31 research outputs found
Author Correction: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
No full textMechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
No full textNeurons enhance their computational power by combining linear and nonlinear transformations in extended dendritic trees. Rich, spatially distributed processing is rarely associated with individual synapses, but the cone photoreceptor synapse may be an exception. Graded voltages temporally modulate vesicle fusion at a cone’s ~20 ribbon active zones. Transmitter then flows into a common, glia-free volume where bipolar cell dendrites are organized by type in successive tiers. Using super-resolution microscopy and tracking vesicle fusion and postsynaptic responses at the quantal level in the thirteen-lined ground squirrel, Ictidomys tridecemlineatus, we show that certain bipolar cell types respond to individual fusion events in the vesicle stream while other types respond to degrees of locally coincident events, creating a gradient across tiers that are increasingly nonlinear. Nonlinearities emerge from a combination of factors specific to each bipolar cell type including diffusion distance, contact number, receptor affinity, and proximity to glutamate transporters. Complex computations related to feature detection begin within the first visual synapse
The Saccharomyces cerevisiae phosphatidylinositol/phosphatidylcholine transfer protein functions as a negative feedback regulator of the CDP-choline pathway
No full textThe Saccharomyces cerevisiae phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein (SEC14p) is required for Golgi function in vivo. Mutations that specifically result in the inactivation of the CDP-choline pathway for PC biosynthesis bypass the requirement for SEC14p. The in vivo consequences of SEC14p dysfunction have been shown to result in the CDP-choline pathway driven increase in Golgi PC content. Overproduction of the SEC14p is found to manifest a decrease in the cellular PC content due to an inhibition of the CDP-choline pathway. This phenotype is reversed in cells that also overproduce the yeast cholinephosphatecytidylyltransferase (CCTase). The SEC14p is shown to act as a negative regulator of the CDP-choline pathway through the ligand-modulated SEC14p dependent inhibition of CCTase. PC bound SEC14p is identified as likely the active agent in the inhibition of CCTase. SEC14p is further shown to be conserved throughout the yeasts. A gene for the Schizosaccharomyces pombe SEC14p was cloned and its nucleotide sequence determined. The S. pombe SEC14p is highly homologous to the S. cerevisiae SEC14p and functionally replaces the S. cerevisiae SEC14p in vivo.Made available in DSpace on 2011-05-07T12:00:46Z (GMT). No. of bitstreams: 2
license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5)
9522178.pdf: 3582327 bytes, checksum: b171d46cf618377553c8564ed3a07fd7 (MD5)
Previous issue date: 1995Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding ([email protected]) on 2011-05-07T14:35:29Z
Item is restricted indefinitely.Restriction data tranferred 2014-07-01T11:14:00-05:00
Original Data
Group with Access UIUC Users [automated]
Release Date: none
Reason: ETDs are only available to UIUC Users without author permissionETDs are only available to UIUC Users without author permissionU of I Onl
Abstract B103: Transparent tumor tomography (T3): Spatial 3D mapping of immune responses in a whole tumor after immunotherapy
No full text
The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
No full textThe secretory pathway of the yeast Saccharomyces cerevisiae is strictly analogous to that of mammalian cells. Proteins destined for secretion are transported in a vectorial fashion from the endoplasmic reticulum to the Golgi apparatus to the cell surface. Superimposed on the normal flow of secretory traffic in yeast is a level of spatial organization. Golgi-derived secretory vesicles are directed to a defined region of the mother cell surface known as the bud. During the budding portion of the cell cycle, secretion and cell surface growth are coincident which results in the selective growth of the bud. The actin cytoskeleton has been implicated as the mediator of the polarized mode of yeast cell growth. The filamentous actin cytoskeleton consists of two structures, asymmetrically-arranged cortical patches and cables which are aligned along the mother cell-bud axis. Structural analyses indicated that the patches could participate in localized membrane growth while the cables are correctly positioned to be involved in directed vesicular transport. Given the proposed relationship between secretion and actin, it seems reasonable that there would be communication between the secretory pathway and the actin cytoskeleton in yeast. Presented in this thesis is evidence that the S. cerevisiae SAC1 gene product could represent one aspect of the mechanism for coupling secretory pathway function and actin assembly in yeast. Mutations in SAC1 were isolated as extragenic suppressors of both Golgi and actin defects. Analysis of the SAC1 gene product revealed that the SAC1p was a 71kD integral membrane protein that exhibited a small cytoplasmic domain. The SAC1p localized to yeast ER and Golgi membranes, but showed no obvious association with the filamentous actin cytoskeleton. Native immunoprecipitation experiments suggested that the SAC1p was an actin binding protein in yeast. Finally, a model is proposed which reconciles how the SAC1p could be involved in the activities of both the secretory pathway and actin cytoskeleton, thereby rendering the SAC1p capable of participating in the spatial restriction imposed on secretory traffic in yeast.Made available in DSpace on 2011-05-07T13:52:59Z (GMT). No. of bitstreams: 2
license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5)
9215797.pdf: 4240595 bytes, checksum: eb5c46700aef146d4bebb2c633425468 (MD5)
Previous issue date: 1992Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding ([email protected]) on 2011-05-07T15:00:19Z
Item is restricted indefinitely.Restriction data tranferred 2014-07-01T11:28:31-05:00
Original Data
Group with Access UIUC Users [automated]
Release Date: none
Reason: ETDs are only available to UIUC Users without author permissionETDs are only available to UIUC Users without author permissionU of I Onl
