19 research outputs found
Fish distribution dynamics in the Aghanashini estuary of Uttara Kannada, west coast of India
No full textFish diversity (77 species) in the Aghanashini River estuary of the Indian west coast is linked to variable salinity conditions and zones I, II and III for high, medium and low salinity respectively. Zone I, the junction between Arabian Sea and the estuary, had all species in yearly succession due to freshwater conditions in monsoon to high salinity in pre-monsoon. The medium (zone II) and low (zone III) salinity mid and upstream portions had maximum of 67 and 39 fish species respectively. Maintenance of natural salinity regimes in estuary, among other ecological factors, is critical for its fish diversity
From non-Brahmin priests of the goddess to ascetics of god Mahima Alekha
No full textThis article deals with Mahima Dharma a contemporary vernacular ascetic religion of Odisha/Eastern India displaying a rich diversity in its regional configurations. In this paper the author proposes to look at the main protagonists of the religion, the ascetics (babas), as non-Brahmin priests, who have incorporated shakti, the power of local goddesses into their disciplined bodies and in doing so have transformed the feminine element of the Hindu belief into the belief of the indescribable and abstract god Alekha. Mahima Dharma is seen in this contribution as a sort of micro structure on the one hand of popular asceticism in rural India and on the other hand as a recent religious reform movement integrating local non-Brahmin priesthood and the local belief in goddesses into the mainstream of the male Hindu pantheon. This article draws on the author's PhD fieldwork research (1999-2002), published in 2002 as a monograph (Guzy 2002)
Medical image fusion of different modalities using dual tree complex wavelet transform with PCA
No full textProvisions, Applicability and Recent Amendments To The Maharashtra Stamp Act, 1958
No full textThis paper deals with the Maharashtra Stamp Act, 1958 and the recent amendments related to the same. Stamp Duty is an important part of Land Law and the relevant provisions, application and relevant judgments of the same are discussed in the paper. Stamp duty is charges by the government levied on documents or instruments which have certain transactional nature which forms a part of the government exchequer. It is required to pay the adequate stamp duty as regards to the Maharashtra Stamp Act, which mentions the rate of stamp duty applicable on specific instruments such as transfer, agreement, conveyance, etc. Recent cases of stamp duty evasion have been rising in the State of Maharashtra which has led to certain amendments in the Maharashtra Stamp Act, 1958 which has also been discussed herein. The author has taken up the case law of Trustees of H.C. Dhanda Trust v. State of Madhya Pradesh and Ors of 2020, to analyze one such case of stamp duty evasion and has commented upon the judgment delivered therein. This paper duly encompasses all aspects of stamp duty in consonance with the Maharashtra Stamp Act, 1958 and discusses the measures which could bring about more clarity upon the current laws of stamp duty and consequence of evasion, in accordance with the stamp duty law in the state of Maharashtra
Tracking Cancer Genetic Evolution using OncoTrack
No full textabstract: It is difficult for existing methods to quantify, and track the constant evolution of cancers due to high heterogeneity of mutations. However, structural variations associated with nucleotide number changes show repeatable patterns in localized regions of the genome. Here we introduce SPKMG, which generalizes nucleotide number based properties of genes, in statistical terms, at the genome-wide scale. It is measured from the normalized amount of aligned NGS reads in exonic regions of a gene. SPKMG values are calculated within OncoTrack. SPKMG values being continuous numeric variables provide a statistical metric to track DNA level changes. We show that SPKMG measures of cancer DNA show a normative pattern at the genome-wide scale. The analysis leads to the discovery of core cancer genes and also provides novel dynamic insights into the stage of cancer, including cancer development, progression, and metastasis. This technique will allow exome data to also be used for quantitative LOH/CNV analysis for tracking tumour progression and evolution with a higher efficiency.The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep2964
Influence of protein damage and proteasome gene expression in longevity of recalcitrant Madhuca latifolia Roxb. seeds
