10 research outputs found
‘There’s a lot of freedom you can have with that kind of thing’: vinyl and cassette split releases in the digital age
No full textReleasing music on cassettes and 7-inch singles has been a part of DIYmusic scenes for a long time. As music production, distribution and consumption have been subjected to a substantial digital shift within the past two decades, one might expect cassette tapes or vinyl records to be a thing of the past, yet they persevere as indicated by thousands of new DIY releases every year. This article investigates the cultural and economic significance of split releases in contemporary DIY music scenes. In contrast to music streaming or digital downloads distributed online, it argues that the technological limitations and material idiosyncrasies of cassette tapes and vinyl records used for split releases allow for creative collaboration and intentional dissociation that contextualise people throughout various cultural and geographical domains. As a result, split releases are specifically used to contrast the overwhelming possibilities that digital music production and distribution pose
DIY House Shows and Music Venues in the US. Ethnographic Explorations of Place and Community. By David Verbuč. New York, Routledge, 2022, 282 pp. ISBN: 978-1-032-04917-5
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Symbolic DIY environmentalism between sustainability statements and green pressure
No full textThis article argues that environmental sustainability statements do not constitute a one-dimensional service but encapsulate a reciprocal power relation between producers and consumers’ expectations for continuously improving sustainability standards. Drawing on Bowen's investigation of post-greenwashing assessments of corporate environmentalism, I will frame these expectations as ‘green pressure’. As there is currently no substantial research on the complex connection between Do-it-yourself (DIY) cultural production, environmentalism and green pressure, the primary concern of this article is to demonstrate the necessity for an analytical framework that grasps the symbolic characteristics of environmental practices in DIY-based alternative leisure and production circuits.Full Tex
Outsiders in Outsider Cities? Expatriates in the DIY Music Scenes of Nagoya and Fukuoka
No full textCoverage of independent music from Japan commonly focuses on the major metropolitan areas of the Kantō region, evolving around the sprawl of Tōkyō and Yokohama in the east, and the Kansai expanse formed around the cities of Ōsaka, Kyōto, Kōbe and Nara in the west of Japan’s main island of Honshū. In one sense, this focus is not surprising, as numerous influential independent music projects such as Fushitsusha, Hijokaidan, Mainliner, Hadaka no Rarı̄zu (Les Rallizes Dénudés), Boris and Merzbow emerged from the culturally and economically opposing forces of the Kansai and Kantō regions throughout the twentieth century (Novak 2013: 111ff.). Nonetheless, smaller and geographically separated cities such as Nagoya and Fukuoka host established DIY music scenes that draw on these cities’ particular local traits and culture to shape their own identity of music-making. Artists from Tōkyō and Ōsaka tour through Nagoya and Fukuoka and DIY labels release CDs, vinyl records and cassette tapes of local as well as national and international artists (see Discogs 2021; Galaxy Train 2021; Martin 2016c). Although acting outside the major urban centres, these music scenes participate in the overall production and distribution of independent music in Japan and on an international scale.No Full Tex
