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    FIG. 4. — Lepthyphantes ensiferus Barrientos n in The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco

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    FIG. 4. — Lepthyphantes ensiferus Barrientos n. sp., genital organs: A, B, male copulatory bulb, retrolateral view (A), ventral view (B); C, schema of the embolic division; D, E, epigyne, ventral view (D), lateral view (E). Abbreviations: see Material and methods. Scale bar: 0.5 mm.Published as part of Barrientos, José Antonio, Brañas, Neus & Mederos, Jorge, 2020, The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco, pp. 1-29 in Zoosystema 42 (1) on page 8, DOI: 10.5252/zoosystema2020v42a1, http://zenodo.org/record/362705

    FIG. 11. — Palliduphantes banderolatus Barrientos n in The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco

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    FIG. 11. — Palliduphantes banderolatus Barrientos n. sp., genital organs: A, B, male copulatory bulb, retrolateral view (A), ventral view (B); C, schema of the embolic division; D-F, epigyne, ventral view (D), lateral view (E), posterior view (F). Abbreviations: see Material and methods. Scale bar: 0.5 mm.Published as part of Barrientos, José Antonio, Brañas, Neus & Mederos, Jorge, 2020, The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco, pp. 1-29 in Zoosystema 42 (1) on page 19, DOI: 10.5252/zoosystema2020v42a1, http://zenodo.org/record/362705

    A note on the Bandwidth choice when the null hypothesis is semiparametric

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    This work presents a tool for the additivity test. The additive model is widely used for parametric and semiparametric modeling of economic data. The additivity hypothesis is of interest because it is easy to interpret and produces reasonably fast convergence rates for non-parametric estimators. Another advantage of additive models is that they allow attacking the problem of the curse of dimensionality that arises in non- parametric estimation. Hypothesis testing is based in the well-known bootstrap residual process. In nonparametric testing literature, the dominant idea is that bandwidth utilized to produce bootstrap sample should be bigger that bandwidth for estimating model under null hypothesis. However, there is no hint so far about how to choose such bandwidth in practice. We will discuss a first step to find some rule of thumb to choose bandwidth in that context. Our suggestions are accompanied by simulation studies.additive models, bootstrap, bootstrap test, kernel smoothing, nonparametric

    Lepthyphantes biospeleologorum Barrientos 2020, n. sp.

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    Lepthyphantes biospeleologorum Barrientos n. sp. (Figs 3; 13C, D) urn:lsid:zoobank.org:act: 87BA13BB-23F2-41C3-B5AA-1C7B1A5C6992 TYPE MATERIAL. — Holotype. Morocco. 1♀; Ifri Bouyzem, Aglefth, Taglefth, Azilal; 15.V.2004; F. Fadrique leg.; MZB 2017-0660. Paratypes. Morocco. 2♀; same locality and date as the holotype; MZB 2017-0661. OTHER MATERIAL. — Morocco. 4 juv.; same locality and date as the holotype; MZB 2017-0662. DIAGNOSIS. — The epigyne is broad and small, protruding on the ventral side of the epigastrum. The bursa copulatrix has a very short front edge; the lateral edges expand and separate to accommodate the scape; the posterior wall differentiates a median plate, narrow in the center and rounded on the sides. The proscape is wide and flattened, with rounded contour and a notch in the anterior part; a short and rounded strecher in the distal part of the scape, but flanked by two rounded expansions. By the morphology of its epigyne (and without information on the male copulatory bulb) this species has some affinities with L. brevihamatus, L. longihamatus and, in general, with the entire “ afer group”, in which probably it should be placed. ETYMOLOGY. — This species is dedicated, in a general way, to all the biospeleologists. Anonymously they use part of their time to develop an exciting and hard (sometimes risky) activity, in pursuit of the forms that inhabit the caverns. Without their generous dedication, this small job would not have been possible. DISTRIBUTION. — This species is exclusively known from the type locality. DESCRIPTION Female (holotype) Body. Total body length 2.30. Carapace: 0.93 long by 0.83 wide; yellowish brown color, and with a tenuous pigmentary pattern formed by five radial gray bands and one marginal in thoracic area. Cephalic part with some scattered hairs. Eyes developed and well pigmented; arranged in two transversal lines, with individual black areolas in each eye. Chelicerae: 0,39 long; coloration slightly more intense than the carapace; with three equidistant teeth on the promargin, the basal one somewhat smaller; retromargin unarmed. Legs long and thin; brown color, like the caparace; coloration gradually lost from the tibia towards apex. Measurements: leg I (1.47 + 0.27 + 1.57 + 1.35 + 0.86), leg II (1.30 + 0.29 + 1.22 + 1.27 + 0.83), leg III (1.00 + 0.27 + 0.98 + 0.91 + 0.64), and leg IV (1.37 + 0.29 + 1.20 + 1.27 + 0.93). Opisthosoma 1.42 long by 1.00 wide; gray-white color, without dorsal pattern, but entirely covered with a fine hairiness. Female genitalia. Epigyne (Figs 3; 13C, D) with a transverse development, protruding little in ventral side of epigastrum. Its basal part showing a wide bursa copulatrix, with a very short anterior wall; lateral walls expanding and separate to accommodate the scape; posterior wall wide and reinforced by a sclerotized median plate, narrowed in the center and rounded on the sides. Anterior and lateral walls with a thick, sparse and well-arched hairiness. Scape starting from the anterior part of the bursa, and with a wide and flat proscape, rounded on the sides and with a notch on its distal part (connecting with the middle part); fertilization ducts visible in submarginal position. Median part of scape inflected and hidden under the proscape; the distal part, tourning towards it again, dilating and showing below the proscape; strecher short and rounded, but flanked by two rounded expansions. Distal part usually curved outwards, so that its distal part approaches the proscape. Male Unknown.Published as part of Barrientos, José Antonio, Brañas, Neus & Mederos, Jorge, 2020, The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco, pp. 1-29 in Zoosystema 42 (1) on page 6, DOI: 10.5252/zoosystema2020v42a1, http://zenodo.org/record/362705

