500 research outputs found
Ihna Thayer Frary Home photograph
No full textThis is a photograph of the I.T. Frary Home on Bellfield Avenue in Cleveland. It measures 5.5" x 3.5" (12.7 x 8.89 cm). This photograph was taken by Ihna Thayer Frary. The Ihna Thayer Frary Audiovisual Collection was given to the Ohio Historical Society by Mr. Frary in two sections. One was in March of 1963 and the remainder in May of 1965 by his sons, Dr. Spencer G. and Allen T. Frary following their father's death. I.T. Frary (1873-1965) was the publicity and membership secretary for the Museum of Art in Cleveland, Ohio. He taught for many years at the Cleveland Institute of Art and Western Reserve University's School of Architecture. He did much research of Ohio and American architecture and was the author of seven major works and numerous scholarly articles on architectural and art history. One of his major works was Early Homes of Ohio published in 1936
German Cultural Garden photographs
No full textThree photographs document the German Cultural Garden in Cleveland, Ohio. The third view is of the Goethe-Schiller statue, dedicated to two of Germany's greatest poet-philosophers. The statue originally stood in Wade Park. The Ihna Thayer Frary Audiovisual Collection was given to the Ohio Historical Society by Mr. Frary in two sections. One was in March of 1963 and the remainder in May of 1965 by his sons, Dr. Spencer G. and Allen T. Frary following their father's death. I.T. Frary (1873-1965) was the publicity and membership secretary for the Museum of Art in Cleveland, Ohio. He taught for many years at the Cleveland Institute of Art and Western Reserve University's School of Architecture. He did much research of Ohio and American architecture and was the author of seven major works and numerous scholarly articles on architectural and art history. One of his major works was Early Homes of Ohio published in 1936
Quantitative 3-D texture analysis of interphase cell nuclei
No full textIn order to investigate the spatio-temporal structure of chromatin in interphase nuclei the authors present two 3-D texture parameters based on the grey-weighted distance transform that quantify the accessibility and the homogeneity of a nucleus. Results of experiments on computer generated textures show that these texture parameters are shape independent
Cleveland Church of the Covenant photographs
No full textThese two photographs are views of the Church of the Covenant in Cleveland, Ohio. The Church of the Covenant, a Presbyterian church, located at 11205 Euclid Ave. in University Circle, was established in 1920 as a result of mergers of three churches. The Euclid St. (United) Presbyterian Church was originally located at Euclid and E. 14th St., and was organized in 1853. Known as the Euclid Ave. Church after 1880, it merged in 1906 with Beckwith Memorial Presbyterian Church (organized in 1885), located at Fairmount (E. 107th) St. and Deering St., and became the Euclid Ave. Presbyterian Church. In 1909 this church constructed an English Gothic building on land adjacent to Women's College of Western Reserve University. Designed by Cram and Ferguson, the building featured a rose window and a 140 foot tower between the parish house and sanctuary. In 1920 the Second Presbyterian Church (chartered in 1837), located at the corner of Prospect St. and E. 30th St., joined the Euclid Ave. Presbyterian Church at its Euclid Ave. location. The name was then changed to Church of the Covenant. The photographs are 5" x 7" (12.7 x 17.78 cm). The photographs were taken by Ihna Thayer Frary. The Ihna Thayer Frary Audiovisual Collection was given to the Ohio Historical Society by Mr. Frary in two sections. One was in March of 1963 and the remainder in May of 1965 by his sons, Dr. Spencer G. and Allen T. Frary following their father's death. I.T. Frary (1873-1965) was the publicity and membership secretary for the Museum of Art in Cleveland, Ohio. He taught for many years at the Cleveland Institute of Art and Western Reserve University's School of Architecture. He did much research of Ohio and American architecture and was the author of seven major works and numerous scholarly articles on architectural and art history. One of his major works was Early Homes of Ohio published in 1936
Cleveland School of Art photograph
