312 research outputs found
SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing
No full textPre-mRNA splicing is catalyzed by the spliceosome, a multi-megadalton ribonucleoprotein machine. Previous work from our laboratory revealed the splicing factor SRSF1 as a regulator of the SUMO pathway, leading us to explore a connection between this pathway and the splicing machinery. We show here that addition of a recombinant SUMO-protease decreases the efficiency of pre-mRNA splicing in vitro. By mass spectrometry analysis of anti-SUMO immunoprecipitated proteins obtained from purified splicing complexes formed along the splicing reaction, we identified spliceosome-associated SUMO substrates. After corroborating SUMOylation of Prp3 in cultured cells, we defined Lys 289 and Lys 559 as bona fide SUMO attachment sites within this spliceosomal protein. We further demonstrated that a Prp3 SUMOylation-deficient mutant while still capable of interacting with U4/U6 snRNP components, is unable to co-precipitate U2 and U5 snRNA and the spliceosomal proteins U2-SF3a120 and U5-Snu114. This SUMOylation-deficient mutant fails to restore the splicing of different pre-mRNAs to the levels achieved by the wild type protein, when transfected into Prp3-depleted cultured cells. This mutant also shows a diminished recruitment to active spliceosomes, compared to the wild type protein. These findings indicate that SUMO conjugation plays a role during the splicing process and suggest the involvement of Prp3 SUMOylation in U4/U6U5 tri-snRNP formation and/or recruitment.Fil: Pozzi, María Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Bragado, Laureano Fabian Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Will, Cindy L.. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Mammi, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Risso, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Urlaub, Henning. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Lührmann, Reinhard. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Srebrow, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentin
Correction to: Free Diced Dorsal Augmentation (FDDA) rhinoplasty in non-caucasian patients: tips and tricks (European Journal of Plastic Surgery, (2025), 48, 1, (7), 10.1007/s00238-024-02259-1)
No full textIn this article the author’s name ‘Gianluca Marcaccini’ was incorrectly written as ‘Gianlcua Marcaccini’. Authors ‘Mirco Pozzi’ and ‘Pietro Susini’ should have been denoted as equally contributing author[s]. The original article has been corrected
Knowledge-based document filing
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Athens/Shibboleth
This paper appears in:
IEEE Expert
Date of Publication: Oct. 1993
Author(s): Pozzi, S.
Cefriel, Milano, Italy
Celentano, A.
Volume: 8 , Issue: 5
Page(s): 34 - 45
Product Type: Journals & Magazines
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ABSTRACT
The Kabiria document management system, which represents the knowledge needed by document producers and users as a component of document semantics, is discussed. Kabiria supports the classification, filing, and retrieval of office documents through three levels of knowledge representation: a static level, bound to the structure and textual content of documents; a procedural level, related to the operational aspects of office work; and a domain level, related to the specific application domain and its governing regulations. While documents are represented at a deep level of detail, the office context of procedures, agents, events, and regulations is represented in terms of elements that are modeled according to their links to document
La Società Umanitaria e la diffusione del Metodo Montessori (1908-1923)
No full textThe Società Umanitaria ofMilan, between 1918 and 1923, played an essential role in spreading and developing the Montessori Method. Studying in the Historical Archive of Società Umanitaria the numerous documents there collected, the author reconstructed the crucial moments of the extremely significant collaboration between Maria Montessori and Augusto Osimo, General Secretary of the Società Umanitaria.This complex and in-depth investigation was guided by the analysis, in specific, of the training courses for Montessori teachers organised by Società Umanitaria, essentially unexamined before this study, that allowed the researcher to have a deep insight into the action of Società Umanitaria aimed to promote and implement the Montessori Method in Italy and all around the world
Heidegger ai margini. Antropologia e trasgressione dello spazio urbano: un caso studio
No full textThe Programa Especial de Realojamento (PER) offers the opportunity to the municipalities situated in Lisbon and Porto metropolitan areas of succeeding in eliminating clandestine neighborhoods and providing the reallocation of the residents in social houses. The decree contextualizes the housing problem as «a still open plague in our social fabric».The current process implementation, more than twenty-year far from the original formulation, has produced complex adaptation, resistance and fighting dynamics. Through the analyses of an ethnographic case study, the author problematizes the implementation of this governance program, focusing on the consequent systematic demolition of informal settlements in the Lisbon periphery under the aegis of the urban renewal doctrine