    Additional genetic requirements for RFA1-MN cell viability in the absence and presence of calcium

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    figure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado (A–D) Effect of the indicated mutations in the growth of RFA1-MN cells as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells onto SMM medium without or with the indicated concentrations of CaCl2. The analyses were repeated at least twice with similar results. Mutants scored in the SGA screening are shown in bold.Peer reviewe

    Melanoplus parvus Barrientos-Lozano & Rocha-Sanchez, n. sp.

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    Melanoplus parvus Barrientos-Lozano & Rocha-Sánchez n. sp. (Figs. 27 a–f– 28 a–c; 30 a–e; 31 a–c, 32 a) Type material (material examined).— Holotype 3 and allotype Ƥ. Mexico, Coahuila, El Cascajal, Los Lirios, Arteaga, 1942 m, Lat. 25 ° 22.591 ’’N, Long. 100 ° 46.17 ’’W, 26.IX. 2009, Barrientos-Lozano L., Méndez-Gómez B. R. & Rocha-Sánchez A. Y. Paratypes, Coahuila.— 2 3, 1 Ƥ, same data as holotype, coll. L Barrientos-Lozano-ITCV; 1 Ƥ, Jamé, road (rd.) Nuncio-Rayones, 2394 m, Lat. 25 ° 21.33 ”N, Long. 100 ° 35.588 ”W, 27.IX. 2009, Barrientos-Lozano L., Méndez-Gómez B. R. & Rocha-Sánchez A. Y, coll. L. Barrientos-Lozano-ITCV. Diagnosis.— M. par vus n. sp., may be distinguished from congeneric species as follows: very unique elongated and stylized cerci (Figs 27 c, d; 28 b), as long as the supra-anal plate, broad basally, incurved and narrowing about mid portion, then widening, distally the upper margin bent conspicuously upwards and inwards; the upper margin rounded laterally and, in dorsal view, the ventral acute. The supra-anal plate triangular, proximally broad, furculae obsolete, side margins almost straight, medio-longitudinal sulcus broad proximally, moderately deep, about ¾ the length of the supra-anal plate; the subgenital plate subconical, distinctly tuberculate apically (Figs. 27 d; 28 c). M. par vus n. sp., presents certain similarities with M. strumosus Morse, i.e., in general appearance, size and cerci (Fig. 29 a–c). However, we are not comparing M. par vus n. sp. with M. strumosus because the latter species occurs only in southeastern United States, as all other members of the Puer Group, to which M. strumosus is assigned. Furthermore, characters that define the Puer Group are not clearly established. Description of male.— A small species, average 15 mm in length. General body color brown, head medium size, face whitish-cream, antennae brownish, eyes relatively large for its size and prominent, interocular space rather wide, fastigium of vertex gently declevent, enlarging apically. Pronotum (Fig. 27 a, b), light brown dorsally, moderately widening posteriorly, anterior margin emarginated, posterior margin rounded and emarginated, metazona densely punctate, median carina prominent-dark brown, postocular band dark brown-blackish, diverging gently posteriorly and fading on metazona, mesoepimeron and anterior portion of metapleuron dark brownblackish, a white stripe along metaepimeron, lower portion of lateral lobes whitish-cream with this color extending on to lower portion of mesopleuron, tegmina short, ovate, attingent, brownish-half darker on lower portion, veins cream. Pro, meso and metathoracic femora tumescent, brownish, hind tibiae bluish. Abdomen with a dark brown band on each side-fading on the last three abdominal tergites, subgenital plate (Figs. 27 c, d; 28 c) subconical with a conspicuous tubercle apically. Supra-anal plate triangular, proximally broad, side margins almost straight, mediolongitudinal sulcus broad basally, moderately deep, beyond mid length, furculae obsolete (Figs. 27 d; 28 a). Cerci (Figs. 27 c, d; 28 b) elongated, as long as the supra-anal plate, broad basally, incurved and narrowing about mid portion, then widening, distally