158,695 research outputs found
Ethylene signalling receptors and transcription factors over the grape berry development: gene expression profiling
No full textThe ethylene signalling pathway has never been fully described in grapes. Regarded as a non-climacteric fruit, grape berry seems to ripen independently to ethylene, however 1-methylcyclopropene (1-MCP), a specific inhibitor of ethylene receptors has been shown to alter berry ripening processes. Here, we report profiles of transcript abundance of various receptors and transcription factors, associated with ethylene signalling, throughout berry development. Transcript abundance of ortholog VvETR2 gene showed a transient peak at the inception of ripening in 'Cabernet Sauvignon' berries coinciding with an internal ethylene peak, prior to colour changes. The transcripts of other orthologs such as VvRTE1 and VvEIN4 steadily increased over the berry development, while VvERS1 ortholog transcripts exhibited a peak of accumulation only when the berries were fully coloured. Finally, mRNAs of two transcription factors, VvEIN3 and VvMADS4, showed strong accumulation during the late phase of berry ripening. We also observed inflections of mRNA accumulation after incubating berry clusters with ethylene and 1-MCP (inhibitor of ethylene action). The main effect was observed with VvEIN3 transcripts that showed a significant up-regulation after incubation with 1-MCP. Furthermore, other transcript levels (VvETR2 and VvCTR1) were also increased by exogenous ethylene, once the colour change was initiated (i.e. 10 to 11 weeks after bloom). Some studies have already indicated that non-climacteric fruits shared signalling pathways with climacteric fruits. However, most differences between these ripening classifications remain poorly described at the genetic/molecular level. This data set will contribute to a better understanding on potential involvements of ethylene signalling in a non-climacteric fruit such as grape berry
Coffee berry disease in Kenya
No full textData are presented on research in Kenya in 1964 - 1969 on anatomical, mycological, epidemiological, chemical control and cultural aspects of coffee berry disease, Colletotrichum coffeanum Noack, of Coffea arabica L. The pathogen causes flower and berry losses and was found in branches where it occupied clearly defined areas of the cortex just before or after formation of the first phellogen. Saprophytic Colletotrichum spp. inhabit bark areas with more periderms in the cortex. No relationship could be found in Kenya between Glomerella cingulata (Stonem.) Sp. & Schr., the perfect stage of most of the saprophytic Colletotrichum bark components, and C. coffeanum . The seasonal fluctuations in pathogenicity in the bark population of C. coffeanum could be assessed and compared with the total sporulating capacity of the bark population of all Colletotrichum spp. Formerly the level of this total sporulating capacity, or 'inoculum potential' as it was then called, was used as an indication when pre-rain copper sprays had to be applied and how effectively the fungicide had reduced the bark inoculum. Based on these data the recommendations for chemical control were changed from pre-rain fungicide applications, to a spraying regime well into the rainy period, the accent being on protection of the berries rather than on a reduction of the bark inoculum. The fungicide Ortho Difolatan proved to be more effective than copper based compounds. Cultural practices, like the application of high levels of fertilizers, manure and mulch and rigid pruning practices, had no effect on the level of C. coffeanum in branches. Copper containing fungicides pushed the Colletotrichum balance in favour of C. coffeanum . Berries from non-copper sprayed coffee fields were less susceptible to standard conidial suspensions of C. coffeanum than berries from copper sprayed trees. A similar effect of fungicides should be considered in South and Central American coffee growing countries, where the application of fungicides has increased tremendously since the occurrence of Hemileia vastatrix Berk. et Br. in Brazil
Vascular functioning and development of the kiwifruit berry (Actinidia deliciosa)
No full textThe aim of this study was to understand kiwifruit berry development and the role of cell turgor and the phloem unloading pathway in development. Important aspects of berry development include the size of the fruit and its composition. The fresh weight growth curve of the kiwifruit berry was shown to be double sigmoid in shape. Dry weight accumulated linearly for the initial 139 days after anthesis (DAA). At this time the soluble solids concentration began to increase. Berry firmness was measured using two methods, with the penetrometer and with a new non-destructive method, utilising skinfold callipers. Both methods exhibited similar results, indicating that the skin callipers may be useful in the future for non-destructive berry rheological measurements.
Cell turgor was measured indirectly from measurements of symplasmic and apoplasmic solute potentials, and the matric potential of the berry. Apoplasmic sap, required to measure the apoplasmic solute potential, was extracted using two different methods the pressure chamber and through centrifugation. Measurements of sap osmotic potential suggest that the sap extracted using the centrifuge was contaminated with symplasmic sap, resulting in a negative cell turgor estimate. However, the pressure chamber technique provided apoplasmic sap that produced a more accurate estimate of cell turgor. Direct estimates of cell turgor were only obtained from the midpoint of the growing season because of contamination with symplasmic sap, but the values obtained were comparable to literature values for developing grape and tomato berries.