The PEP survey: evidence for intense star-forming activity in the majority of radio-selected AGN at z ≳ 1
No full textIn order to investigate the far-infrared (FIR) properties of radio-active active galactic nuclei (AGN), we have considered three different fields where both radio and FIR observations are the deepest to date: GOODS-South, GOODS-North and the Lockman Hole. Out of a total of 92 radio-selected AGN, ~64 per cent are found to have a counterpart in Herschel maps. The percentage is maximum in the GOODS-North (72 per cent) and minimum (~50 per cent) in the Lockman Hole, where FIR observations are shallower. Our study shows that in all cases FIR emission is associated with star-forming activity within the host galaxy. Such an activity can even be extremely intense, with star-forming rates as high as ~103-104 M⊙ yr-1. AGN activity does not inhibit star formation in the host galaxy, just as on-site star formation does not seem to affect AGN properties, at least those detected at radio wavelengths and for z ≳ 1. Given the very high rate of FIR detections, we stress that this refers to the majority of the sample: most radio-active AGN are associated with intense episodes of star formation. However, the two processes proceed independently within the same galaxy, at all redshifts but in the local universe, where powerful enough radio activity reaches the necessary strength to switch off the on-site star formation. Our data also show that for z ≳ 1 the hosts of radioselected star-forming galaxies and AGN are indistinguishable from each other in terms of both mass and IR luminosity distributions. The two populations only differentiate in the very local universe, whereby the few AGN which are still FIR-active are found in galaxies with much higher masses and luminosities
Sarah Bernhardt's 'Doctor God': Jean-Samuel Pozzi (1846-1918)
No full textSamuel Pozzi was a major figure in the early development of modern gynaecological surgery. His textbook, A Treatise on Gynaecology, published in French in 1890 and rapidly translated into five other languages, was the first internationally acclaimed text integrating modern principles of anaesthesia, antisepsis, diagnosis, surgical technique and postoperative care, and in later editions remained a standard reference up to the 1930s. He was the author of more than 400 papers on gynaecological and general abdominal surgery and his technical expertise drew surgeons from all over the world to his theatre in the Hospital Broca, in one of the poorer parts of Paris. He was equally successful in several professional fields apart from medicine. However, his name is now little known in the English-speaking world. This short biography aims to re-introduce Pozzi to readers of English
Novecento e postmodernità nella critica musicale di Fedele d'Amico. Riflessioni sul metodo storiografico di un corso universitario
No full textThe article examines a university course held in 1978 by the music critic Fedele d’Amico (Rome, 1912 - Rome, 1990) at the Faculty of Letters of the University of Rome La Sapienza. The course offers a reading of the twentieth century, through the analysis of the main movements and composers of the first half of the century, highlighting the historiographical method and the teacher’s critical positions. The historiographical method of Fedele d’Amico attempts to identify the dominant character of the twentieth century through a comparison with nineteenth-century music. This character resides, according to d’Amico, in the general tendency of 20th century composers to be «anti-nineteenth century», to mark the discontinuity with respect to the previous century. In the more radical experiences of modernity represented above all by expressionism, dodecaphony and the serial avant-garde of the Darmstadt mouvement, d’Amico underlines the destruction of the linguistic character of music which, according to him, is the foundation of understanding in musical listening. The author, emphasizing the reductionism of some of d’Amico’s statements, avoids a generic charge of conservatism by relating them to the crisis of modernism and to the spread, in the criticism of the arts during the seventies, of a new postmodern image of the twentieth century
Il simbolismo floreale in Antonia Pozzi e Krystyna Krahelska
No full textThough raised in different cultures and countries, the poetesses Antonia Pozzi and Krystyna Krahelska share a similar view of nature. In their works, flower symbolism plays a significant role, occasionally influenced by folklore and literature, but most often resulting from their own reinterpretations of established meanings. The author focuses on plants as metaphors of the self, as images of life and death, and as a language to describe erotic desire and unrequited love
Mario Luzi e l'arte: da Simone Martini ai contemporanei
No full textThis contribution deals with Luzi's career as a connoisseur and critic of figurative arts: starting from Luzi's precocious piece of criticism on Raffaello, the author investigates other works by the Italian writer dealing with art history, theayhre and poetry. The works of great artists like Simone Martini, Jacopo Carracci (alias Pontormo) and Giorgio De Chirico werw a source of inspiration for Luzi's Viaggio celeste e terrestre di Simone Martini, Felicità turbate and Avvento notturno
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