1,721,013 research outputs found
Effects of 25-OH vitamin D supplementation on mesenchymal stromal cells in an animal model of Systemic Lupus Erythematosus (SLE)
No full textIn systemic lupus erythematous (SLE), mesenchymal stromal cells (MSCs) are defective and may present early signs of senescence. In a previous study we demonstrated as the treatment with hydroxychloroquine may influence the clonogenic potential of MSCs in a SLE animal model.
Vitamin D may influence several aspects of the innate and adaptative immune response and low serum levels of vitamin D are common in SLE patients and were correlated to higher disease activity and severity. The aim of this study is to identify the effect of 25-OH vitamin D, on MSCs characteristics in New Zealand Black/White F1 mice (NZ), that develop a disease very similar to human SLE.
We are treating 20 NZ with a diet enriched with 25-OH-vitamin-D 10.000 UI/Kg, mice starting from 8 weeks of age. We divided the mice in 4 experimental groups (5 mice each), the first group will be sacrificed before the start of the treatment (8 week of age) and the other groups will be sacrificed at 16, 26 and 36 weeks of age. Fifteen mice were enrolled as controls and they will be sacrificed at 16, 26 and 36 weeks of age.
We are evaluating disease activity parameters including total urinary protein parameters, anti-dsDNA autoantibody, 25OH vitamin D ant parathormone levels.
MSC phenotype is confirmed by cytofluorimetry (expression of Sca-1 and CD-106, negativity for CD45) and we also evaluate differentiation capability in adipogenic and osteoblast lineage. Clonogenic potential is evaluated in at the seeding (P0) and in the following 2 passages (P1 and P2 respectively one and two weeks after the seeding) with the Colony Forming Unit (CFU) assay
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
One year in review 2017: idiopathic inflammatory myopathies
No full textEvery year new concepts about pathogenesis, serology, diagnosis and treatment in inflammatory myopathies (IIMs) have been provided. The purpose of this manuscript is to summarise the most relevant literature contributions published over the last year about these complex and rare diseases
Management of digital ulcers in systemic sclerosis
Full text linkSystemic sclerosis (SSc) is a rare disease characterized by autoimmune pathogenesis, alterations to the vascular system, and fibrosis of the skin and internal organs. Digital ulcers (DUs) are common in SSc patients, and represent a major burden for the patients. The management of DUs in systemic sclerosis is difficult, because it needs a multimodal therapeutic approach, as local treatment alone is usually insufficient and also systemic treatment with vasoactive drugs and modification of the lifestyle are usually required for healing of the wounds. In this review, we describe the optimal management of DUs according to recent literature and our clinical practice for systemic and local treatment of chronic digital wounds in SSc patients
One year in review 2016: idiopathic inflammatory myopathies
No full textIdiopathic inflammatory myopathies (IIM) are a group of rare, acquired, clinically heterogeneous autoimmune inflammatory muscle disorders characterised by muscle weakness and multisystem involvement. Recently, new concepts about pathogenesis, diagnosis and treatment of these complex diseases have been provided. The purpose of this manuscript is to summarise the most relevant literature contributions published over the last year
One year in review 2015: idiopathic inflammatory myopathies
No full textIdiopathic inflammatory myopathies (IIM) are a group of rare acquired muscle diseases that mainly affect skeletal muscles. Recently, novel insights into the pathogenesis, diagnosis and treatment of these complex diseases have been provided. Herewith we provided an overview of the most significant literature contributions published over the year
Idiopathic inflammatory myopathies: one year in review 2022
No full text: Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of disorders in which chronic inflammation of the skeletal muscle, leading to muscle weakness, is a common feature. Different phenotypes have been identified within the IIM spectrum based on extra-muscular manifestations, immunology, muscle histology, responsiveness to therapy, and prognosis. The pathogenesis, classification, treatment, and prognosis of the different IIM subtypes are subject to active discussion and research. This review highlights the most relevant literature published on this topic over the last year
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