The phloem unloading pathway in the fruit was investigated using a symplasmic tracer dye, carboxyfluorescein diacetate coupled with 14C labelling and autoradiography. The phloem unloading pathway was symplasmic until 91 DAA when the dye was restricted to the phloem cells only, indicating a change to an apoplasmic pathway. However, due to the lack of functional unloading seen in radiolabelled samples, a change in the phloem unloading pathway could not be confirmed
Acai Berry Extracts Can Mitigate the L-Glutamate-Induced Neurotoxicity Mediated by N-Methyl-D-Aspartate Receptors
No full textBackground/Objectives: Stroke is the second leading cause of death worldwide. There is an unmet need to manage stroke pathophysiology, including L-glutamate (L-Glu)-mediated neurotoxicity. The acai berry (Euterpe sp.) contains phytochemicals with potentially nutraceutical content. The aim of this study was to assess the ability of acai berry extracts to counter L-Glu neurotoxicity using human differentiated TE671 cells. Methods: The cytotoxicity of L-Glu and acai berry extracts was quantified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Mitochondrial function was examined by a quantitation of cellular ATP levels, the maintenance of the mitochondrial membrane potential (MMP), and the production of reactive oxygen species (ROS). Whole-cell patch-clamp recordings monitored the activation of N-methyl-D-aspartate receptors (NMDARs). Candidate phytochemicals from acai berry extracts were modeled in silico for NMDAR binding. Results: L-Glu significantly reduced cell viability, ATP levels, the MMP, and increased cellular ROS. Generally, acai berry extracts alone were not cytotoxic, although high concentrations were detrimental to ATP production, maintenance of the MMP, and elevated ROS levels. Whole-cell patch-clamp recordings revealed that the combined addition of 300 µM L-Glu and 10 µM glycine activated currents in differentiated TE671 cells, consistent with triggering NMDAR activity. Acai berry extracts ameliorated the L-Glu-induced cytotoxicity, mitochondrial dysfunction, elevated ROS levels, and limited the NMDAR-mediated excitotoxicity (p < 0.001–0.0001). Several virtual ligands from acai berry extracts exhibited high-affinity NMDAR binding (arginine, 2,5-dihydroxybenzoic acid, threonine, protocatechuic acid, and histidine) as possible candidate receptor antagonists. Conclusions: Acai berry phytochemicals could be exploited to reduce the L-Glu-induced neurotoxicity often observed in stroke and other neurodegenerative diseases
Berry, D A, VX4798
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/371571Surname: BERRY
Given Name(s) or Initials: D A
Military Service Number or Last Known Location: VX4798
Missing, Wounded and Prisoner of War Enquiry Card Index Number: 6668182066
Item: [2016.0049.03898] "Berry, D A, VX4798
Berry, M D, 3796356
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/371551Surname: BERRY
Given Name(s) or Initials: M D
Military Service Number or Last Known Location: 3796356
Missing, Wounded and Prisoner of War Enquiry Card Index Number: SEA-4672182046
Item: [2016.0049.03878] "Berry, M D, 3796356
Perseverance: Bestselling Author Is Cedarville’s Berry Chair of Entrepreneurship
No full textNearly 18 years ago, Kary Oberbrunner scribbled “10-23-05 Cedarville?” next to Psalm 75 in his Bible. Now, 12 books, two companies, and one doctoral degree later, this Wall Street Journal and USA Today bestselling author and entrepreneur is serving as the Berry Chair of Entrepreneurship
Sex Addiction: the Chicken-and-Egg Dilemma of Diagnosis
No full textIn May 2013, the American Psychiatric Association will release the next version of the Diagnostic and Statistical Manual of Mental Disorders (DSM-V). Interestingly, sex addiction, despite significant attention from mainstream media, will be omitted from the manual. This omission presents a challenge to clinicians who treat sex addiction, and researchers aiming to further our understanding of the issue. This commentary outlines some of the reasons sex addiction was not included in the DSM-V, including a ‘chicken-and-egg’ conundrum, which makes it difficult to generate research without a clear diagnosis, and difficult to establish a definitive diagnosis without a supportive body of research
Maj. Berry D Brazile
No full textBust image of Major Berry D. Brazile, son of Mrs. Delia W. Brazile of 2500 University Dr., has ended a 30-day leave after returning from Korea and is on duty at his new post, Lockbourne Air Base near Columbus, Ohio. Published in Fort Worth Star-Telegram evening edition July 1951.https://mavmatrix.uta.edu/specialcollections_startelegram1950s/13413/thumbnail.jp
Berry phase in neutrino oscillations
No full textWe study the Berry phase in neutrino oscillations for both Dirac and Majorana neutrinos. In order to have a Berry phase, the neutrino oscillations must occur in a varying medium, the neutrino-background interactions must depend on at least two independent densities, and also there must be CP violation. If the neutrino interactions with matter are mediated only by the standard model W and Z boson exchanges, these conditions imply that there must be at least three generations of neutrinos. The CP violating Majorana phases do not play a role in generating a Berry phase. We show that a natural way to satisfy the conditions for the generation of a Berry phase is to have sterile neutrinos with active-sterile neutrino mixing, in which case at least two active and one sterile neutrinos are required. If there are additional new CP violating flavor changing interactions, it is also possible to have a nonzero Berry phase with just two generations.Astronomy & AstrophysicsPhysics, Particles & FieldsSCI(E)0ARTICLE5null7
- …