How does materiality ‘bite back’? Investigating cassette tapes in local, translocal and virtual music scenes
No full textDespite its status as an analogue sound carrier, the cassette has shown remarkable resilience in the digital era. Drawing on qualitative data gathered in three significant markets for cassettes, Japan, Australia and the USA, during 2018 and 2019, this article explores how the cassette tape’s material significance in the 21st century manifests itself in a complex network of interrelated local, translocal and virtual practices of music creation, distribution and consumption. The article draws on Magaudda’s ‘circuit of practice’ concept and Peterson and Bennett’s three tier model of scenes (local, trans-local and virtual). Taking the cassette’s hybrid occurrence and usage throughout a plethora of highly distinctive music scenes in Japan, Australia and the United States as a basis, we argue that Magaudda’s ‘circuit of practice’ theory needs to be structurally extended to grasp the multifaceted circuits of music in the digital age. This requires that the occurrence of a single audio format such as the cassette is recurringly analysed within different cultural contexts in order to map and delineate the format’s overall significance for contemporary music practices.No Full Tex
Ethanol Modulates The Synthesis And Catabolism Of Retinoic Acid In The Rat Prostate
No full textAll-trans retinoic acid (atRA) maintains physiological stability of the prostate, and we reported that ethanol intake increases atRA in the rat prostate; however the mechanisms underlying these changes are unknown. We evaluated the impact of a low- and high-dose ethanol intake (UChA and UChB strains) on atRA metabolism in the dorsal and lateral prostate. Aldehyde dehydrogenase (ALDH) subtype 1A3 was increased in the dorsal prostate of UChA animals while ALDH1A1 and ALDH1A2 decreased in the lateral prostate. In UChB animals, ALDH1A1, ALDH1A2, and ALDH1A3 increased in the dorsal prostate, and ALDH1A3 decreased in the lateral prostate. atRA levels increased with the low activity of CYP2E1 and decreased with high CYP26 activity in the UChB dorsal prostate. Conversely, atRA was found to decrease when the activity of total CYP was increased in the UChA lateral prostate. Ethanol modulates the synthesis and catabolism of atRA in the prostate in a concentration-dependent manner.5319Fields, A.L., Soprano, D.R., Soprano, K.J., Retinoids in biological control and cancer (2007) J Cell Biochem, 102, pp. 886-898Altucci, L., Leibowitz, M.D., Ogilvie, K.M., de Lera, A.R., Gronemeyer, H., RAR and RXR modulation in cancer and metabolic disease (2007) Nat Rev Drug Discov, 6, pp. 793-810Kane, M.A., Folias, A.E., Wang, C., Napoli, J.L., Ethanol elevates physiological all-trans retinoic acid levels in select loci through altering retinoid metabolism in multiple loci: a potential mechanism of ethanol toxicity (2010) FASEB J, 24, pp. 823-832Lasnitzki, I., Goodman, D.S., Inhibition of the effects of methylcholanthrene on mouse prostate in organ culture by vitamin A and its analogs (1974) Cancer Res, 34, pp. 1564-1571Vezina, C.M., Allgeier, S.H., Fritz, W.A., Moore, R.W., Strerath, M., Bushman, W., Retinoic acid induces prostatic bud formation (2008) Dev Dyn, 237, pp. 1321-1333Pöschl, G., Seitz, H.K., Alcohol and cancer (2004) Alcohol Alcohol, 39, pp. 155-165Alizadeh, F., Bolhassani, A., Khavari, A., Bathaie, S.Z., Naji, T., Bidgoli, S.A., Retinoids