9 research outputs found
Anti-inflammatory effect of Adelia ricinella L. aerial parts
No full textObjective
To investigate the main chemical components and the anti-inflammatory activity of extracts of Adelia ricinella L. aerial parts.
Methods
Three extracts obtained by soxhlet extraction and ethanol/water mixtures were evaluated in their chemical composition by UPLC-DAD-MS/MS. The in vitro anti-inflammatory activity of the prepared extracts was assessed through three different assays: COX-1 and COX-2 enzymatic inhibition, cell-based COX assays on RAW264.7 macrophages (ATCC) measuring the COX-2 protein expression by Western blot and the measurement of the PGE2 concentration in the supernatants of the culture medium. Also was determinate the effect of the three extracts on the RAW 264.7 cell viability.
Key findings
Few differences in the phytochemical profile were found between the three prepared extracts, identifying a blend of thirteen flavonoids derived from luteolin and apigenin, with orientin as main constituent. Plant extracts (alcoholic and aqueous) did not affect the macrophage cell viability (IC50 > 256 μg/ml) and significantly reduced COX-1 and COX-2 enzyme activities. Additionally, COX-2 expression and PGE2 release were suppressed after 24 h of LPS stimulation and treatment with plant extracts (8–64 μg/ml).
Conclusions
A. ricinella extracts showed the ability to reduce the inflammatory effect exerted by LPS in murine macrophages. However, further studies should confirm their anti-inflammatory activity
Abstinencia espontánea en ratones tratados con Mimosa pudica
No full textLa planta Mimosa pudica (moriviví, como comúnmente se le conoce), ha sido empleada tradicionalmente como anestésico, antidepresivo, sedante, antialopécico, entre otras propiedades farmacológicas; sin embargo, en los últimos años su consumo se ha convertido en excesivo. Con el objetivo de conocer el potencial adictivo de esta planta se desarrolló un ensayo de abstinencia espontánea en ratones de la línea NMRI, llevado a cabo durante 20 días, para lo cual se administró una dosis de 1 000 mg/kg de peso de la decocción de la planta 2 veces al día, por vía oral. Como control negativo y positivo se emplearon agua y morfina (32 mg/kg de peso por vía intraperitoneal, respectivamente). Finalmente se comprobó que dicha decocción mostró signos de dependencia física y los animales en estudio manifestaron disminución del peso corporal y aumento de la temperatura rectal con respecto al control negativo, una vez suspendida la administración. Por su parte, estos signos fueron más significativos en el control positivo, de manera que se puede plantear que la decocción de la planta Mimosa pudica a la dosis empleada evidenció signos de dependencia física
Eritrocitos humanos como biomodelo farmacológico de citoprotección antioxidante para la evaluación de nuevas alternativas terapéuticas en pacientes con COVID-19
No full textIntroduction: The oxidative stress can affect the biological membranes of different cellular types in the organism, which has been evidenced in the damages to the tissues and organs of patients with COVID-19, reason why the recent investigations are related to the search of cytoprotector and antioxidant drugs that minimize these damages. Objective: To evaluate the human erythrocytes as pharmacological biomodel of antioxidant cytoprotection. Methods: The cytotoxicity pattern was evaluated in erythrocytes induced by peroxide of hydrogen and the system of diagnosis proposed was valued in a cytoprotection assay in erythrocytes, with the use of ascorbic acid as reference substance. Results: For the concentration of erythrocytes used a cytotoxicity model was achieved to the concentration of 10 mM of peroxide at 30 minutes of incubation. The substance of reference used didn't show cytotoxicity signs in the hemolysis test. In the cytoprotection assay a pharmacological effect of the referent was evidenced, with a value of the cytoprotection index of 12.71 µg/mL. The study of optic microscopy showed severe morphological damages in the erythrocytes treated with peroxide of spherocytes, echinocytes and spheroechinocytes type that significantly diminished in presence of this reference substance. Conclusions: The proposed pharmacological biomodel can be used in the evaluation of new therapeutic alternatives with antioxidant cytoprotector properties for the treatment of patients with COVID-19. Introducción: El estrés oxidativo puede afectar las membranas biológicas de diferentes tipos celulares en el organismo, lo cual se ha evidenciado en los daños a los tejidos y órganos de pacientes con COVID-19, por lo cual las investigaciones recientes están relacionadas con la búsqueda de fármacos citoprotectores y antioxidantes que minimicen estos daños.Objetivo: Evaluar los eritrocitos humanos como biomodelo farmacológico de citoprotección antioxidante.Métodos: Se evaluó el modelo de citotoxicidad en eritrocitos inducido por peróxido de hidrógeno y se valoró el sistema de diagnóstico propuesto en un ensayo de citoprotección en eritrocitos, con el empleo del ácido ascórbico como sustancia de referencia.Resultados: Para la concentración de eritrocitos utilizada se logró un modelo de citotoxicidad a la concentración de 10 mM de peróxido a los 30 minutos de incubación. La sustancia de referencia empleada no mostró signos de citotoxicidad en el test de hemólisis. En el ensayo de citoprotección se evidenció un efecto farmacológico del referente, con un valor del índice de citoprotección de 12,71 µg/mL. El estudio de microscopía óptica mostró daños morfológicos severos en los eritrocitos tratados con peróxidode tipo esferocitos, equinocitos y esferoequinocitos, que disminuyeron significativamente en presencia de dicha sustancia de referencia.Conclusiones: El biomodelo farmacológico propuesto puede ser empleado en la evaluación de nuevas alternativas terapéuticas con propiedades citoprotectoras antioxidantes para el tratamiento de pacientes con COVID-19
