1,261 research outputs found
In vitro reconstitution of the recombinant photosystem II light harvesting complex CP24 and its spectroscopic characterization
No full textThe light-harvesting chlorophyll a/b protein CP24, a minor subunit of the photosystem II antenna system, is a major violaxanthin-binding protein involved in the regulation of excited state concentration of chlorophyll a. This subunit is poorly characterized due to the difficulty in isolation and instability during purification procedures. We have used an alternative approach in order to gain information on the properties of this protein; the Lhcb6 cDNA has been overexpressed in bacteria in order to obtain the CP24 apoprotein, which was then reconstituted in vitro with xanthophylls, chlorophyll a, and chlorophyll b, yielding a pigment-protein complex with properties essentially identical to the native protein extracted from maize thylakoids. Although all carotenoids were supplied during refolding, the recombinant holoprotein exhibited high selectivity in xanthophyll binding by coordinating violaxanthin and lutein but not neoxanthin or beta-carotene. Each monomer bound a total of 10 chlorophyll a plus chlorophyll b and two xanthophyll molecules
Novel aspects on pigment binding protein CP24 and of the recombinant protein reconstituted “in vitro"
No full textRicostituzione in vitro del complesso del fotosistema II CP24 e sua caratterizzazione spettroscopica
No full text
Space and the nation: three texts on Aldo Rossi
No full textIn this paper the spatial dimensions of political practice and the historical dimensions of architectural practice are examined. The author argues that these two practices intersect when, in the life of a city and a nation, time is transformed into space. The productivity of death in this regard is explored. In developing this argument, reference is made to the works and writings of Regis Debray and Aldo Rossi, as well as events in the recent political history of South Africa
Foul Air: Aldo van Eyck on Postmodern Architecture
No full textIntroduction to bookGreen Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Space & Typ
Pulmonary involvement in adult Still's disease: Case report and brief review of literature
No full textBackground: Adult onset Still disease (AOSD) is a rare condition characterized by elevated fever along with arthritic symptoms, elevated polymorphonuclear neutrophil count, evanescent rash, and hyperferritinemia. Diagnosis can be made only after have ruled out more frequent conditions, and Yamagouchi or Fautrel criteria should be applied. Parenchimal lung involvement (PLI) is present in less than 5% of AOSD cases and ranges from aspecific reticular interstitial opacities to life threatening conditions, such as acute respiratory distress syndrome (ARDS).
Case: We report the case of a 59 years old man who was referred to our ward because of high fever treated as a pneumonia with antibiotic but not responding to medical treatment, and findings suggestive of interstitial lung disease prevalent in the lower zone on high resolution computed tomography (HRCT). AOSD was diagnosed when the diagnostic Yamaguchi criteria were fulfilled and our suspect was confirmed by the rheumatologist; the patient was then successfully treated with corticosteroids.
Conclusions: PLI in AOSD is very rare but must be considered among differential diagnosis in patients with high fever and aspecific interstial parenchimal lung involvement
NOVEL BIOMARKERS FOR DIAGNOSIS AND/OR PROGNOSIS OF NEOPLASIAS IN ANIMALS
No full textThe invention is generally applicable in the field of biomedical engineering, and specifically relates to a method for diagnosis and/or prognosis of neoplasias in animals. The invention further relates to a diagnostic/prognostic kit associated to such method, a reagent for the preparation of such kit and the use of particular biomarkers in such method and/or kit. The method comprises at leas the steps of : drawing at least one sample from the patient and determining the amount of a biomarker in said at least one sample drawn from the patient. The biomarker is a protein released by pancreatic cells
Higher plants light harvesting proteins. Structure and functions as revealed by mutational analysis of either protein or chromophore moietes.
No full textMutation analysis of higher plants light harvesting proteins has been prevented for a long time by the lack of a suitable expression system providing chromophores essential for the folding of these membrane-intrinsic pigment-protein complexes. Early work on in vitro reconstitution of the major light harvesting complex of photosystem II (LHCII) indicated an alternative way to mutation analysis of these proteins. A new procedure for in vitro refolding of the four light harvesting complexes of photosystem II, namely CP24, CP29, CP26 and LHCII yields recombinant pigment-proteins indistinguishable from the native proteins isolated from leaves. This method allows both the performing of single point mutations on protein sequence and the exchange of the chromophores bound to the protein scaffold. We review here recent results obtained by this method on the pigment-binding properties, on the chlorophyll-binding residues, on the identification of proton-binding sites and on the role of xanthophylls in the regulation of light harvesting function
- …
