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Fascaplysinopsis , Bergquist 1980
No full textGenus <i>Fascaplysinopsis</i> Bergquist, 1980 <p> <b>Definition</b>: Superficially armoured Thorectinae, with a dense collagenous matrix supported by a skeleton of thick cored heavily laminated primary fibres, uncored laminated secondary and tertiary fibres, often with pithing.</p> <p> <b>Diagnosis.</b> Lobate upright digitate fistular and semi burrowing sponges, with a superficial armour and rounded conules. The skeleton is semi-regular but characterised by large diameter detritus cored fasciculating laminated primary fibres, uncored often pithed, laminated secondary fibres and tertiary fibres. The primary fibres can be 2 to 10 times the diameter of the secondary fibres. The tertiary fibres can also be 2 to 10 times smaller than the secondary fibres. The mesohyl is characteristically gelatinous due to the collagenous matrix, but the consistency is firm and compressible.</p> <p> <b>Remarks.</b> The genus <i>Fascaplysinopsis</i> was created by Bergquist (1980) to accommodate irregular branched thorectid sponges with detritus-cored fasciculated primary fibres and uncored secondary fibres. This was meant to differentiate species from <i>Aplysinopsis</i> that have large fasciculated primary fibres, lack a sand cortex, and have a cavernous internal skeleton. When doing this, Bergquist designated <i>Aplysinopsis reticulata</i> Hentschel, 1912 as the type species, which had previously been synonymised with <i>Fasciospongia cavernosa</i> (Schmidt, 1862) by de Laubenfels (1948: 123), the latter action rejected by Bergquist (1980). Unfortunately, in defining <i>Fascaplysinopsis</i>, Bergquist relied heavily on recently collected material from Queensland, Australia. Here, we examined the syntype of <i>A. reticulata</i> (SMF 904) and compared it to the specimens from Queensland described by Bergquist (1980) and used in the illustration of the type species of <i>Fascaplysinopsis</i> (Bergquist 1980: Fig. 16 A–B), which is here attributed to a new species from a new genus of Thorectinae, <i>Skolosachlys</i> <b>gen. nov</b>. In addition, the material from New Caledonia and northern Queensland later added by Bergquist (1995: 18) to <i>F. reticulata,</i> is also attributed here to a new species from another new genus of Thorectinae, <i>Rubrafasciculus</i> <b>gen. nov.</b> Thus, <i>Fascaplysinopsis</i> is restricted to a monotypic genus containing only its type species, <i>F. reticulata</i> (Hentschel, 1912), but having its generic definition amended based on re-examination of the syntype of <i>A. reticulata</i>, here designated as the lectotype. The addition of new species <i>F. palauensis</i> <b>sp. nov.</b>, <i>F. klobos</i> <b>sp. nov.</b> and <i>F. ronquinni</i> <b>sp. nov.</b> brings the total number of species in <i>Fascaplysinopsis</i> to four.</p>Published as part of <i>Ekins, Merrick, Erpenbeck, Dirk, Debitus, Cécile, Petek, Sylvain, Mai, Tepoerau, Wörheide, Gert & Hooper, John N. A., 2023, Revision of the genus Fascaplysinopsis, the type species Fascaplysinopsis reticulata (Hentschel, 1912) (Porifera, Dictyoceratida, Thorectidae) and descriptions of two new genera and seven new species, pp. 201-241 in Zootaxa 5346 (3)</i> on page 207, DOI: 10.11646/zootaxa.5346.3.1, <a href="http://zenodo.org/record/8390072">http://zenodo.org/record/8390072</a>
Lamellomorpha strongylata Bergquist 1968
No full textLamellomorpha strongylata Bergquist, 1968 Figs 1–2, 6; Tables 1–2 Lamellomorpha strongylata Bergquist, 1968 (in part): 31–32, pls 4a, 11e–f, fig. 10. Lamellomorpha strongylata — Cryer et al. 2000: 21, appendix 8a–b. — Hooper & Maldonado 2002: 165 –167, fig. 1. — Hickford 2007: 40. — Kelly et al. 2009: 42. — NABIS 2017: 1 –4. ‘ Lamellomorpha n. sp. K & W’ in Cryer et al. 2000: 42 (NIWA 51169 leg.). Type material Holotype NEW ZEALAND • Northeast of Three Kings Islands, NZOI Station B 93; 33.983° S, 172.350° E; depth 54–109 m; 22 Oct. 1958; NIWA 356 (NZOI H–33) leg.; beam trawl; UPSZTY 178600 (a piece of the holotype preserved in 70% ethanol, as well as a spicule preparation), NIWA. Other material examined NEW ZEALAND – Northeast of Three Kings Islands, NIWA Station Z 9678 (KAH9901 /27); 34.360° S, 172.712° E; depth 48 m; 26 Jan. 1999; NIWA 51169 and 51172 leg.; UPSZMC 178601 (fragment of NIWA 51172 leg. preserved in 70% ethanol), NIWA • Northeast of Three Kings Islands, NIWA Station Z 9686 (KAH9901 /43); 34.361° S, 172.686° E; depth 48 m; 27 Jan. 1999; NIWA 51267 leg.; UPSZMC 178603 (fragment preserved in 70% ethanol), NIWA • Northeast of Three Kings Islands, NIWA Station Z 9699 (KAH9901 /67); 34.360° S, 172.673° E; depth 41 m; 28 Jan. 1999; NIWA 51438 leg.; NIWA • Northeast of Three Kings Islands, NIWA Station Z 9710 (KAH9901 /85); 34.353° S, 172.765° E; depth 54 m; 28 Jan. 1999; NIWA 51582 leg.; dredge; NIWA • Three Kings Islands, 2.5 nm east of Great Island, NIWA Station Z15944; 34.170° S, 172.210° E; depth 200 m; 16 Apr. 1999; CRRF, NIWA 93474 leg.; dredge; NIWA • Spirits Bay, Northland, NIWA Station KAH0606/D3; 34.36° S, 172.847° E; 15 May 2005; NIWA 52375 leg.; dredge; NIWA. • Middlesex Bank, Three Kings Rise, NIWA Station TAN1105/43; 33.988° S, 171.751° E; depth 170–174 m; 28 Mar. 2011; NIWA 73243, 73253 leg.; beam trawl; NIWA • Western Continental Slope, Northland, NZOI Station J 954 (I808); 34.633° S, 172.225° E; depth 204– 192 m; 18 Jun. 1981; collected by rock dredge; specimen now lost, donated by Dame P. R. Bergquist to Dr P. Karuso, Macquarie University, Sydney. Description The holotype was described by Bergquist (1968) as a “massive, thick, sometimes folded and incurved lamellate sponge”, 130 mm high, 102 mm wide, and 18–22 mm thick, supported by a stout stalk 30 mm in diameter. The surface was described as smooth where the dermal membrane was intact, otherwise ragged due to projecting clumps of oxeas and strongyles. Oscules, 1–2.6 mm in diameter, were found on the convex surface of the lamella and lie flush with the surface (Bergquist 1968). Examination of the numerous preserved specimens in NIC reveal occasional membranous oscules, but it is difficult to tell whether they are restricted to one side of the sponge. However, in the holotype, pores were observed on the opposite side to the oscules, in cribriporal areas, separated by small ridges, or with no boundaries, making a continuous pore surface; each pore is 40–80 µm in diameter. The texture was described as, “firm but compressible, crisp, easily broken”. The colour in life was described as “bright green” and the colour in spirit, “blue green or yellowish green” (Bergquist 1968). The most recent collection was by the Coral Reef Research Foundation in 1999 (NIWA 93474 leg.; Fig. 2A), who described a “dark, royal blue (not navy blue), (palmate) fan sponge with pointed tips, 20 cm high and about 1 cm thick, that tears easily, and which has a fleshy surface”. Skeleton The description by Bergquist (1968) of the choanosome as “lax and confused with slight traces of radiate construction discernible”, is accurate, but in NIWA 93474 leg. the contort strongyles strongly radiate through the plane of the fan. Bergquist described a “subectosomal region”, in which there were tracts of megascleres, variable in thickness, that curved outward and intersect with the surface at an acute angle; in NIWA 93474 leg. these are predominantly oxeas (Fig. 6A). The ectosome is densely packed with microstrongyles and streptasters, which also occur throughout the sponge, but in much less abundance. Spicules MEGASCLERES (Table 1; Fig. 2D) Bergquist (1968) considered the megascleres of L. strongylata (Bergquist 1968: 31, 32 (table of spicule dimensions)) to be “strongyles, oxeas and strongyloxeas”, all of similar range in length and width, varying only in relative frequency in the two specimens (presumably the Three Kings holotype and the NZOI Station B 176 specimen from Campbell Plateau), with oxeas being dominant in the latter. Re-examination of the holotype megascleres, and those of more recent collections, indicate that there are probably two forms of megascleres: 1) straight to slightly curved oxeas that are common in the subectosomal tracts, ranging from about 1500–1750 µm long and up to 25 µm thick; and 2) massive sinuous or contort oxeas that are usually very thick and frequently modified with one or both ends rounded as in strongyloxeas, rarely as in true strongyles, ranging from about 1600–2375 µm long and up to 40 µm thick. However, it is difficult to distinguish the various megascleres in some specimens, and in some the spicules are much less contort. MICROSCLERES (Table 2; Fig. 2 C–F) Microstrongyles are “squat, evenly rounded spicules, slightly roughened and occasionally centrotylote” (Bergquist 1968: 31, 32 (table of spicule dimensions)) and range from about 21–34 µm long (Table 2). Bergquist described the streptaster microscleres of L. strongylata (Bergquist 1968: 31, 32 (table of spicule dimensions)) as “plesiasters, small spicules with 3–12 smooth, sharply pointed rays”. A reexamination of the holotype (Fig. 2 E–F) using scanning electron microscopy has revealed that the streptaster microscleres are metasters and occasionally amphiasters with relatively long microspined rays, all in one size category, following the definition of Sollas (1888), and as used in Cárdenas & Rapp (2012). We describe these spicules as metaster- to amphiaster-like streptasters with heavily spined, relatively long rays in one size category, ranging in length from about 7–15 µm long (Table 2). Distribution Northeast of New Zealand. Substrate, depth range and ecology Attached to rocky reefs and sediment and rubble-covered rocky platforms, depth 41– 200 m. DNA barcodes COI. NIWA 51172 leg. (minibarcode, MK033624) and NIWA 51267 leg. (MK033623): no bp differences. 