2,408 research outputs found

    Staley, Roberta

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    currentAcademic Biography BA (University of Calgary) Diploma Journalism (Grant MacEwan) MA Liberal Studies (Simon Fraser University) Roberta Staley is an author, a magazine editor and writer, and a documentary filmmaker who has reported from such places as Afghanistan, Papua New Guinea, Kenya, El Salvador, Haiti, Colombia, Cambodia, South Africa, Israel, and New Zealand. She currently edits Enterprise magazine, and is a contributor to BC Business, the South China Morning Post Magazine, Ms. Magazine, Trek, the Canadian Chemical News, Corporate Knights, and Sculpture, among others. She is also a columnist for Just for Canadian Doctors/Dentists magazines. Roberta has published her first book, titled Voice of rebellion : how Mozhdah Jamalzadah brought hope to Afghanistan. It is a biography of Afghan-Canadian human rights activist Mozhdah Jamalzadah

    Saints' lives and miracle stories in Bede, the old english Bede and Ælfric between translation and rewriting

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    The aim of the present study is to explore the ways in which selected hagiographic sections of Bede’s Historia ecclesiastica gentis Anglorum have been rendered in the anonymous Old English translation of the Historia ecclesiastica, and in the Homilies and in the Lives of Saints written by Ælfric of Eynsham. The analysis is focused on five different saintly figures, each embodying a different model of sanctity in Bede’s Historia ecclesiastica: St. Alban, the martyr; Æthelthryth, virgin queen and abbess; Oswald, king of Northumbria, warrior, and saint; Fursey, a model of monastic peregrinatio who has several visions of the otherworld, and Dryhthelm, a layman who embraces monastic life after experiencing a vision of the afterlife. For every saintly figure, I develop a comparative analysis between the source text and the two target texts; each of them, in their own way, is representative of a different stage in the development of the English pre-Conquest literary system. This study combines a philologically oriented approach to the study of Medieval literature with the theoretical framework developed in the interdisciplinary field of Translation Studies. This descriptive approach allows me to address issues concerning the relationship between the ideas of translation and rewriting. It also shows that the boundary between the two ideas is far from being rigidly fixed, because perceptions of fidelity, the main parameter that defines translation as opposed to rewriting, are themselves subject to change and cannot be reduced to the mere notion of semantic equivalence. With regard to the specific texts examined here, the theoretical framework provided by Translation Studies also allows us to observe the evolution of the hagiographic genre, of its aims, and narrative strategies, within two very different contexts of production: historiography for the Historia ecclesiastica and its Old English translation, homiletics for Ælfric

    Al servizio degli altri. Lavoro di cura e lavoro per la comunità negli Enti di Terzo Settore dell’Emilia-Romagna

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    L’articolo si propone di analizzare il contributo degli Enti di Terzo Settore in Emilia-Romagna in termini di occupazione (dimensioni, qualità, tendenze). In particolare saranno illustrati e commentati i dati del “Primo censimento permanente sulle Istituzioni Non Profit” realizzato dall’Istat (2017) comparando, per quanto possibile, le risultanze relative alla nostra regione con quelle nazionali al fine di metterne in luce similarità e differenze (specificità). Il presente contributo intende approfondire l’apporto delle risorse umane (volontari e personale retribuito) con riferimento ai diversi settori di intervento in cui operano gli ETS: Cultura, sport e ricreazione; Istruzione e ricerca; Sanità; Assistenza sociale e protezione civile; Ambiente; Sviluppo economico e coesione sociale; Tutela dei diritti e attività politica; Filantropia e promozione del volontariato; Cooperazione e solidarietà internazionale; Religione; Relazioni sindacali e rappresentanza di interessi. Nonché in relazione alle diverse tipologie giuridiche ed organizzative: associazioni riconosciute; associazioni non riconosciute; cooperative sociali; fondazioni; istituzioni con altra forma giuridica (enti ecclesiastici civilmente riconosciuti, comitati, società di mutuo soccorso, istituzioni sanitarie o educative, imprese sociali con forma giuridica di impresa)

    Pigment-pigment interactions in Lhca4 antenna complex of higher plants photosystem I.