and their biological effects against cancer (2014) Int Immunopharmacol, 18, pp. 43-49Kumar, S., Sandell, L.L., Trainor, P.A., Koentgen, F., Duester, G., Alcohol and aldehyde dehydrogenases: retinoid metabolic effects in mouse knockout models (2011) Biochim Biophys Acta, 1821, pp. 198-205Molotkov, A., Duester, G., Retinol/ethanol drug interaction during acute alcohol intoxicationin mice involves inhibition of retinol metabolism to retinoic acidby alcohol dehydrogenase (2002) J Biol Chem, 277, pp. 22553-22557Lee, M.O., Manthey, C.L., Sladek, N.E., Identification of mouse liver aldehyde dehydrogenases that catalyze the oxidation of retinaldehyde to retinoic acid (1991) Biochem Pharmacol, 42, pp. 1279-1285Vasiliou, V., Pappa, A., Estey, T., Role of human aldehyde dehydrogenases in endobiotic and xenobiotic metabolism (2004) Drug Metab Rev, 36, pp. 279-299Ross, A.C., Zolfaghari, R., Cytochrome P450s in the regulation of cellular retinoic acid metabolism (2011) Annu Rev Nutr, 31, pp. 65-87Chithalen, J.V., Luu, L., Petkovich, M., Jones, G., HPLC-MS/MS analysis of the products generated from all-trans-retinoic acid using recombinant human CYP26A (2002) J Lipid Res, 43, pp. 1133-1142Taimi, M., Helvig, C., Wisniewski, J., Ramshaw, H., White, J., Amad, M., A novel human cytochrome P450, CYP26C1, involved in metabolism of 9-cis and all-trans isomers of retinoic acid (2004) J Biol Chem, 279, pp. 77-85Chung, J., Veeramachaneni, S., Liu, C., Mernitz, H., Russell, R.M., Wang, X.D., Vitamin E supplementation does not prevent ethanol-reduced hepatic retinoic acid levels in rats (2009) Nutr Res, 29, pp. 664-670Liu, C., Russell, R.M., Seitz, H.K., Wang, X.D., Ethanol enhances retinoic acid metabolism into polar metabolites in rat liver via induction of cytochrome P450 2E1 (2001) Gastroenterology, 120, pp. 179-189Lieber, C.S., De Carli, L.M., Hepatotoxicity of ethanol (1991) J Hepathol, 12, pp. 394-401Wang, X.D., Chronic alcohol intake interferes with retinoid metabolism and signaling (1999) Nutr Rev, 57, pp. 51-59Chung, J., Liu, C., Smith, D.E., Seitz, H.K., Russell, R.M., Wang, X.D., Restoration of retinoic acid concentration supressesethanolenhanced c-Jun expression and hepatocyte proliferation in rat liver (2001) Carcinogenesis, 22, pp. 1213-1219Fontanelli, B.A.F., Chuffa, L.G.A., Teixeira, G.R., Amorim, J.P.A., Mendes, L.O., Pinheiro, P.F.F., Chronic ethanol consumption alters all-trans-retinoic acid concentration and expression of their receptors on the prostate: a possible link between alcoholism and prostate damage (2013) Alcohol Clin Exp Res, 37, pp. 49-56Pasquali, D., Thaller, C., Eichele, G., Abnormal level of retinoic acid in prostate cancer tissues (1996) J Clin Endocrinol Metab, 81, pp. 2186-2191Richter, F., Joyce, A., Fromowitz, F., Wang, S., Watson, J., Watson, R., Immunohistochemical localization of the retinoic acid receptors in human prostate (2002) J Androl, 23, pp. 830-838Mardones, J., Segovia-Riquelmi, N., Thirty-two years of rats by ethanol preference: UChA and UChB strains (1983) Neuro Behav Toxicol Teratol, 5, pp. 171-178Quintanilla, M.E., Israel, Y., Sapag, A., Tampier, L., The UChA and UChB rat lines: metabolic and genetic differences influencing ethanol intake (2006) Addict Biol, 11, pp. 310-323Chuffa, L.G., Fioruci-Fontanelli, B.A., Mendes, L.O., Fávaro, W.J., Pinheiro, P.F., Martinez, M., Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment (2013) PLoS One, 8, p. e81676Chuffa, L.G., Seiva, F.R., Fávaro, W.J., Amorim, J.P., Teixeira, G.R., Mendes, L.O., Melatonin