28S (C1-C2). NIWA 51172 leg. (MK 033143) and NIWA 51267 leg. (MK 033142): no bp differences. We failed to get sequences from the holotype. Remarks Lamellomorpha strongylata was originally described in considerable detail by Bergquist (1968), and the holotype was redescribed without re-examination more recently by Hooper & Maldonado (2002). No further material was examined. Here, for the first time, we illustrate the sponge as it appears upon collection, showing the beautiful royal blue colouration (Fig. 2A), and illustrate the detail and ornamentation of the microscleres (Fig. 2 C–F) using scanning electron microscopy. There is little to add to the original description, consequently the description and skeletal details are provided in comparative prose. Lamellomorpha strongylata is restricted to the northernmost tip of New Zealand and beyond to the Three Kings Rise, and is easily recognised in the field by the palmate, tree-like shape and the deep blue to green colouration.Published as part of Kelly, Michelle, Cárdenas, Paco, Rush, Nicola, Sim-Smith, Carina, Macpherson, Diana, Page, Mike & Bell, Lori J., 2019, Molecular study supports the position of the New Zealand endemic genus Lamellomorpha in the family Vulcanellidae (Porifera, Demospongiae, Tetractinellida), with the description of three new species, pp. 1-25 in European Journal of Taxonomy 506 on pages 5-10, DOI: 10.5852/ejt.2019.506, http://zenodo.org/record/261295
Compte rendu de Bergquist (U.), Damascelli (D.), Frimston (R.), Lagarde (P.), Odersky (F.), Reinhartz (B.) Commentaire du règlement européen sur les successions
No full textBergquist (U.), Damascelli (D.), Frimston (R.), Lagarde (P.), Odersky (F.), Reinhartz (B.). – Commentaire du règlement européen sur les successions, Dalloz, Paris, 2015. – 320 p. – ISBN : 9782247141456International audienc
FIG. 4. — Semitaspongia glebosa n in Two new genera and five new species of the "Cacospongia" group (Porifera, Demospongiae, Dictyoceratida)
No full textFIG. 4. — Semitaspongia glebosa n. sp.; A, holotype, NMNZ POR457 (= SDCC/NZ081); B, paratype, SDCC/NZ080; C, paratype, SDCC/NZ078, fibre skeleton; D, paratype, SDCC/NZ078, detail of fibre skeleton; E, holotype, NMNZ POR457 (= SDCC/NZ081), with some unidentified structures at the surface of the sponge, which superficially resemble fibres; F, holotype, NMNZ POR457 (= SDCC/NZ081), histological detail. Scale bars: C-E, 500 µm; F, 250 µm.Published as part of de, Steve & Bergquist, Patricia R., 2000, Two new genera and five new species of the "Cacospongia" group (Porifera, Demospongiae, Dictyoceratida), pp. 383-400 in Zoosystema 22 (2) on page 392, DOI: 10.5281/zenodo.539299
Comparing risk adjustment estimation methods under data availability constraints
No full textThe Italian National Healthcare Service relies on per capita allocation for healthcare funds, despite having a highly detailed and wide range of data to potentially build a complex risk-adjustment formula. However, heterogeneity in data availability limits the development of a national model. This paper implements and ealuates machine learning (ML) and standard risk-adjustment models on different data scenarios that a Region or Country may face, to optimize information with the most predictive model. We show that ML achieves a small but generally statistically insignificant improvement of adjusted R2 and mean squared error with fine data granularity compared to linear regression, while in coarse granularity and poor range of variables scenario no differences were observed. The advantage of ML algorithms is greater in the coarse granularity and fair/rich range of variables set and limited with fine granularity scenarios. The inclusion of detailed morbidity- and pharmacy-based adjustors generally increases fit, although the trade-off of creating adverse economic incentives must be considered
FIG. 1. — A in Two new genera and five new species of the "Cacospongia" group (Porifera, Demospongiae, Dictyoceratida)