No full textThis is a photograph of the Cleveland School of Art taken circa 1923-1924. It measures 5.5" x 3.5" (12.7 x 8.89 cm). In 1891 the Cleveland School of Art was chartered as a private, independent art school. By 1906, the Cleveland School of Art had established a department of teacher training, and, in 1911, Western Reserve University's College for Women and the Cleveland School of Art offered their first joint degree. The Cleveland Board of Education established a cooperative program with the University in 1920 to supply teacher training. The collaborative arrangement with the School of Art (soon to be renamed the Cleveland Institute of Art) continued to mature, and, by the mid 1940s, the two institutions were collaborating on a number of educator-training degree options. By the 1970s, a joint CWRU/CIA master's degree program had been added, as had various specific teacher-certification programs. The CWRU/CIA program had become the region's premier training ground for elementary and secondary art teachers. The photographs were taken by Ihna Thayer Frary. The Ihna Thayer Frary Audiovisual Collection was given to the Ohio Historical Society by Mr. Frary in two sections. One was in March of 1963 and the remainder in May of 1965 by his sons, Dr. Spencer G. and Allen T. Frary following their father's death. I.T. Frary (1873-1965) was the publicity and membership secretary for the Museum of Art in Cleveland, Ohio. He taught for many years at the Cleveland Institute of Art and Western Reserve University's School of Architecture. He did much research of Ohio and American architecture and was the author of seven major works and numerous scholarly articles on architectural and art history. One of his major works was Early Homes of Ohio published in 1936
Ukrainian Cultural Garden photographs
No full textThese two photographs show Cleveland's Ukrainian Cultural Garden, located on the west side of the lower boulevard, opposite the Greek Garden level. The garden consitss of brick and stone courts connected by paved walks to produce a richly formal effect in a background setting of varying shades of green. The entrance is to the left court through a stone and iron gateway bearing bronze plaques and portrait reliefs by Frank L. Jirouch, representing Bohdan Khmelnitsky (1593-1657), leader of a revolt against the Poles in 1614, and Mikhail Hrushevsky (1866-1934), a historian, teacher, and author. The garden features three bronze busts of famous Ukrainians-Ivan Franko (1856-1916), poet, patriot, and folklorist Volodimir the Great (956-1015), first Christian ruler of the Ukraine and Taras G. Shevchenko (1814-1861), poet, teacher, reformer, liberator of Serfs in Russia whose popular poems have won him the name of the Father of Ukrainian Literature. These three busts are the work of Alexander Archipenko, world-famous master of modern art and one of the founders of cubism, who was born in Kiev, the ancient capital of Ukraine. The Cleveland Cultural Gardens, located along East Boulevard and Martin Luther King Jr. Boulevard, are landscaped gardens with statuary honoring Cleveland's ethnic groups. During the dedication of the Shakespeare Garden in 1916, it was decided that similar sites should be prepared for each of the city's ethnic communities. The first of these gardens, the Hebrew garden, was established in 1926. On May 9, 1927 the city set aside areas of Rockefeller Park for future gardens. The Italian, German, Lithuanian, Slovak, and Ukrainian gardens were established in 1930; the Polish, Hungarian, Czech, and Yugoslav gardens in 1934; and the American, Russian, Irish, Greek, and Syrian gardens in 1938. Romanian, Estonian, Afro-American, Chinese, Finnish, and Indian gardens have since been created. Planning and fundraising for each garden was undertaken within the ethnic communities, while the Cleveland Cultural Garden Federation (established in 1925) oversaw planning and coordinated various joint programs. The Ihna Thayer Frary Audiovisual Collection was given to the Ohio Historical Society by Mr. Frary in two sections. One was in March of 1963 and the remainder in May of 1965 by his sons, Dr. Spencer G. and Allen T. Frary following their father's death. I.T. Frary (1873-1965) was the publicity and membership secretary for the Museum of Art in Cleveland, Ohio. He taught for many years at the Cleveland Institute of Art and Western Reserve University's School of Architecture. He did much research of Ohio and American architecture and was the author of seven major works and numerous scholarly articles on architectural and art history. One of his major works was Early Homes of Ohio published in 1936
Solid-State Camera System for Fluorescence Lifetime Microscopy