and ethanol intake exert opposite effects on circulating estradiol and progesterone and differentially regulate sex steroid receptors in the ovaries, oviducts, and uteri of adult rats (2013) Reprod Toxicol, 39, pp. 40-49Chuffa, L.G., Amorim, J.P., Teixeira, G.R., Mendes, L.O., Fioruci, B.A., Pinheiro, P.F., Long-term exogenous melatonin treatment modulates overall feed efficiency and protects ovarian tissue against injuries caused by ethanol-induced oxidative stress in adult UChB rats (2011) Alcohol Clin Exp Res, 35, pp. 1498-1508Chuffa, L.G., Seiva, F.R., Fávaro, W.J., Teixeira, G.R., Amorim, J.P., Mendes, L.O., Melatonin reduces LH, 17 beta-estradiol and induces differential regulation of sex steroid receptors in reproductive tissues during rat ovulation (2011) Reprod Biol Endocrinol, 9, p. 108Guo, X., Knudsen, B.S., Peehl, D.M., Ruiz, A., Bok, D., Rando, R.R., Retinol metabolism and lecithin: retinol acyltransferase levels are reduced in cultured human prostate cancer cells and tissue specimens (2002) Cancer Res, 62, pp. 1654-1661Kim, H., Lapointe, J., Kaygusuz, G., Ong, D., Li, C., Rijn, M.V., The retinoic acid synthesis gene ALDH1a2 is a candidate tumor suppressor in prostate cancer (2005) Cancer Res, 65, pp. 8118-8124Reichman, M.E., Hayes, R.B., Ziegler, R.G., Schatzkin, A., Taylor, P.R., Kahle, L.L., Serum vitamin A and subsequent development of prostate cancer in the first National Health and Nutrition Examination Survey Epidemiologic Follow-up Study (1990) Cancer Res, 50, pp. 2311-2315Touma, S.E., Perner, S., Rubin, M.A., Nanus, D.M., Gudas, L.J., Retinoid metabolism and ALDH1A2 (RALDH2) expression are altered in the transgenic adenocarcinoma mouse prostate model (2009) Biochem Pharmacol, 78, pp. 1127-1138Shmueli, O., Horn-Saban, S., Chalifa-Caspi, V., Shmoish, M., Ophir, R., Benjamin-Rodrig, H., GeneNote: wholegenome expression profiles in normal human tissues (2003) C R Biol, 326, pp. 1067-1072Su, A.I., Wiltshire, T., Batalov, S., Lapp, H., Ching, K.A., Block, D., A gene atlas of the mouse and human protein-encoding transcriptomes (2004) Proc Natl Acad Sci USA, 101, pp. 6062-6067McSorley, L.C., Daly, A.K., Identification of human cytochrome P450 isoforms that contribute to all-trans-retinoic acid 4-hydroxylation (2000) Biochem Pharmacol, 60, pp. 517-526Roberts, E.S., Vaz, A.D.N., Coon, M.J., Role of isozymes of rabbit microsomal cytochrome P-450 in the metabolism of retinoic acid, retinol, and retinal (1992) Mol Pharmacol, 41, pp. 427-433Abu-Abed, S., Dolle, P., Metzger, D., The retinoic acid-metabolizing enzyme, CYP26A1, is essential for normal hindbrain patterning, vertebral identity, and development of posteriorstructures (2001) Genes Dev, 15, pp. 226-24
Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
No full textBromodeoxyuridine (BrdU) is a thymidiie analogue that incorporates into DNA of dividing cells during the S-phase of the cell cycle. Previous work in laboratories reported that treatment with lOyM BrdU in the human lung carcinoma cell line (DLKP) resulted in increased expression of the cytoskeletal proteins Keratin 8 and 18 and the cell adhesion proteins a2 and b1 integrin.
This study investigated protein expression changes in differentiating DLKP cells following exposure to 10yM BrdU. DLKP cells were grown in culture flasks and harvested after 7 days exposure to BrdU. Two-dimensional gel electrophoresis was used to investigate BrdU specific changes in the proteome of DLKP BrdU treated and control cells.