No full textFIG. 1. — A, Cacospongia mollior (ex-Topsent collection, Strasbourg), fibre skeleton; B, Cacospongia mollior, showing histological detail; C, Cacospongia serta, holotype, BMNH 1886.8.27.166 (fragment = SDCC/NZ076), fibre skeleton; D, Cacospongia serta holotype, BMNH 1886.8.27.166 (fragment = SDCC/NZ076), detail of fibre skeleton; E, Scalarispongia scalaris (ex-Topsent collection, Strasbourg), fibre skeleton; F, Scalarispongia scalaris, showing histological detail. Scale bars: 500 µm.Published as part of de, Steve & Bergquist, Patricia R., 2000, Two new genera and five new species of the "Cacospongia" group (Porifera, Demospongiae, Dictyoceratida), pp. 383-400 in Zoosystema 22 (2) on page 386, DOI: 10.5281/zenodo.539299
Replication functions of the F plasmid of Escherichia coli
No full textFull text is available to authenticated members of The University of Auckland only.The work in this thesis concerns the replication of the F plasmid of Escherichia coli and is addressed to the question: how many plasmid genes are involved in replication and partition of F, and what are their function? The techniques principally employed were the cloning of restriction fragments containing parts of the F genome, and transposition mutagenesis of mini-F derivatives. The results obtained can be summarised as follows:
(1) The F plasmid contains a second, previously undiscovered, replicon which is located within a 3 kilobase segment of EcoRI fragment f7. The f7 replicon was shown to take over replication of the intact F plasmid when the primary (f5) replication system was inhibited. Replication of f7 plasmids was found to be partly defective in E. coli, especially in rich media.
(2) Several temperature-sensitive mutations of F were mapped within the essential replication region (F43.8-47.0kb) of -EcoRI fragment f5. The isolation of temperature-sensitive mini-plasmids should greatly facilitate study of the nature of these lesions.
(3) A mini-F plasmid was obtained which constitutes the smallest F derivative so far reported. This plasmid is deleted for the two known origins of replication, oriV and oriS, which suggests the existence of a third origin of replication within the f5 fragment.
(4) A locus required for stability of f5 mini-plasmids was mapped to the F46.35-49.25kb region. Two possible functions are suggested for this locus.
(5) The part of f5 that is responsible for the incompatibility reaction shown between IncFI group plasmids was found to map in the F47.6-49.25kb region. Therefore this area constitutes a third incompatibility region of F, distinct from the incA and incB regions defined by Kahn et al. (1978)
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Essential replication functions of the F plasmid
No full textThe work in this thesis concerns the analysis of essential replication genes of the F plasmid of Escherichia coli. The techniques principally employed were the cloning of restriction fragments, transposition mutagenesis, maxicell analysis of proteins, and DNA sequencing. This work led to the following findings. 1. A 1.15kb region of f5 was shown to contain all the functions required for initiation of F replication. Insertions of a transposon within this 1.15kb region abolished mini-F replication. 2. A mini-F plasmid was constructed which constitutes the smallest F derivative so far reported. This plasmid has an elevated copy number, as a result of deletion of the incC region. 3. At least two proteins specified by the essential F replication region were identified in maxicells. The promoter fragments of two mini-F genes were fused to a β-qalactosidase gene and demonstrated to have low but significant in vivo activities
Replication from the primary origin of the mini-F plasmid
No full textFull text is available to authenticated members of The University of Auckland only.The work in this thesis concerns the analysis of essential functions required for replication initiation from origin -1 of the mini-F replicon of F plasmid. The techniques principally employed were the cloning of restriction fragments, DNA sequencing and sequence analyses, assays for replicative ability in DNA polymerase 1 - dependent hosts, protein purification techniques and protein – DNA binding. This work led to the following findings -
1. A 198 base pair region of mini-F contains the sequence required for initiation of replication from origin 1 in a DNA polymerase 1 -dependent host.
2. The mini-F encoded proteins C, G and E are required for replication from this origin.
3. The C protein is a 38,739 dalton protein which binds in vitro to two DNA regions - one contains the operator of the C gene and the other region contains the minimal origin sequence
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