the upper margin bent conspicuously upwards and inwards; the upper margin rounded laterally and, in dorsal view, the ventral acute. Epiphallus (Figs. 27 e; 30 d, e) with medium size ancorae, shorter than the anterior process; anterior process moderately developed, distally pointed and projecting inwards; lophi large and prominent; broad bridge; posterior processes distally produced. Phallic complex as shown in Figs. 27 f; 30 a–c. Aedeagal valves (Fig. 31 a–c): Dorsal valvae tubular, distally broader and concave; ventral valvae elongated, basally broad, tapering gradually, half distal curved inwards, apex moderately expanded and rounded. Description of female.— Similar to the males, average 17 mm in length, general body color brown. The head medium size, face and genae whitish cream, eyes medium size and moderately prominent, interocular space wider than in males, antennae brownish. Pronotum dorsally brown, weakly widening posteriorly, metazone punctate, median carina prominent-dark brown, posterior margin gently emarginated, postocular band dark brown fading on metazone, lower portion of pronotal lateral lobes whitish cream, mesopleuron dark brown, metaepimeron with a white stripe. Tegmina short, ovate, attingent, brownish, lower portion darker. Pro, meso and metathoracic femora light brown, hind femora darker along the upper half of the outer face, with black apices; hind tibiae bluish. Cerci and ovipositor’ valves as shown in Fig. 32 a. Measurements (mm). Males.— Body length from vertex to end of hind femur: 15.0 (14.0–15.0). Pronotum length: 3.4 (3.1–3.8). Tegmina length: 2.8 (2.7–2.9). Hind femora length: 7.8 (7.8–7.9). Females: Body length: 17.0 (16.0–17.0). Pronotum length: 3.7 (3.5–3.9). Tegmina length: 3.2 (3.1–3.2). Hind femora length: 9.3 (9.2– 9.4). Distribution.— Species collected in Coahuila, Mexico, Sierra de Arteaga, at two localities: Jamé, 2394 m and El Cascajal, 1942 m (Fig. 32 b). Habitat.— The Sierra de Arteaga in the state of Coahuila, is part of the mountain ranges that form the northern portion of the Easter Sierra Madre (ESM), located north of the Tropic of Cancer in the temperate latitudinal zone (Fig. 33). The forest here is in a transition zone between the semiarid High Plateau and cool-temperate mountains of the ESM. Elevation ranges from 1,900 to 3,400 m. The climate in the area is sub-humid temperate. The average annual temperature and rainfall are 17 °C and 498 mm, respectively; rainfall is convective and matches with the warm months of the year (July-October). Lithosols are predominant and represent 49 % of the area, while Rendzina prevails in the foothills and valleys and represent 29 %. Vegetation types are represented by montane shrub, oak forest (Quercus), pine forest (pinyon pine forest.— P. cembroides), high altitude conifer forest (Abies and Pseudotsuga) and quaking aspen forest (Populus). M parvus n. sp., lives on herbaceous plants such as Piqueria trinervia, Anthemis sp., Senecio sp., (Asteraceae); Salvia sp., Mentha sp., Origanum sp., (Lamiaceae); Muhlenbergia sp., Buchloe dactyloides (Poaceae). Etymology.— The specific name parvus, alludes to the small size of this species.Published as part of Barrientos-Lozano, Ludivina, Rocha-Sánchez, Aurora Y. & Horta-Vega, Jorge V., 2013, Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico, pp. 261-286 in Zootaxa 3669 (3) on pages 278-283, DOI: 10.11646/zootaxa.3669.3.4, http://zenodo.org/record/24756

    Por D. Ioseph Maldonado de Saavedra, en el pleyto con doña Isabel, y doña Antonia Maldonado, hijas de D. Diego Bernardino Maldonado

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    Texto firmado por "Doct. D. Roberto Ramirez de Barrientos". Texto fechado en "Cadiz, y Iunio 10 de 1663". Esc. xil. de la Compañía de Jesús en la port. -- Letra capital ornada.A FD/0101(20

    Palliduphantes megascapus Barrientos 2020, n. sp.

