des de el dia gestacional (DG) 12 al 18. A més, un grup de femelles C57BL/6J es va exposar a 300 mg/kg/dia d'àcid valporic (AVP) els DG 12 i 13, per mitjà d'una injecció subcutànea. Un altre grup de C57BL/6J va ser exposar per vía oral a 0 o 1 mg/kg/dia de CPF del dia postnatal 10 al 15. En els individus exposats prenatalment es va avaluar la cura de les cries i la qualitat del niu en les mares. Les habilitats comunicatives i el desenvolupament físic i motor durant el període de lactància, i les conductes d'ansietat durant l'adolescència, així com l'expressió d'elements involucrats en el sistema GABAergic i glutamatergic. Les conductes socials es van avaluar en els ratolins adolescents exposats pre o postnatalment a CPF. En l'avaluació de les habilitats comunicatives dels ratolins C57BL/6J, vam observar que els efectes provocats per l'exposició a CPF eren oposats als observats en l'exposició a AVP, però els dos tractaments van produir un retard en el desenvolupament físic, alteracions en les conductes socials i canvis en l'expressió d'elements GABAergics i glutamatèrgics de manera dependent del sexe. A més. al llarg de l'estudi es van observar diferencies del genotip APOE, a excepció de les conductes socials on ambdós genotips van mostrar una fectació del tractament de manera dependent del sexe. En general aquesta tesis qüestiona l'associació entre el CPF i el TEA, i suggereix que les diferents isoformes d'apoE (apoE3 i apoE4) no són un factor de risc pel desenvolupament del trastorn.El uso masivo del pesticida clorpirifos (CPF) puede desencadenar efectos perjudiciales sobre la salud, en particular, en la población en desarrollo. En los últimos años, se ha asociado el incremento en el diagnóstico de los trastornos del espectro autista (TEA) con la exposición a tóxicos. Por otra parte, existen polimorfismos genéticos como el de la apolipoproteína E (APOE) que influyen en la respuesta a factores ambientales. En esta tesis se ha evaluado cómo la exposición prenatal a CPF y el genotipo APOE contribuyen en las alteraciones conductuales y bioquímicas observadas en los trastornos del neurodesarrollo. Las hembras C57BL/6J y homocigóticas por los alelos humanos e3 y e4 se expusieron a través de la dieta a 0 o 1 mg/kg/día de CPF des de el día gestacional (DG) 12 al 18. Además, un grupo de hembras C57BL/6J se expusieron a 300 mg/kg/día de ácido valproico (AVP) los DG 12 y 13, por medio de una inyección subcutánea. Otro grupo de C57BL/6J se expuso por via oral a 0 o 1 mg/kg/día de CPF del día postnatal 10 a 15. En los individuos expuestos prenatalmente se evaluó el cuidado de las crías y la calidad del nido en las madres. Las habilidades comunicativas y el desarrollo físico y motor durante el período de lactancia, y las conductas de ansiedad durante la asolescencia, así como la expresión de elementos involucrados en el sistema GABAergico y glutamatérgico. Las conductas sociales se evaluaron en los ratones adolescentes expuestos pre o postnatalmente a CPF. En la evaluación de las habilidades comunicativas de los ratones C57BL/6J observamos que los efectos provocados por la exposición a CPF eran opuestos a los observados en la exposición a AVP, pero ambos tratamientos produjeron un retraso en el desarrollo físico, alteraciones en las conductas sociales y cambios en la expressión de elementos GABAergicos y glutamatérgico de forma dependiente del sexo. Además, a lo largo del estudio, se observaron diferencias del genotipo APOE, a excepción de las conductas sociales donde ambos genotipos mostraron una afectación del tratamiento de forma dependiente del sexo. En general, esta tesis cuestiona la asociación entre el CPF y el TEA, y sugiere que las distintas isoformas de apoE (apoE3 y apoE4) no son un factor de riesgo para el desarrollo del trastorno.The massive use of the pesticide chlorpyrifos (CPF) can trigger detrimental health effects, specially, in the developing population. In recent years, the increase in the diagnosis of autism spectrum disorder (ASD) has been associated with exposure to toxics. On the other hand, there are genetic polymorphism such as that of apolipoprotein E (APOE) that influence the response to environmental factors. In this thesis we have evalauted how prenatal exposure to CPF and the APOE genotype contribute to the behavioral and biochemical alterations observed in neurodevelopmental disorders. Females C57BL/6J and homozygous for the human e3 and e4 alleles were exposed trough the diet to 0 or 1 mg/kg/day of CPF from gestational day (GD) 12 to 18. In addition, a group of C57BL/6J females were exposed to 300 mg/kg/day of valproic acid (VPA) on GD 12 and 13, by a subcutaneous injection. Another group of C57BL/6J mice was orally exposed to 0 or 1 mg/kg/day of CPF from postnatal day 10 to 15. Dams of mice prenatally exposed were evaluated in maternal care and nest quality. Communication skills and physical and motor development were evalauted during lactation period, and anxiety behavior during adolescence, as well as the expression of elements involved in the GABAergic and glutamatergic system. Social behavior was assessed in adolescent mice exposed pre or postnatally to CPF. The effects induced in communication skills by prenatal CPF exposure were opposite to those observed with VPA in C57BL/6J mice, but both treatments delayed physical development, altered social behavior and changed the expression of some GABAergic and glutamatergic elements in a sex-dependent manner. Moreover, throughout the study, we observed differences between APOE genotype, except for social behavior, where both genotypes showed an affectation of the treatment depending on sex. In general, this thesis questions the association between CPF and ASD, and suggests that the different apoE isoforms (apoE3 and apoE4) are not a risk factor for the development of the disorder
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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