Abstract Connections
No full textThis thesis links disparate sources to build a new way of looking at abstract painting. It draws on anthropology, cognitive science, philosophy, and art history to open up a different discourse about abstraction. Alfred Gell’s anthropological theory of art is used as a model for investigating the various social exchanges that take place in the entire spectrum of interactions that abstract painting involves. These social transactions include both traditional linguistic ones, as well as non-linguistic exchanges. Cognitive science is looked at to investigate the experience of sensation at a more granular level than that of language. Philosophy, particularly of Gilles Deleuze, is discussed in order to tie sensation with the opening of a fissure to connect to new ways of thinking and debating. Art History is
investigated to situate my own practice with other painters, both historical and contemporary. This openness of sources in my writing is reflective of the openness of the types of resources that I use in my painting practice, where I mix ornamentation, topology,
and gesture. In both the written thesis and my art practice the goal is to regard abstraction as a means to facilitate alternative knowledge production. In this sense, abstraction is investigated as a search for discursiveness through a material practice
The donation of King Mindaugas to the Lithuanian bishop christian: considerations on a possible controversy
No full textThe Christianisation of Lithuania is usually dated to the late 14th and early 15th centuries, but the first attempt to turn Lithuania into a Christian kingdom was made by Mindaugas. In 1251 he was baptised by the Master of Livonia Andreas de Velven (also known as von Stirland), and in 1253 was crowned King of Lithuania with the support of Pope Innocent IV. Christian (Kristijonas), the Livonian priest who taught the ruler the virtues of Christianity, a member of the Teutonic Order, became the first Bishop of Lithuania at Mindaugas’ request. It is known that Mindaugas bequeathed several lands to Christian in 1254. The article examines the background to this act of providing for the Diocese of Lithuania. The author argues that the donation of the possessions to Christian is the most important source that can help identify Mindaugas’ own domain. He hypothesises that Mindaugas’ domain may have been located south of the lands donated to Bishop Christian, in an area known in historiography as the defence line of the lower River Nemunas. In his analysis of this hypothesis, the author presents arguments that invite us to reconsider the established assertion in historiography that the domains of the senior dukes could have been located in present-day east Lithuania.Humanitarinių mokslų fakultetas / Faculty of HumanitiesVytauto Didžiojo universitetas / Vytautas Magnus Universit
Real-life Application of TEX and Adobe Acrobat for Electronic Publishing: a Handbook of Algebra and a Journal Archive
No full text. A classical way of using T E X in printed typesetting was enhanced for use of the same T E X source to publish electronically. A handbook of algebra and a 4-year journal archive (280 articles) were electronically published using the same T E X source files to produce both the PDF in a form for reading on-screen and a version for printing a hard copy. A package written in plain T E X provided for the mark-up of the logical structure, cross-references, bibliographical references, author names, keywords and symbols. The hypertext contents, index pages and a complete navigation system are also made in PDF and were pre-programmed at the T E X level. Being completely a PDF product the same publications are thus usable on any computer system for which a PDF viewer exists. 1. Introduction "The entry of T E X into the world of hypertext" (as described by Y. Haralambous and S. Rahtz in 1995, see [1]) was probably facilitated by several factors, among which we see the rise of L A T E X, the ..
ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms
No full text© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Casler, J. C., Zajac, A. L., Valbuena, F. M., Sparvoli, D., Jeyifous, O., Turkewitz, A. P., Horne-Badovinac, S., Green, W. N., & Glick, B. S. ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Molecular Biology of the Cell, (2020): mbcE20090591, doi:10.1091/mbc.E20-09-0591.Membrane traffic can be studied by imaging a cargo protein as it transits the secretory pathway. The best tools for this purpose initially block export of the secretory cargo from the endoplasmic reticulum (ER), and then release the block to generate a cargo wave. However, previously developed regulatable secretory cargoes are often tricky to use or specific for a single model organism. To overcome these hurdles for budding yeast, we recently optimized an artificial fluorescent secretory protein that exits the ER with the aid of the Erv29 cargo receptor, which is homologous to mammalian Surf4. The fluorescentsecretory protein forms aggregates in the ER lumen and can be rapidly disaggregated by addition of a ligand to generate a nearly synchronized cargo wave. Here we term this regulatable secretory proteinESCargo (Erv29/Surf4-dependent Secretory Cargo) and demonstrate its utility not only in yeast cells, but also in cultured mammalian cells, Drosophila cells, and the ciliate Tetrahymena thermophila. Kinetic studies indicate that rapid export from the ER requires recognition by Erv29/Surf4. By choosing an appropriate ER signal sequence and expression vector, this simple technology can likely be used withmany model organisms.This work was supported by NIH grant R01 GM104010 to BSG, by NIH grant R01 GM105783 to APT, by NIH grant R01 GM136961 and American Cancer Society grant RSG-14-176 to SHB, and by NIH grant R01 DA044760 to WNG. JCC was supported by NIH training grant T32 GM007183. AZ was supported by American Heart Association fellowship 16POST2726018 and American Cancer Society fellowship 132123-PF-18-025-01-CSM. Thanks for assistance with fluorescence microscopy to Vytas Bindokas and Christine Labno at the Integrated Microscopy Core Facility, which is supported by the NIH-funded Cancer Center Support Grant P30 CA014599. The pUASt-ManII-eGFP plasmid was a gift from Bing Ye, and the Ubi-Gal4 plasmid was a gift from Rick Fehon.2020-12-2