No full textFluorescence microscopy is a well-established platform for biology and biomedical research (Chapter 2). Based on this platform, fluorescence lifetime imaging microscopy (FLIM) has been developed to measure fluorescence lifetimes, which are independent of fluorophore concentration and excitation intensity and offer more information about the physical and chemical environment of the fluorophore (Chapter 3). The frequency domain FLIM technique offers fast acquisition times required for dynamic processes at the sub-cellular level. A conventional frequency-domain FLIM system employs a CCD camera and an image intensifier, the gain of which is modulated at the same frequency as the light source with a controlled phase shift (time delay). At the moment these systems, based on modulated image intensifiers, have disadvantages such as high cost, low image quality (distortions, low resolution), low quantum efficiency, prone to damage by overexposure, and require high voltage sources and RF amplifiers. These disadvantages complicate the visualization of small sub-cellular organelles that could provide valuable fundamental information concerning several human diseases (Chapter 3 and 4). In order to characterize the constraints involved in current fluorescent microscope systems that are used for lifetime as well as intensity measurements and to design and fabricate new systems, we have constructed a mathematical model to analyze the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy (FLIM) (Chapter 5). The power of the light source needed for illumination in a FLIM system and the signalto-noise ratio (SNR) of the detector have led us to a photon “budget”. A light source of only a few milliWatts is sufficient for a FLIM system using fluorescein as an example. For every 100 photons emitted, around one photon will be converted to a photoelectron, leading to an estimate for the ideal SNR for one fluorescein molecule in an image as 5 (14 dB). We have performed experiments to validate the parameters and assumptions used in the mathematical model. The transmission efficiencies of the lenses, filters, and mirrors in the optical chain can be treated as constant parameters. The Beer-Lambert law is applicable to obtain the absorption factor in the mathematical model. The Poisson distribution assumption used in deducing the SNR is also valid. We have built compact FLIM systems based on new designs of CCD image sensors that can be modulated at the pixel level. Two different designs: the horizontal toggled MEM-FLIM1 camera and vertical toggled MEM-FLIM2 camera are introduced (Chapter 6). By using the camera evaluation techniques described in Chapter 7, these two versions of the MEM-FLIM systems are extensively studied and compared to the conventional image intensifier based FLIM system (Chapter 8). The low vertical charge transport efficiency limited the MEM-FLIM1 camera to perform lifetime experiments, however, the MEM-FLIM2 camera is a success. The MEM-FLIM2 camera not only gives comparable lifetime results with the reference intensifier based camera, but also shows a much better image quality and reveals more detailed structures in the biological samples. The novel MEM-FLIM systems are able to shorten the acquisition time since they allows recording of two phase images at once. The MEM-FLIM2 camera is, however, not perfect. It can only be modulated at a single frequency (25 MHz) and requires that the light source be switched off during readout due to an aluminum mask that had a smaller area than intended. A redesign of the architecture based on the vertical toggling concept leads to the MEM-FLIM3 camera (Chapter 9). Several improvements have been made in the sensor design for the MEMFLIM3 camera, such as higher fill factor, greater number of pixels etc. The MEM-FLIM3 camera is able to operate at higher frequencies (40, 60 and 80 MHz) and has an option for electron multiplication. Evaluations of this updated MEM-FLIM system are presented (Chapter 10). The images obtained from the MEM-FLIM3 camera at 20 and 40 MHz can be used directly for the lifetime calculation and the obtained lifetimes are comparable with the ones from the reference camera. There are, however, differences in the even and odd columns (20 MHz) and four image sections (40 MHz) for the intensity and lifetime images. For higher frequencies (60 and 80 MHz) calibrations are needed before calculating lifetimes. The lifetimes derived from the modulation depth after the calibrations are in a reasonable range while the lifetime derived from the phase cannot be used. At 60 and 80 MHz we can use one phase register from the MEM-FLIM3 camera for the lifetime calculation, the same way the reference camera operates. The lifetimes obtained by this method from the MEM-FLIM3 at 60 and 80 MHz are comparable with the ones from the reference camera. The MEM-FLIM3 camera also has an electron multiplication feature for low-light experimental condition. We could get approximately 500 times multiplication. Lifetime measurement using the EM function, however, has not been tested due to the limitation of the project time.Imaging Science & TechnologyApplied Science
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