Estudio etnofarmacológico de plantas medicinales con actividad sobre el Sistema Nervioso Central, en la región oriental de Cuba
No full textIn the eastern region of Cuba, there is a traditional use of medicinal plants for various pharmacological purposes, although an inventory of these plant species necessary for the future search for new phytomedicines does not exist in all cases. This is the case for those used to treat central nervous system (CNS) disorders. To evaluate the traditional knowledge in two regions of Eastern Cuba: Santiago de Cuba and Holguín, regarding the use of medicinal plants with sedative, hypnotic, and anxiolytic effects, an ethnopharmacological study was conducted in two health areas of the main municipalities of these two provinces. A total of 400 people were surveyed, performing the socio-demographic characterization of the evaluated population as well as the qualitative characterization and evaluation of quantitative ethnopharmacological indicators such as the use value index and the significant use level of the medicinal plants used. The surveyed population in both provinces was predominantly female and over 60 years old, with a high level of preparation and professional training. A total of 27 plants were reported in the interviews conducted among the 1637 citations obtained, with similar results between the two provinces. The highest number of citations in both regions corresponded to plants used as sedatives, followed by hypnotics, and finally anxiolytics. Among the most used parts of these plants, the use of leaves prepared in the form of infusions predominated. Of the 8 plant species with the highest acceptance by the population, the plants P. neochilus, J. pectoralis, L. inermis, and P. incarnata obtained the best values for the evaluated ethnopharmacological indicators.En la región oriental de Cuba existe un uso tradicional de plantas medicinales con diferentes fines farmacológicos, no existiendo en todos los casos el inventario de estas especies vegetales necesario para la futura búsqueda de nuevos fitomedicamentos, tal es el caso de las que se usan para tratar afecciones del sistema nervioso central. Con el objetivo de evaluar el conocimiento tradicional de dos regiones del Oriente de Cuba: Santiago de Cuba y Holguín sobre el empleo de plantas medicinales con efectos sedantes, hipnóticos y ansiolíticos, se realizó un estudio etnofarmacológico en dos áreas de salud de los principales municipios de estas dos provincias. Se encuestaron un total de 400 personas, realizando la caracterización socio-demográfica de la población evaluada, así como la caracterización cualitativa y evaluación de los indicadores etnofarmacológicos cuantitativos índice de valor de uso y el nivel de uso significativo de las plantas medicinales empleadas. La población encuestada de ambas provincias fue predominantemente del sexo femenino y en edades superiores a los 60 años, con un alto nivel de preparación y formación profesional. Se refirieron en las entrevistas realizadas un total de 27 plantas entre las 1637 citaciones obtenidas, con resultados similares entre ambas provincias. La mayor cantidad de citaciones en ambas regiones correspondieron a las plantas empleadas como sedantes, seguido de las hipnóticas y finalmente las ansiolíticas. Dentro de las partes más empleadas de estas plantas predominó el uso de las hojas, preparadas en forma de infusión. De las 8 especies vegetales con mayor aceptación por parte de la población, las plantas P. neochilus, J. pectoralis, L. inermis y P. incarnata obtuvieron los mejores valores de indicadores etnofarmacológicos evaluados
Farmacovigilância da carbamazepina no tratamento de doenças do Sistema Nervoso Central
No full textAnticonvulsants are crucial for treating neurological diseases, necessitating close pharmacovigilance due to their potential risks. For this reason, a prospective descriptive study was conducted to characterize drug-related adverse reactions associated with the use of carbamazepine in the health area of the "Ramon Lopez Peña" Polyclinic, covering the period from July to December 2022. The sample consisted of 35 patients, characterized based on demographic, clinical, and pharmacotherapeutic variables. The detected drug-related adverse events were classified according to causality, severity, and frequency of occurrence. The results showed that the age group of 40-75 years (45.7%) predominated, along with female gender, mixed ethnicity, pre-university education level, and 54.2% did not have any unhealthy habits. The most common diagnosis was epilepsy (68.4%) and among the associated diseases, hypertension was prevalent. The most consumed medications were captopril, hydrochlorothiazide, paracetamol, and metamizole (dipirona). Drug interactions were detected, and 73 adverse reactions occurred, with the nervous system being the most affected at 56.0%. Possible adverse reactions (42.5%), frequent (49.4%), and mild (58.9%) predominated. This study underscores the importance of continuous and effective monitoring in pharmacovigilance to enhance patient safety under carbamazepine treatment, thereby promoting a more rational use of this medication.Los anticonvulsivantes son cruciales para el tratamiento de enfermedades neurológicas y requieren una estrecha farmacovigilancia debido a sus riesgos potenciales. Por este motivo se realizó un estudio descriptivo prospectivo con el objetivo de caracterizar lasreacciones adversas medicamentosas asociadas al consumo de la carbamazepina en el área de salud del Policlínico" Ramón López Peña”, en el período comprendido de julio a diciembre