Cy3-labeled DLKP control were combined with Cy-5 labeled BrdU DLKP treated proteins and separated on the same 2-D gel along with a Cy-2 labelled mixture of both samples as an internal standard. Using DIGE technology, the statistically significant comparisons of each protein abundance was made over three biological replicates. 43 protein spots were identified as differentially regulated. Among the 43 protein spots, 25 were found to be up-regulated and 18 were found to be downregulated
Geochemical dynamic in high andean Andisols, case study, Las Palmas microbasin
No full textIlustraciones, mapasThe dynamics of water in basins is regulated by its interaction with soil moisture, vegetation, temperature and precipitation among other factors. The changes in these interactions can be used as tools for the analysis of hydrological processes and generate ecosystem management proposals. The soil is a key element in the water cycle, which regulates most of the processes that take place there, but the growth of anthropogenic agro-industrial activities has brought with it the alteration of ecosystems and a significant deterioration in the quality of surface and underground waters, thus the use of nitrogenous fertilizers has been one of the factors that has led to the progressive pollution of water quality, generating a risk to human health, from the chemical species associated with these fertilizers, the contaminant that requires the most attention is nitrates.
In order to analyze the influence of pollutants such as nitrate on water quality in a basin system where andisol soils are present, the study area was divided into four areas of interest (potato cultivation, pastures, forests and the channel of Las Palmas brook), in the land-use-zones potato crops, grasslands and forests, two experimental plots with sufficient hydrometeorological instruments were used to obtain data on precipitation, surface runoff, infiltration at 0,20 and 0,50 m deep, in addition to a physicochemical analysis of the waters of each of these systems and an analysis of the dynamics of iron in Andisol soils as an indicator of ion mobility. In the interest to relate the hydrological processes based on the chemical species studied, multivariate statistical methods were applied such as the variant analysis of three factors and principal components (PCA), together with linear regressions (ANCOVA); with the purpose of generating information to design better management strategies for the different land uses and mitigate the impacts on bodies of water.
The results found show a lower storage of water in the soil for the forest soils (gallery or riparian forest) due to the high interception by the covers; while soils under crops (Solanum Tuberosum) and grazing (Pennisetum Clandestinum) have high moisture retention. Given the high availability of water in these ecosystems, conditions associated with anaerobic processes prevail in wet seasons and aerobic processes in dry seasons, where water flows in wet seasons have concentrations of less than 50 mg/L NO3-, which does not represent a risk to human health, in dry seasons these concentrations can be exceeded, especially in the forest area with concentrations up to 139 ml/L NO3- (June). The high moisture content of the soil, high rainfall, high infiltration (83-99%) and low runoff (<1%), as well as acidity and a redox potential of less than 450 mV, condition the mobility of contaminants in these environments favoring the resilience of these ecosystems.The dynamics of water in basins is regulated by its interaction with soil moisture, vegetation, temperature and precipitation among other factors. The changes in these interactions can be used as tools for the analysis of hydrological processes and generate ecosystem management proposals. The soil is a key element in the water cycle, which regulates most of the processes that take place there, but the growth of anthropogenic agro-industrial activities has brought with it the alteration of ecosystems and a significant deterioration in the quality of surface and underground waters, thus the use of nitrogenous fertilizers has been one of the factors that has led to the progressive pollution of water quality, generating a risk to human health, from the chemical species associated with these fertilizers, the contaminant that requires the most attention is nitrates. In order to analyze the influence of pollutants such as nitrate on water quality in a basin system where andisol soils are present, the study area was divided into four areas of interest (potato cultivation, pastures, forests and the channel of Las Palmas brook), in the land-use-zones potato crops, grasslands and forests, two experimental plots with sufficient hydrometeorological instruments were used to obtain data on precipitation, surface runoff, infiltration at 0,20 and 0,50 m deep, in addition to a