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    The red-most fluorescence emission of photosystem I (733 nm at 4 K) is associated with the Lhca4 subunit of the antenna complex. It has been proposed that this unique spectral feature originates from the low energy absorption band of an excitonic interaction involving chlorophyll A5 and a second chlorophyll a molecule, probably B5 (Morosinotto, T., Breton, J., Bassi, R., and Croce, R. (2003) J. Biol. Chem. 278, 49223-49229). Because of the short distances between chromophores in Lhc proteins, the possibility that other pigments are involved in the red-shifted spectral forms could not be ruled out. In this study, we have analyzed the pigment-pigment interactions between nearest neighboring chromophores in Lhca4. This was done by deleting individual chlorophyll binding sites by mutagenesis, and analyzing the changes in the spectroscopic properties of recombinant proteins refolded in vitro. The red-shifted (733 nm) fluorescence peak, the major target of this analysis, was lost upon mutations affecting sites A4, A5, and B5 and was modified by mutating site B6. In agreement with the shorter distance between chlorophylls A5 and B5 (7.9 Å) versus A4 and A5 (12.2 Å) in Lhca4 (Ben-Shem, A., Frolow, F., and Nelson, N. (2003) Nature 426, 630-635), we conclude that the low energy spectral form originates from an interaction involving pigments in sites A5 and B5. Mutation at site B6, although inducing a 15-nm blue-shift of the emission peak, maintains the red-shifted emission. This implies that chromophores responsible for the interaction are conserved and suggests a modification in the pigment organization. Besides the A5-B5 pair, evidence for additional pigment-pigment interactions between chlorophylls in sites B3-A3 and B6-A6 was obtained. However, these features do not affect the red-most spectral form responsible for the 733-nm fluorescence emission band

    Energy transfer among CP29 chlorophylls: calculated Förster rates and experimental transient absorption at room temperature

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    The energy transfer rates between chlorophylls in the light harvesting complex CP29 of higher plants at room temperature were calculated ab initio according to the Förster mechanism (Förster T. 1948, Ann. Physik. 2:55-67). Recently, the transition moment orientation of CP29 chlorophylls was determined by differential linear dichroism and absorption spectroscopy of wild-type versus mutant proteins in which single chromophores were missing (Simonetto R., Crimi M., Sandonà D., Croce R., Cinque G., Breton J., and Bassi R. 1999. Biochemistry. 38:12974-12983). In this way the Q(y) transition energy and chlorophyll a/b affinity of each binding site was obtained and their characteristics supported by reconstruction of steady-state linear dichroism and absorption spectra at room temperature. In this study, the spectral form of individual chlorophyll a and b ligands within the protein environment was experimentally determined, and their extinction coefficients were also used to evaluate the absolute overlap integral between donors and acceptors employing the Stepanov relation for both the emission spectrum and the Stokes shift. This information was used to calculate the time-dependent excitation redistribution among CP29 chlorophylls on solving numerically the Pauli master equation of the complex: transient absorption measurements in the (sub)picosecond time scale were simulated and compared to pump-and-probe experimental data in the Q(y) region on the native CP29 at room temperature upon selective excitation of chlorophylls b at 640 or 650 nm. The kinetic model indicates a bidirectional excitation transfer over all CP29 chlorophylls a species, which is particularly rapid between the pure sites A1-A2 and A4-A5. Chlorophylls b in mixed sites act mostly as energy donors for chlorophylls a, whereas site B5 shows high and bidirectional coupling independent of the pigment hosted

    Dynamics of chromophore binding to Lhc proteins in vivo and in vitro during operation of the xanthophyll cycle.

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    Three plant xanthophylls are components of the xanthophyll cycle in which, upon exposure of leaves to high light, the enzyme violaxanthin de-epoxidase (VDE) transforms violaxanthin into zeaxanthin via the intermediate antheraxanthin. Previous work () showed that xanthophylls are bound to Lhc proteins and that substitution of violaxanthin with zeaxanthin induces conformational changes and fluorescence quenching by thermal dissipation. We have analyzed the efficiency of different Lhc proteins to exchange violaxanthin with zeaxanthin both in vivo and in vitro. Light stress of Zea mays leaves activates VDE, and the newly formed zeaxanthin is found primarily in CP26 and CP24, whereas other Lhc proteins show a lower exchange capacity. The de-epoxidation system has been reconstituted in vitro by using recombinant Lhc proteins, recombinant VDE, and monogalactosyl diacylglycerol (MGDG) to determine the intrinsic capacity for violaxanthin-to-zeaxanthin exchange of individual Lhc gene products. Again, CP26 was the most efficient in xanthophyll exchange. Biochemical and spectroscopic analysis of individual Lhc proteins after de-epoxidation in vitro showed that xanthophyll exchange occurs at the L2-binding site. Xanthophyll exchange depends on low pH, implying that access to the binding site is controlled by a conformational change via lumenal pH. These findings suggest that the xanthophyll cycle participates in a signal transduction system acting in the modulation of light harvesting versus thermal dissipation in the antenna system of higher plants
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