del 2022. La muestra consistió en 35 pacientes, caracterizados según variables demográficas, clínicas y farmacoterapéuticas. Las reacciones adversas medicamentosas detectadas se clasificaron según causalidad, severidad y frecuencia de aparición. Los resultados mostraron que predominó el grupo de edad de 40-75 años (45,7%),el sexo femenino, los mestizos, el nivel de escolaridad pre-universitario y el 54,2% no presentó ningún hábito no saludable. El diagnóstico predominante fue epilepsia 68,4% y entre las enfermedades asociadas la hipertensión arterial. Los medicamentos más consumidos fueron el captopril, hidroclorotiazida, paracetamol y metamizol (dipirona). Se detectaron interacciones medicamentosas y 73 reacciones adversas siendo el sistema nervioso el más afectado con un 56,0 %. Predominaron las RAM posibles 42,5 %, las frecuentes 49,4 % y las leves 58,9 %. Este estudio destaca la importancia de una vigilancia continua y efectiva en farmacovigilancia para mejorar la seguridad del paciente bajo tratamiento con carbamazepina, promoviendo así un uso más racional de este medicamento.Os anticonvulsivantes são cruciais no tratamento de doenças neurológicas, necessitando de farmacovigilância rigorosa devido aos seus potenciais riscos. Por esse motivo, foi realizado um estudo descritivo prospectivo para caracterizar as reações adversas medicamentosas associadas ao uso de carbamazepina na área da saúde da Policlínica Ramón Lopez Peña, abrangendo o período de julho a dezembro de 2022. A amostra foi composta por 35 pacientes, caracterizados com base em variáveis demográficas, clínicas e farmacoterapêuticas. Os eventos adversos medicamentosos detectados foram classificados de acordo com causalidade, gravidade e frequência de ocorrência. Os resultados mostraram que a faixa etária de 40 a 75 anos (45,7%) predominou, juntamente com o sexo feminino, etnia mista, nível de escolaridade pré-universitário e 54,2% não apresentavam hábitos não saudáveis. O diagnóstico mais comum foi epilepsia (68,4%) e, entre as doenças associadas, a hipertensão arterial foi prevalente. Os medicamentos mais consumidos foram captopril, hidroclorotiazida, paracetamol e metamizol (dipirona). Foram detectadas interações medicamentosas, ocorrendo 73 reações adversas, sendo o sistema nervoso o mais afetado, com 56,0%. Predominaram reações adversas possíveis (42,5%), frequentes (49,4%) e leves (58,9%). Este estudo ressalta a importância do monitoramento contínuo e eficaz em farmacovigilância para aumentar a segurança do paciente em tratamento com carbamazepina, promovendo, assim, o uso mais racional deste medicamento
Identificación y prevención de riesgos de origen químico en centros laborales de Santiago de Cuba
No full textIn the TOXMED Occupational Health Service, we sought to identify current chemical risks and analyze their association with work conditions in 11 work centers. Information was obtained on the chemical composition of the substances, their uses, associated hazards and toxicities, duration of exposure, direct and indirect exposures to the chemical substances, use of personal protective equipment, occupational hygiene measures, the presence of natural or artificial ventilation and other technical characteristics of the productive process. The most common chemicals were hydrocarbons, metals,
acids, and alkalis; there was evidence of widespread incorrect use of petroleum derivatives. Carcinogenic substances, inhalation hazards, corrosives, irritants, oxidizers, flammable compounds and a mutagen were identified. Regarding work conditions, the absence of personal protective equipment (41.84%) was most commonly reported, followed by non-compliance with occupational hygiene measures (28,57%), and, lastly, inadequate ventilation and emissions of chemical substances (16.32%). This assessment of occupational risk allowed the evaluation of chemical hazards, and a qualitative assessment of potential risk by job, previously not evaluated from a toxicological perspective, as well as compliance with recommended preventive practices to decrease the negative impact of chemical exposures on worker health.En el Servicio de Salud Ocupacional de TOXIMED, nos propusimos identificar los riesgos químicos presentes y analizar su interrelación con las condiciones de trabajo en once centros laborales. Se obtuvo información de la composición química de las sustancias, sus usos, peligrosidad y toxicidad, tiempo de exposición, la exposición directa e indirecta a las sustancias químicas, la protección personal, la higiene laboral, la presencia de ventilación natural o artificial y otras características técnicas del proceso productivo. Predominaron los hidrocarburos, los metales, los ácidos, y las sustancias básicas, hallándose un uso erróneo generalizado de derivados del petróleo. Se encontraron sustancias carcinogénicas, tóxicas por inhalación, corrosivas, irritantes, oxidantes, inflamables y una mutagénica. Con respecto a las condiciones laborales hubo una supremacía en la ausencia del equipamiento de protección individual (41.84%), luego el incumplimiento de las medidas de higiene (28,57%), y por último se presentó el problema de la ventilación y el escape de sustancias químicas (16.32%). La Asesoría de Riesgo Laboral permitió considerar la peligrosidad de las sustancias empleadas y valorar cualitativamente los riesgos potenciales por puestos de trabajo, no antes valorado en estas entidades con un enfoque toxicológico, y el cumplimiento de las medidas recomendadas para el control de los riesgos estuvieron encaminadas a disminuir el impacto negativo de los riesgos de origen químico en la salud del trabajador
Acute Toxicity and Dermal and Eye Irritation of the Aqueous and Hydroalcoholic Extracts of the Seeds of “Zapote” Pouteria mammosa (L.) Cronquist