No full textEnhanced cellular damage during desiccation is considered to be one of the key factors limiting vigour and viability of seeds. The uncontrolled accumulation of reactive oxygen species and resultant damaging reactions such as the oxidation of lipids and DNA in desiccating seeds of Madhuca latifolia (Roxb.) J. F. Macbr. has already been well characterized. However, hydrolytic and (or) oxidative damage to proteins requires further study. This study investigated the desiccation-induced oxidative damage to proteins and proteolytic systems in recalcitrant M. latifolia seeds during ambient storage. Seeds experienced a significant drop in seed water content [ca. 1.32 to ca. 0.23 g·(g dry mass)−1] during storage resulting in complete loss of viability after 35 days of storage. A considerable decline in total protein content (3.0–3.6 fold) and activity (4.8–13.8 fold) in the gene expressions of proteasome subunits (α, β, and E2) were recorded in the embryonic axis of desiccating M. latifolia seeds. In contrast, increases in the level of protein carbonyls (2.46 fold), hydroperoxides (2.31 fold), malondialdehyde- and 4-hydroxy-2-nonenal-protein adducts (1.8 and 3.9 fold), and Amadori and Maillard reaction products, along with proteases (14.5–30.4 fold) were observed in desiccating M. latifolia seeds. This study revealed that increased oxidation/modification of proteins and proteasome dysfunction are involved in the deterioration of desiccating M. latifolia seeds.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Biological evaluation and in silico molecular docking study of a new series of thiazol-2-yl-hydrazone conglomerates
No full textCathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover
No full textCathepsin D (CTSD) is a lysosomal aspartic protease and its inherited deficiency causes a severe pediatric neurodegenerative disease called neuronal ceroid lipofuscinosis (NCL) type 10. The lysosomal dysfunction in the affected patients leads to accumulation of undigested lysosomal cargo especially in none-dividing cells, such as neurons, resulting in death shortly after birth. To explore which proteins are mainly affected by the lysosomal dysfunction due to CTSD deficiency, Lund human mesencephalic (LUHMES) cells, capable of inducible dopaminergic neuronal differentiation, were treated with Pepstatin A. This inhibitor of “acidic” aspartic proteases caused accumulation of acidic intracellular vesicles in differentiating LUHMES cells. Pulse-chase experiments involving stable isotope labelling with amino acids in cell culture (SILAC) with subsequent mass-spectrometric protein identification and quantification were performed. By this approach, we studied the degradation and synthesis rates of 695 and 680 proteins during early and late neuronal LUHMES differentiation, respectively. Interestingly, lysosomal bulk proteolysis was not altered upon Pepstatin A treatment. Instead, the protease inhibitor selectively changed the turnover of individual proteins. Especially proteins belonging to the mitochondrial energy supply system were differentially degraded during early and late neuronal differentiation indicating a high energy demand as well as stress level in LUHMES cells treated with Pepstatin A
Structural, crystallographic, Hirshfeld surface, thermal and antimicrobial evaluation of new sulfonyl hydrazones
No full textColloid Transport in Porous Media at Multiple Length Scales
No full textColloidal contaminants such as pathogenic microorganisms and engineered nanoparticles enter subsurface from various sources such as land application of wastewater, reuse of untreated sewage for irrigation and sanitary landfills. Understanding colloid transport in the subsurface is essential for assessing the safe distance of drinking water wells from the source of contamination, bioremediation of contaminated sites, and degree of treatment required for land application of wastewater. There is a large disparity in the length scales associated with colloid transport in soil. This includes micrometre, centimetre, tens of centimetres, metre and kilometre scales which are representative of a single soil capillary, representative elementary volume scale, 1D lab scale, 3D lab scale, and the field scale, respectively. Colloid deposition mechanisms are scale dependent and are governed by the heterogeneity at that scale. Hence, the observed transport processes and the estimated parameters at a smaller scale may not simulate the observations at a larger scale. It is imperative to link the processes and the associated parameters across scales to better predict transport behavior at larger scales. This chapter discusses the mechanisms of colloid retention in porous media at various scales, the effect of heterogeneity on colloid transport at each scale, and upscaling of transport processes. © 2022, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd
