1,720,975 research outputs found
High resolution FTIR spectroscopy for new insights into oncological issues
No full textLa Microspettroscopia Infrarossa a Trasformata di Fourier (FTIRM) è un valido strumento analitico ad alta risoluzione che accoppia la spettroscopia IR con la microscopia visibile. Questo setup innovativo permette di ottenere un’analisi imaging iper-spettrale di cellule e tessuti, identificando la composizione, la struttura delle principali macromolecole e loro distribuzione topografica nei campioni analizzati.
Il mio progetto aveva lo scopo di approfondire rilevanti problematiche diagnostiche e terapeutiche riguardanti le neoplasie dei distretti testa collo (come il carcinoma orale a cellule squamose della lingua, OTSCC) e uterino (leiomioma e leiomiosarcoma). A questo scopo, sono stati usati due diversi detector IR, il single point MCT e il multipoint FPA: il primo (risoluzione spaziale ~20 m) usato per analizzare i meccanismi di interazione farmaco/cellula, il secondo (risoluzione spaziale ~2.56 m) per ottenere immagini IR di campioni di tessuto ad alta risoluzione, per migliorare la diagnosi tramite l’individuazione di marker spettrali tumore-specifici. Data la grande quantità di informazioni ottenute da un singolo campione, è necessario analizzare i dati IR tramite un approccio statistico multivariato come l’analisi gerarchica dei cluster e l’analisi delle componenti principali.
Nella mia tesi, ho combinato l’approccio spettroscopico e quello statistico per approfondire i seguenti temi: (i) investigare il ruolo del collagene nella regione peritumorale di campioni bioptici di OTSCC a diversi stadi (S1-S4); (ii) valutare i meccanismi di azione di chemioterapici (Cisplatino e 5-Azacitidina) e integratori (omega-3) su campioni cellulari di OTSCC (cellule primarie, staminali e linee), con particolare attenzione alla chemioresistenza al cisplatino; (iii) caratterizzare il collagene overespresso nelle patologie fibrotiche, come il leiomioma, e (v) identificare specifici marker spettrali per rafforzare la diagnosi del leiomioma e leiomiosarcoma uterino.Fourier Transform InfraRed Microspectroscopy (FTIRM) represents a reliable high-resolution tool which couples IR spectroscopy with visible microscopy. This innovative setup let obtain a hyper-spectral imaging analysis of tissues and cells, identifying both the composition and structure of specific macromolecules and their topographical distribution within the samples.
This PhD project aims to exploit FTIRM for deepening relevant diagnostic and therapeutic issues on neoplasms of the Head and Neck compartment (Oral Tongue Squamous Cell Carcinoma, OTSCC), and the uterine district (uterine leiomyoma, and leiomyosarcoma). To this purpose, two different IR detectors were used, the single point MCT (Hg-Cd-Te) detector and the multi point FPA (Focal Plane Array) one: the former (spatial resolution of ca. 20 m) used to investigate the mechanisms involved in the drug/cell interaction, the second (spatial resolution of 2.56 m) exploited to obtain high resolution IR images of tumor tissue samples, to improve the diagnosis by identifying tumor-specific spectral markers. The huge amount of information acquired on a single sample of tissue or cells makes it necessary submitting the IR data to a multivariate statistical approach such as the Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA).
In this PhD thesis, this spectroscopic/statistical approach was applied to: (i) investigate the role of collagen in the peritumoral region of OTSCC tissue biopsies (stages from S1 to S4); (ii) in-vitro evaluate the mechanisms of action of chemotherapy drugs (Cisplatin and 5-Azacytidine) and supplements (omega-3 fatty acids) on OTSCCs (primary, cancer stem cells and cell lines), with a particular focus on cisplatin chemoresistance effects; (iii) characterize the collagen overexpressed in fibrotic disease such as uterine leiomyoma, and (iv) identify specific spectral biomarkers to strengthen the diagnosis of uterine leiomyoma and leiomyosarcoma
The great need to overcome osimertinib resistance in advanced non-small cell lung cancer: from combination strategies to fourth-generation tyrosine kinase inhibitors
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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