No full textThe common use of Pouteria mammosa (L.) Cronquist, “Mamey or Zapote,” in food and ethnobotanic medicine shows its low or absent toxicity as fruit extracts prepared from seeds. However, it is essential to conduct security trials to scientifically support their use in drug therapy. This study evaluated the aqueous and hydroalcoholic extract (25%) Acute Oral Toxicity, obtained from the seeds of P. mammosa, in Sprague Dawley rats and dermal and eye irritability in New Zealand rabbits. The 404 and 405 acute dermal and eye irritation/corrosion guidelines were used, as well as the 423 Acute Oral Toxicity guideline, Acute Toxic Class Method of the Organization for Economic Cooperation and Development (OECD). The aqueous extract was located in the following category: not classified as toxic (CTA 5), while hydroalcoholic extract at 25% was classified as dangerous (CTA 4). Both extracts can be used without side reaction that irritates the skin which permitted classification as potentially not irritant. P. mammosa in the two extracts caused mild and reversible eye irritation, and it was classified as slightly irritating
Anti-inflammatory effect of Adelia ricinella L. aerial parts
No full textAbstract: Objective To investigate the main chemical components and the anti-inflammatory activity of extracts of Adelia ricinella L. aerial parts. Methods Three extracts obtained by soxhlet extraction and ethanol/water mixtures were evaluated in their chemical composition by UPLC-DAD-MS/MS. The in vitro anti-inflammatory activity of the prepared extracts was assessed through three different assays: COX-1 and COX-2 enzymatic inhibition, cell-based COX assays on RAW264.7 macrophages (ATCC) measuring the COX-2 protein expression by Western blot and the measurement of the PGE2 concentration in the supernatants of the culture medium. Also was determinate the effect of the three extracts on the RAW 264.7 cell viability. Key findings Few differences in the phytochemical profile were found between the three prepared extracts, identifying a blend of thirteen flavonoids derived from luteolin and apigenin, with orientin as main constituent. Plant extracts (alcoholic and aqueous) did not affect the macrophage cell viability (IC50 > 256 \u3bcg/ml) and significantly reduced COX-1 and COX-2 enzyme activities. Additionally, COX-2 expression and PGE2 release were suppressed after 24 h of LPS stimulation and treatment with plant extracts (8\u201364 \u3bcg/ml). Conclusions A. ricinella extracts showed the ability to reduce the inflammatory effect exerted by LPS in murine macrophages. However, further studies should confirm their anti-inflammatory activity
0003
No full textPalo Alto Times
M'to t-uiaiJ araar raiPAV
11. W. SIMKINS. raoraiBToa.
TERMS j^
Dalltered by Mail or CWrlsr
AdTertlslrif rat** oo applies tion.
TOWN OIUCIAL PAPER
WEATHER REPORT.
The ■aataasas aad mlt-inium ttcrmocirtrr
dutlne tbe past week reiflsierrd bb followa.
Report br U rather Utaioreer J. A, Squire:
■tUXIML-M MIKIMt-U
ri-tday M *
Saturday Si * at)
Bur-day U S3
Monday ou ST
TaestlBy SS 30
Wr.1o*;*tlay St 41
Thursday (April la! S* ta
Ralofall for past woeB. "'■ lose.
Halofall for ibe *«aaoa i. *V lache-,.
Kale-all lo date laat yo*r. lift- laebra.
APRIL 17, 1903
THE OPPORTUNITY AWAITS.
Tbo Time* editor'* letter from Los
Angeles In last week'* issue voiced
tbe policy tbat has made Southern
California famous. That policy bas
beou advertising, - pursiateut, continuous advertising. Tin-, publicity
campaign wus beguu iu 1S8.1 aud biit*
uuvur coasod. Every hamlet,-every f
town, every city, nud Southern Call-1
forum as a whoie, ha* beeu kept In
tho j lubllc^eye at all times aud iu all
possible ways. Tbl* work ha* eo*t
thousands and tbouaauda of dollar*,
but It has brought mllllouti iu return.
Thc i-uin has uot i .--■■ ulune in the
swarms of tourists who come each see,*:
sou and spend monoy with lavish
hand,—the southern valleys bave he-
-&eau **• tilled wltb orchard* aud tbe hill-
sides **.' " dotted with tbe boni*** of tbc
■wealthy p*,'°P-e ■*no eaoie aud were
coot-uered. The elfoct* are cumulative, for every lies- rvldc'l becomes
a strenuous advocate 01 ■*"■ "dopled
locality and helps to sprond If* '"-"fl-
Aiprugressive citizenry make a pros-1
m-t,,i. . community.
Southern California doe* not tlur- j
pas* tbe Coast Couutio* either in;
tHteuic, climatic, or productire adrau-
CULTURE OF SPONGES:
Fish
Commission's Work
tbe Florida Coast.
Off
ENT1EE COMESDI FLY TO BE BAI8ED
In.frau at aa laasorler, the Inln-tl
Itate-a Will in.,,, lit- aa Ripot-tcr of
Spowsea—Alaulalaat Uirr Coal-led
liipi-rluirnirr. la lurrri.ur lllffl-
c-sllle-e.
RALEIGH LEGEND UPSET.
lIlBtnrlaa Oesas Ss-rs Sir Walter
N*«-»r Visits** **nrih Aai.rlra.
"Walter Ralelgb never set foot In
North America. Yet sopertlnadous'ls
the legend connecting bim with Virginia tbnt I doubt 1/ any one. even In
tbe learned assembly. Is not satisfied
tbnt he did."
So B'Hik** Edmund Gos*e, tbe leading
authority on Ell-uibetii.it) literal tin*,
before the Royal Geographical society
s few days ago on tht* tercentenary of
Qii'-'-n Elizabeth's death, write* tbe
New York World's Ixindon t-otrespoml-
ent. Mr. Goase added:
"Hut In spirit be waa tliere. tbrough
good r.nd evil estsle. U1b wbb tin- brain
tbnt planned, tlie persistence that carried out. Uie courage tbnt would never
Considerable progress Is being made
by tlie United States govcrutuont
tlirotigli tbe n-ii i-ommlHNluu In tbe ar-
IIHi-IiiI propagation of sponge growth
off ibe coast of Florida, uml the stale- I relinquish the design, ami the promised
tiu-nt wus made at the commission tbe la,uI v'««nla la bin In history, although
„„ _ , .a . - . . . , be only gnxed at it from tbe fringe* ot
other day that *o suceciwfiil had -•**-' the cloud" -* -• *-*>
culture been tbat within a few years'
aCake Walk s-n.rr.r.ir.i.
The fickle Pnrlslans nre already tired
of tbe cake wnlk nnd ore balling with
enthusiasm n now dunce en lied "the
dnnce of the veil* wiys n «--eoliil Purls
cable dlapnlch to the New- York Her-
imports aid. Girls hove folds of muslin attach*
ed to each aide of the corsage which
The .-\|-.-r-i-:.-i,T- buie been eouduct- 'cok like diaphanous wings. The ends
•■d for aoitit- time past untler tbe dirvc- ,r*? •''*•■■ ll- ,t-*? hands, and the more-
tlon of Dr. II. F. Moore, assistant com- -*■*■■*" "urlng the dance cau*-* llu- ihme.
mlaalouerof fish and fisheries, who 1ms _. t0 uv?_*' to -* &**** t***V* ■»'
tbe United States will produce all tbo j
arrange* rousuiued here, wllh n balance
left over for the ex port trndo. says the
WiiKlittiglnn Pout. At thesjireaent tlm?
the sponge supply u fur Mow tlH- di-
iiiaiitt. uud tbe I'nlted Stulet
most of tbose required.
dcvotetl coitfiltleruble bun) work und
tieiition tu tin* matter. The actual
work or planting sr*\n«tm In ih» -.v-iu-r*
lie rtorliln coast Is now lielug con
l diieted huder tin* Mii|M-r* l-loli of Cap-
j lulu Jniues A. Smith of the Flab Hawk.
i This rssssi now iu Florida, will return
to WaabltiKion at ll.-■ ctota* of lis present erulae. about April 13.Villi n large
number of -.jtveliueiiH und (-oiiNlderable
dntn. The work Mccumpllsh.-d by the
experts' of the Qsh bureau haa been
uiili|tie, but suifi-w itppiirviitly bus
crowned the effort.*, uf tbe commission.
uud twu ripuiurra will be made to grow
*v lie re only one coultl find todglug place ,
before.
Tlie Bhonge* U*k*d
ore tlH shcepshe;
wings. Tbe Blow, majestic tlaiic*- linn
jraetlcnily dethroned thc negro Bpe-
:1«IU-.
CUARDIAN'S SALE OF REAL
PROPERTY.
In the Superior Court of the City
nnd County of Snn Francisco,
State of California.
msw ..i ,'tsr/
ised lu ibe PM'*>(ration *^Ri.Sft p**
it-ad variety, me most' fEmV_rJ_c
«tr....... .,_ siianco of the
In the matter uf the Peraon aud tbe
e*t*teof Mary Kunic-.-Clark, an Incom-
" 2ae-l. Department Ten.
is herebv given that In pur-
in lbe world.mid one which -ill* pru.- Frnncl*eo, Stat*, of California; De|-arl.
tli-nlly every i-onuuercliil ne.ero.Hy. Tbe mont Ton thereof, made uu the -.Till
uu-tliiid used lu plaining tlieiii on tin* Day of Muivh. IW3, In the maltor of
ComJ ***•% nnd roek's of tlie tropical' the person and estate of Mary Eunlw
water* Of lbe Florida coast hns 1-.-.-U Clark, an lncom|>ctcnt, lho undersigned
evolved afu-r many failures by Ur. i-uardlanof the ^nson and lho estate
Mi-ii,-. Who li.i- nt I.i-i bit QpOfl a way
out of the ditiiculti.** wbich filled It.
of aald Mary Kuni.-.- Clark, an Incompetent, will sell at PRIVATE SALE
to tbe hlgheM ami hest ... .;.;.■•■ for cash.
tages. IU one anperior poiut has j I'm'1 oT the coin mission when ll Omt ,w0j,i oolsi or the Cultod Stat*.-*, on Dr
beoii lu It* people,- it people truly
public-s|iiritcd, loyitl to tbeir own
town and tbelr section of .the State.