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    Palliduphantes megascapus Barrientos n. sp. (Figs 12; 15 D-F) urn:lsid:zoobank.org:act: 885DDD66-4851-4A1D-BC5E-812B5D20ADBE TYPE MATERIAL. — Holotype. Morocco. 1♀; Puits Cochrisco C.A. 8, Azour ou Aghroud, Tarhazoute, Agadir Ida Ou Tanane; 27.IX.2016; C. Fontgivell leg.; MZB 2016-4101. Paratypes. Morocco. 3♀; same locality and date as the holotype; MZB 2017-0668. OTHER MATERIAL. — Morocco. 1♀; Ifri Laghar (= Ifri Tagaderth, = Al Andalus), Tizgui N’Chorfa, Agadir Ida Ou Tanane; 23.VII.2003; J. Esquius leg.; MZB 2003-1261 • 2♀; same locality; 23.VII.2003; F. Fadrique & J. Esquius leg.; MZB 2003-1288 • 1♀; same locality; 13.X.2015; F. Fadrique leg.; MZB 2015-8618 • 1♀; Puits Cochrisco C.A. 10, Azour ou Aghroud, Tarhazoute, Agadir Ida Ou Tanane; 26.VII.2003; F. Fadrique & J. Esquius leg.; MZB 2013-2950 • 1♀, 1 juv.; Cv. Imi Ougoug, Assif N’Talma, Immouzzer, Agadir Ida Ou Tanane; 27.VII.2003; F. Fadrique leg.; MZB 2013-2949. DIAGNOSIS. — The epigyne is very developed, occupying a large part of the middle ventral zone of opisthosoma. Its anterior and lateral walls are lengthened and oriented backwards. The scape acquires a great development. The proscape is narrow, elongating backwards, the middle part is very long, almost reaching the opisthosomal wall, where it curves in “C” and connects with the distal part. The whole structure is very sclerotized. Its development and appearance is visible particularly laterally. ETYMOLOGY. — The name of the new species refers to the enormous development of the female genitalia, in relation to the size of the opisthosoma, especially; to the length of the scape. DISTRIBUTION. — The four cavities in which this species is found are in the province of Agadir Ida Ou Tanane (two in Tarhazoute [C.A.8 and C.A.10], one in Tizgui N’Chorfa [Ifri Laghar] and another in Immouzzer [Cv. Imi Ougoug]), in Antiatlas, southern Morocco. DESCRIPTION Female (holotype) Body. Total body length: 2.40. Carapace: 1.03 long by 0.83 wide, light brown, without additional pigmentation pattern, but with some hairs scattered on the cephalic part. Eyes developed and pigmented, with black areolas that meet the eyes MA, LA and LP; arranged in the two typical transverse lines. Chelicerae: 0.42 in length and with a similar coloration to that of carapace; three teeth on the promargin (the basal much smaller and separated from the others) and unarmed retromargin. Brown legs, with darker femurs, long and thin; measurements: leg I (1.37 + 0.27 + without + without + without), leg II (1.30 + 0.27 + 1.22 + 1.17 + 1.59), leg III (1.15 + 0.22 + 0.93 + 0.95 + 0.22), and leg IV (1.40 + 0.25 + 1.30 + 1.22 + 0.73). Opisthosoma, 1.37 long by 1.08 wide, more or less uniform grayish, and without dorsal pigmentation pattern, but covered with thin and scattered hairs. Female genitalia. Epigyne (Figs 11A, B; 15 D-F) highly developed and protruding on the ventral side of the epigastrum, tilted backwards and covering the central half of the postepigastrum. Its basal part elongate in front and on the sides, orienting itself towards the back; bursa copulatrix opening in its distal part. Anterior and lateral walls with a scattered and clearly distinct pilosity, with some long hairs areolated at their base. Posterior wall with a middle plate, not very visible because arranged against the opisthosomal surface. The side walls not forming “wings”, although they cover the base of the scape. Proscape quite narrow and not very individualized; merging with the end of the anterior wall and elongate backward, giving a rather voluminous structure. Middle part of the scape bending and facing forward. Entire scape narrowed, elongated and well sclerotized. Near the back wall, distal part of the scape widening and curved again, describing a “C. The whole structure forming a rigid and narrow structure revealing the fertilization ducts by transparency. The two receptacles also observed, at the base of the structure. Male Unknown.Published as part of Barrientos, José Antonio, Brañas, Neus & Mederos, Jorge, 2020, The high complexity of Micronetinae Hull, 1920 (Araneae, Linyphiidae) evidenced through ten new cave-dweller species from the Morocco, pp. 1-29 in Zoosystema 42 (1) on pages 20-21, DOI: 10.5252/zoosystema2020v42a1, http://zenodo.org/record/362705
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