physicochemical analysis of the waters of each of these systems and an analysis of the dynamics of iron in Andisol soils as an indicator of ion mobility. In the interest to relate the hydrological processes based on the chemical species studied, multivariate statistical methods were applied such as the variant analysis of three factors and principal components (PCA), together with linear regressions (ANCOVA); with the purpose of generating information to design better management strategies for the different land uses and mitigate the impacts on bodies of water. The results found show a lower storage of water in the soil for the forest soils (gallery or riparian forest) due to the high interception by the covers; while soils under crops (Solanum Tuberosum) and grazing (Pennisetum Clandestinum) have high moisture retention. Given the high availability of water in these ecosystems, conditions associated with anaerobic processes prevail in wet seasons and aerobic processes in dry seasons, where water flows in wet seasons have concentrations of less than 50 mg/L NO3-, which does not represent a risk to human health, in dry seasons these concentrations can be exceeded, especially in the forest area with concentrations up to 139 ml/L NO3- (June). The high moisture content of the soil, high rainfall, high infiltration (83-99%) and low runoff (<1%), as well as acidity and a redox potential of less than 450 mV, condition the mobility of contaminants in these environments favoring the resilience of these ecosystems.La dinámica del agua en las cuencas está regulada por su interacción con la humedad del suelo, la vegetación, la temperatura y la precipitación, entre otros factores. Los cambios en estas interacciones pueden ser utilizadas como herramientas para el análisis de los procesos hidrológicos y generar propuestas de gestión de los ecosistemas. El suelo es un elemento clave en el ciclo del agua, que regula la mayoría de procesos que allí se desarrollan, pero el crecimiento de actividades antropogénicas agroindustriales ha traído consigo la alteración de los ecosistemas y un deterioro marcado de la calidad de las aguas superficiales y subterráneas, así el uso de fertilizantes nitrogenados ha sido de los factores que ha llevado a la contaminación progresiva de la calidad del agua generando un riesgo para la salud humana, de las especies químicas asocias a estos fertilizantes, el contaminante que mayor atención tiene son los nitratos. Con el fin de analizar la influencia de contaminantes como el nitrato en la calidad de agua en un sistema cuenca donde están presenten los suelos andisoles, se realizó una división de la zona de estudio en cuatro áreas de interés (cultivo de papa, pastizales, bosques y el cauce de la quebrada Las Palmas), en las zonas de uso del suelo cultivos de papa, pastizales y bosques, se trabajó con dos parcelas experimentales, con los instrumentos hidrometeorológicos suficientes para la obtención de datos de precipitación, escorrentía superficial, infiltración a 0,20 y 0,50 m de profundidad, además de un análisis fisicoquímico de las aguas de cada uno de estos sistemas y un análisis de la de dinámica del hierro en suelos Andisoles como indicador de movilidad de iones. Para poder relacionar los procesos hidrológicos en función de las especies químicas estudiadas, se aplicaron métodos estadístico multivariantes, como análisis de varianza de tres factores y componentes principales (PCA), junto a regresiones lineales (ANCOVA). con el propósito de generar información para diseñar mejores estrategias del manejo de los diferentes usos del suelo y mitigar los impactos a los cuerpos de agua. Los resultados encontrados muestran un menor almacenamiento de agua en el suelo para suelos forestales (bosque de galería o ripario) debido a la alta interceptación por parte de las coberturas; mientras los suelos bajo cultivos de papa (Solanum tuberosum) y pastoreo (Pennisetum clandestinum) tienen una alta retención de humedad. Dada la alta disponibilidad de agua en estos ecosistemas prevalecen las condiciones asociadas a procesos anaeróbicos en épocas húmedas y procesos aeróbicos en épocas secas, en donde los flujos de agua en estaciones húmedas tienen concentraciones inferiores a 50 mg/L NO3- , lo cual no representa un riesgo para la salud humana, en épocas secas se pueden superar estas concentraciones especialmente en la zona de bosques con concentraciones de hasta 139 mg/L NO3- (junio). El alto contenido de humedad del suelo, las altas precipitaciones, alta infiltración (83 - 99 %) y la baja escorrentía (< 1 %), así como la acidez y un potencial redox inferior a los 450 mV, condicionan la movilidad de contaminantes en estos ambientes favoreciendo la resiliencia de estos ecosistemas. (Texto tomado de la fuente)MaestríaMaestría en Ingeniería- Recursos HidráulicosHidrogeoquímicaÁrea Curricular de Medio Ambient