Local jealousies are nut tolerated and
dhdoyslty would be met with ostracism. Tbat Is tbe spirit wbich should
exlat here, a spirit which U fortunately becoming moie lu evideuce
i*aeh year. When
fully awake to their pontiibillties,
when they realize that Inveatmcuts lu
-idrortiMlug are ceitaiu to Imi ruturued
to tbem a buudred fuld, thuu will the
undertook '.be work. after the .killi Day of April,
"In pluming tbe npoiu.-ea." wild Dr.' lho right, title-, inteivnt. cltiliii, prop*
Moure, "we have now founil u ■cbwttT^tffly ami estate of lbs »a d Mary Eunice
wiiiei, Im- pnivvd Hntlsfiiciory. The Clark, an Ineompetenl, Iu ihiuto corlaln
*|H>nK.<* are eat Into .mull ple.-es from °J'*- t*«** mll£BtrwK °LI"nd &B&
' ■ ii . _ *r, ^* lying »i»l being in the lown of Palo
one to two luetic in dlameirr. These ^ CouDt J^^ _ StaU) of
small fntKiueut* of the living *|K»nge. California, and more parltcularlly do-
wlilch are ilark in rotor, wllh lbs pore* acri***! a* follows, to-wlt:
noticed in tbe cured s|-otu:e of com- Commencing at a point ou the north*
l>eople arof uioroe filled by Hmhy inntter. nn* firm- westerly llnt-uf Atldison Avoniio distant
j ly fnmeiutl to pieces tif coral, rock nnd one luiuilreit tidy (l.'-U'fwt from tho In-
i terra colt* brick nnd dropped over- terseo-lon of lho said nu,-thwoatui-ly
: bounl. tin- work being done on board Hue of A.-lUon Av.u.uo with lho norlli-
., -. .. ^.. iaai . ... i easlsrly lino of \\ avot-U'-atriHii; ihonos,
tlie flail eommiHSion vessel. nurth,'u*U.rlv alons; safd nurtheasterlj
-Tbln aluminium- wire Is used to do -lr.0 o( Addl*lou Avenue, one hundred
Santa Clara valley become n- well this, nnd In bitting u|»n thta wire we ,ioo) fr-ot: ihmce at ri*fht angles and
kuowu and as popular a* la Southern solved tbe dlfltculty. Tbe piece* of parallel wltb Waverly Street north*
B|miiKe usetl have one outer skin inlnct. westerly two hundred (200) feci; thence
with the oilier t-tu-e*. raw. The luller. at right angle* and parallel with Add!-
however, quickly heal. The aluminium -"on Avenue southwesterly one hundred
wire will, of course, pierce tbe Sponge (--*•. feolj llienco at right SOffloS and
und for.,, u amnlllKircorltoli'tbroui-b =^ *££&(£&. Ktfc
tbem. The win.- corrode*. II this Is vulol Jt eomwtaw_*n, ihi samo being
nu ndvanlage. for it gradually wears part of lul two I2) block fifly-seren (37).
awny. leaving lbe sponge free of auy ALSO, commencing at a point on tho
foreign substance. nortbesslerly line of Waverly StMt-l
"Mont of ihe dim nil tie* attending dUtant one hundred ten (110) feet
the culture have been met wltb lu ai- northwesterly from the Intersection of
tempting lo Und someibing lo Mud the tbo suld northeasterly line of WmriJT
sponges ,o the rocks whlcb would last **__>&!_.!___^
long enough for the sponges to uttactl
themselves nnturully to the new bed.
Tbe nlumlulunt w-lrv doe* Ittla. Wood,
string, copper oud iron w-lre unit •/art
ou* other *ub*tancc* we found were at
tacked by tbe salt water and animal easterly
life and rendered uselt***.
' "Tbo experiments are being conducted nt Itliicnynt- bay. Anclote key* nud
off Key West. Tbe dealers nr«- partly „„„,;,,,,.,,.L ,*.„,-.-,,,- bolng iiarl of lot
resj-onsllile for lbe poor eomlllloii of twi,(;.) block llflv ■ seven (Si),
tbe donieailc qwnte mnrket '■',
PROMOTION WORK.
The California Promotion Commlt-
Ue 1* using every effort to bring people to tbl* State and la meeting wltb
marked suocss*. A report Issued by
tbe committee 1* aummarizeil below:
loqulris* regarding California during Mut.'I. show a couslderabl* lu*
c res no over those of previous months,
being 4,001 a* against 3,Olio lu February. This is a matter of gratification to the committee, prorlug tbu
efficiency of the various plaua of advertising California golug oh throughout the Eastern States and abroad.
Tbe uuniber of letters sent out by
the Committee 1* constantly ou tbe
increase, 8,307 letters having been
mailed during-March, ns against 6,075
iu Kebrunry.
A large Increase Is also to be uoted
In tbo muni -I of addresses of psrtlt-* '.
lotersjatsd lu California tbat bave \
been sent to the Committee.
Two of the six representative!, who
!,:i--- been iu thu £aet giving lectures'
have returned, uud rejiort excellent j
results; olhor mon have vol uu toured
tholr services for like purpose, to take
tbe pluco of tbose returning, aud tbe
work Is being carried on vigorously
throughout tbe Eastero States.
Tin* map of California Is now roady
for distribution, aud Ib up-to-date iu
every iiarlicular. Tbis map will bo
of peculiar value to tbo homo**cuker,
ouo feature being un ornnigotnout of
the prluclpnl products of the Stato lu
alpha be tlcitl order aud tthuwlug tbe
countle* where tbey are produced.
Large uumhvr* of people are coin-
In Into the Slate, attracted by the ad-
vertiliug being done and by the low'
rates of trniiHiKirtntjou now lu force,
the record showing u total of l!0,03!i
since February 15th, Il is also u
noticeable fact that there are more
tourists lu Sau rfanclncu tlmu lu
previous year*.
Letter* are being eoustautly referred lo tho Committee for atteuliou
by business mon uud State author!-
ticf, und the Coininltteo Is Istiiig directly addressed by people not only
lu the L'ulted Stntvs who uro Intercut-
lu California, but by -H-oplu
throughout tbu world. Tbo work
a* assumed large pro|*ortioua, and,
*tb tbe fotitiuue-J i'-.-it-..-t.iti.-ii uf
ul!. will bring results of permanent
benefit.
Test* hIiuw that It ret|uire* tun
miutttui* of iii-tual boiling to kill, thu
•asv germs In milk ur wnter.
| The Dissolution of Partnership Sale *
Ijll OK niA/ru ,v COMPANY
I to Is a Great Success
£_g_r_.
Showing over 300 per oent h
WHY? WHY?
Because Prices are Away Down
and the Stock Up-to-date j&
ffi
SS
ir
o our apologies to those we are unable to
on ue.-in,i,i of the rush
alt upon
Special Bargains every day for Balance of Month
'I'M MI-aiT
FRAZER £ CD.
s-i,- . ..ur. to Ferffuaoa .'. Co. si..i,r..s,i li.iii.iin,;. l*alo Alto
tiiV
©
STRONER dt BACKUS
PAINTERS
Phone lied I2-"'
P. O. Boa 92t)
Wall r-aaer. Ilar-lwarc. Wlntlow Olaea
faints nrttl Oils, fnlntimi. Whilenlng Tint
inR, i'lniti aad Ih-t-iiratlTe Taper HuuK-it*.
NOTICK-— In order to give thc bes
service to our custotners, wr have
decided to adopt tbe following
rule* After March 14, 1903, a-ll
orders for Ice Cream to be packed
in ice or delivered on Sunday
must be giveu hy Saturday evening.
(Signed) A. VL i'aaphrcys,
lvrtiest Wilson. 4t
T0URI5T EXCURSION PARTIES
TO THE EAST
Over tbe 5cenlc Line of the World
If you are going Hast and want to
save unmet, yet travel wlib pleasure
aod comfort, ft will pay vou to Invest!-
irate our dslly Personally Conducted
Tourist Excursions These parties are
Id charge of a Manager, who accompanies lho car through to Chicago and
lho Atlantic Coast and gives his per-
•..mill attention tu tho welfare ot each
pasM-ngur in his cbargu. Tbuschetlulus
are srranged so a* to pan* tbrough the
world-famed scenery on tho Denver A
1:1.. Grande Railway by dayllgb I.
Thoao desiring may also have tbe pmll-
c-go of a -.top-over In Salt Lake City.
Lot us k00w where you are going aod
wo will be glad togivorou full Information abou* your trip, tbo loweat
rate*} Of fur., and sonil you free of charge
some ha.i.deonu'lv llluatratod books uf
travel. J, D. MANSFIELD,
(ionem. Agent,
'.'.'■a Market St.. San Praoci-aco, Cal.
California.
Palo Alto is still patiently awaiting for some enterprising jierson to
build a tourist hotel.
Very few of the Palo Alto homes
ar*. fortunately, enclosed witb fences,
lu some in-ill-.'-- - there are hedge* often no high tbat on.- cauuot see over
them. Why not either remove these
hedge* or cut them low, »o that the
llorul beauty of tho ground* may be
tt - Our towu cannot bo made too
attractive.
One day we bear that tbe Sbam<
rock lit is tbe fastest yacht yet built
aud is sure to win the cup. Ou another day sbe Is declared to he h dis-
npolutmout und In not fan! ut nil,
Wo will ull know nn ue nil.mt the merits of Sir Thomas Upton'* new craft
aud the Americau cup defender after
lbe race ba* been sailed.
Juhu D. Rockefeller uud party
-Vlulled Stuuford on Saturday. Mr.
Itoekefellur Im much Intert-nteil in
uiiiver-ity work nud would luitorully
desiro to i*-.** the urebll.-etiirnl ideal
of a uuivendty as It estate here. He
may now ta expected to donate au-
other million or so !<■ Ihe Chicago
Uuiveraity ill the bono of keeping tbnt
Institution nbreiift of Sltiufonl.
Addlsiou Avonuo; ihenco northwesterly
along said northeasterly lino of Wavorly
Street olghlyliv*- (So) fool; tbonce al
right aiiglua'ituit parallel with Addlsloii
Avenuo nurtbbaittorly ono hundred '.■'■■■:■
■ lfOi fe-i: ilieiuv at right angle* aoulh-
iriy and parallel with Waverly
Street elghly-llve |85J feet: thence al
rigOt angU-s southwesterly and parallel
with Addison Avenuo ono hundt-ud and
ttfty(l.'iO) foot to tho point of com-
I-tlrib- ALSO, beginning at lho jwlnl of ln-
1 tnke ull ■pollers eiiught on their torsection o( tho northcnsiorly lino of
large ami amalt. on tbe principle Waverly Street with tbe soutbeaslerly
line of Channlng Avenpe, ruunlog
tbenco nortbt-asierly slosg tbeaoutb-
easterly Hoe of Cbanolng Avonuo one
buudml aud (Ifly < 160) fewt; them*.- at
else
IV ill.
tbnt If they don't some oue
and tbe result Is Ibal tbe Indoatry Is
ruined. The commission la doing thl*
work only lo show the>**Mfier way to •r,^"™^™asoulhu^t^auiV'imValM j
promote the **ponfie Imlunlry. mid the w(lb Wm-erly Street ono huiiiliiKl ami
work la nliulhir to that done In tin*
propagnfioti and COlIUItVOT the oyHter,
Ihlny-tlvo (i:).r>) fool; thonco at right j
ingles south westerly nml parallel with
We will demonstrate lb*' method to the Chaiiuing Aveuuu one hundred unit
denier* and then let"private Mtaiprltt BftJfJltTOjfiot to the northeusU'rly lino
of Wi
_. iVavorly Street; thence along said J
at norttiea-ileriy line .of Wai-erh Suvel
northwrstcrly one lnin.Ir.-J and Ihlrly-
:'.-..- (135) *.■■-. to lbe |roint of com-
nieiieenieiit, tin* same being part of lot;
one (11, block ttfly-scven (57).
it«fi'i-ein\' iK'lug tioroby outdo for u j
j murtit-oui]iIoted-.-Mii-iplion of wild 111-eiii- ,
Iln-srf \-alert, as l.n*lrr l~-|„tvera. IhCk til tllO IllUpof nail) Ulliveculty I'ltl-k, j
"We hill'.* Ihe siitne- llowert* llilu Ba** "OW BalfJ Alio, UD tihi ill lho ulliiiulf ;
ter that we bad hut." MlM ii lending *J» Ctjuntj '\™"*or of said !*anta -
for Irrigation uses in thl* valley has a. i!..-|*i nt tlie national capital to a **-?
The cumpany that pr<i|iti-es lu briug
water from the Mmiut lluuiillou rungo
take tin- matter
"Tliere la every rvn*on lo lield
tbla will ta done and Ibal In n compar-
ntlvety abort time nil lh** sponges neetl-
ed in the United State* will ta nilse-J
tn the tvatera of Florliltl."
wide Held of useful 11 van before It
An abuudaut nupply of water would j «cc-*tion of
uot only vantly lucre*-e the produc-
tlvunt>*-< of the valley, but would have
a direct affect in cmmlm* the foothills
to bt-enmo thickly populated, for
these foothills would liecujno the most
do* I ruble and picture nil tie home section lu California.
..Vi-1.-"".■.". al page *ixlv-n.netli9l tbereof, asahown |
Washington Post reiwrter. "With the by\bl, ^.^ ,,, SaoU Clar* County,
Tbu n-vent election lh Oakland had
as oue of its main t-Hm-iuLgii Issues tbe
acquisition of uiutiicipnl water works,
aud thu new mayor *eetus dutormlue*!
to give the people what they wuut.
Tbe Contra Costa company, which hns
luog supplied Ouklaud with poor water
at high rate*, is much concerned.
Tbe oompauy must either sell It* plant
at a r.-a»i >u a hie price or see tbe city
secure its water supply from some
otbor source, and neither Hlteruatlve
1* to thu liking of that iiiuuopoly.
azaleas, I tlou't ^t-tc of California, In the office* of
know of a single flower tbat ban not ^ the County Recorder of said County.
bean lo popDlar fnvor for (ha lasl t*-n [Ji,ia will lie recailvod for the whole
yean* Aznleas buvo beeu In vogue for or nnrta of tho projierty as above do-
ninny resn but it wns not until Inst ■ *K;rlbod. and any person offering l*> pur-
fall thut floriculturists succeeded lu , chase tho MUM whose hid shall In, a.-
I copied shall, on notice of tho tux-opt-
woaad-t. aj-arr >ari-gof ft» -i»A ,nWo, |h.ioMsd.pMil«,.|, th,
cles lienrlug powers. ltu*> WUl lie _,_._, .v. *v. ............
used very extensively this year
,„. t dersigned,
,nrithe addrc-
witb her atturneya,
hereinafter mentioned, ton!
church decorations and Easter flowers, ^r wnl Q- ibv pUrenTjja money, and
-.-■-ii'-r 111*. itii.-uiti' li n*,a large ntimlM-r j j,a.. xll„ balance on tbe approval aad
of dwarf azalea busbe* can be placed ; conlirmatlon of the sale by said 1'.... 1 ■.
within a limited space, presenting a
very attractive sppeantnee.
Piss Is lladr Cotton", firowtb.
The Hrltialt Cotton Oruwlng iissta'ta*
tion Is -M'Uillng missions to study the
growth of cotton lu different Boll*, to
secuiT the beat seeds *n-l lo distribute
tbese to eolnolal native* Id Egypt, the
Egyptian 8udnn, India, tbe oceanic Islands. Uganda. East Africa and more
particularly In west Africa nt Sierra
Leone, Lagos uud the lower Niger.
Abstract uf title aud uonvuvauc*.* to be
al tho uz-hs-ui-o uf thu purcnasor. All
sales lo bo subject lo the continuation
of lho Court.
MRS. GEORGE HOLDING,
Guardian of tbe Person and
Estate of Mary Eunice Clark,
an lucompeteDt.
Dated April nth. IMS-
PULLER A: CHAPPEL,
Attorneys for Guardian.
Rooms 8-0 Third Floor, Mills IJulld-
Ing, Rush and Montgomery Street*,
San Francisco, Cal.
Indian
Baskets
We have now in Block a largt;
variety of genuine Indian baskets made by the St. Lnwrvnce
river Indians at Bombay. New
York, on the Canadian line.
Tbey have a peculiar interest
on this account, hut tht* reason
we put tbem in slock ia la-cause
they urt- very pretty, serviceable
nud cheap. Laundry baskets,
wuste basket--, work baskets,
Hower baskets and others. Take
a look at tbem.
The Flagg Prints
,\Ve have lately received from New York a shipment
of copyrighted pictures suitable for posters or room decoration.' Too many Styles to lie described in detail. A
large numlacr of those cute dog pictures have latelv been
sold.
Fountain Pens
Our ntir-ortnieiit of fountain jk-iis is always kept complete. The Waterman unit Lincoln makes are recom-
mended. Beery hand and ev^rt-p*1!*-*- suited.
SIMKINS BUILDING
Writing Materials
Engraving
After furnishing yon with good |H'iih ami ink, we invite vour attention to our stock of pajH-r and envelopes.
We Veep strictly up to date in the matter of *-tyles and
our large sales in this department guard** against accumulating any old and obsolete stock. Your stationery will
be* correct if we furnish it. Probably one hundred'or
more varieties to choose from.
We make a specialty of printed ami engraved visiting cards, invitations or *.nnouncemcnts.- This is a trade
by itself, and the only safe way is to place your orders
with a dealer wbo has bad manv years experience in
catering to fasbionable demands, r —.. V-a,**"—*~
H. W. SIMKINS .
Bookseller and Stationer
PALO ALTO, CA
