44 research outputs found

    mouse-recapture-v1.0.0

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    <p>R code for implementing Cormack-Jolly-Seber capture recapture model for mouse enclosure experiment described in Barrett et al. Science 2018</p&gt

    The interplay between epigenomic and transcriptomic variation during ecotype divergence in stickleback

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    Background: Populations colonizing contrasting environments are likely to undergo adaptive divergence and evolve ecotypes with locally adapted phenotypes. While diverse molecular mechanisms underlying ecotype divergence have been identified, less is known about their interplay and degree of divergence. Results: Here we integrated epigenomic and transcriptomic data to explore the interactions among gene expression, alternative splicing, DNA methylation, and microRNA expression to gauge the extent to which patterns of divergence at the four molecular levels are aligned in a case of postglacial divergence between marine and freshwater ecotypes of nine-spined sticklebacks (Pungitius pungitius). Despite significant genome-wide associations between epigenomic and transcriptomic variation, we found largely non-parallel patterns of ecotype divergence across epigenomic and transcriptomic levels, with predominantly nonoverlapping (ranging from 43.40 to 87.98%) sets of differentially expressed, spliced and methylated genes, and candidate genes targeted by differentially expressed miRNA between the ecotypes. Furthermore, we found significant variation in the extent of ecotype divergence across different molecular mechanisms, with differential methylation and differential splicing showing the highest and lowest extent of divergence between ecotypes, respectively. Finally, we found a significant enrichment of genes associated with ecotype divergence in differential methylation. Conclusions: Our results suggest a nuanced relationship between epigenomic and transcriptomic processes, with alignment at the genome-wide level masking relatively independent effects of different molecular mechanisms on ecotype divergence at the gene level.</p

    Data from: Assessing reproductive isolation using a contact zone between parapatric lake-stream stickleback ecotypes

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    Ecological speciation occurs when populations evolve reproductive isolation as a result of divergent natural selection. This isolation can be influenced by many potential reproductive barriers, including selection against hybrids, selection against migrants, and assortative mating. How and when these barriers act and interact in nature is understood for relatively few empirical systems. We used a mark-recapture experiment in a contact zone between lake and stream three-spined sticklebacks (Gasterosteus aculeatus, Linnaeus) to evaluate the occurrence of hybrids (allowing inferences about reproductive isolation), the inter-annual survival of hybrids (allowing inferences about selection against hybrids), and the shift in lake-like versus stream-like characteristics (allowing inferences about selection against migrants). Genetic and morphological data suggest the occurrence of hybrids and no selection against hybrids in general, a result contradictory to a number of other studies of sticklebacks. However, we did find selection against more lake-like individuals, suggesting a barrier to gene flow from the lake into the stream. Combined with previous work on this system, our results suggest that multiple (most weakly and often asymmetric) barriers must be combining to yield substantial restrictions on gene flow. This work provides evidence of a reproductive barrier in lake-stream sticklebacks and highlights the value of assessing multiple reproductive barriers in natural contexts

    IJSAP Volume 04, Number 01

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    TABLE OF CONTENTS – VOL. 4(1), 1983 LETTERS EDITORIALS The LD50—The Beginning of the End – A.N. Rowan Some Rights for Animal Therapists: Better Science and Better Welfare – D.H. Murphy Occlusion of Vision in Old English Sheepdogs – M.W. Fox NEWS AND ANALYSIS Preliminary Verdict for Electro-Immobilization Enforcement Powers for Humane Societies? In a Natural Environment, Pig Behavior Resembles That of Wild Boars Egg Producers Issue Guidelines for Destroying Baby Chicks Establishing Baselines for Domestic Animal Behavior A Farmer’s Response to the “Downer” Cow Dilemma Attitudes Toward Dogs and Cats Deaths in Primate Trade Researching Research Methods Cat Population Dynamics Dart Gun Modifications Alternatives at NIH Laboratory Animal Numbers COMMENTS Vivisection and Misanthropy – G.P. Cave Thoughtful Use of Animals – H. Kitchen ORIGINAL AND REVIEW ARTICLES Abundance and Distribution of Large Mammals in the Upper Ogun Game Reserve, Oyo State, Nigeria – T.A. Afolayan, K.R.N. Milligan, and S.O. Salami Feral Dogs of the Galapagos Islands: Impact and Control – B.D. Barnett and R.L. Rudd The Effects of Ethostasis on Farm Animal Behavior: A Theoretical Overview – A.F. Fraser and M.W. Fox LEGISLATION AND REGULATION CURRENT EVENTS BOOK NEWS VOLUME 3 INDEX INSTRUCTIONS TO AUTHOR

    IJSAP Volume 04, Number 01

    No full text
    TABLE OF CONTENTS – VOL. 4(1), 1983 LETTERS EDITORIALS The LD50—The Beginning of the End – A.N. Rowan Some Rights for Animal Therapists: Better Science and Better Welfare – D.H. Murphy Occlusion of Vision in Old English Sheepdogs – M.W. Fox NEWS AND ANALYSIS Preliminary Verdict for Electro-Immobilization Enforcement Powers for Humane Societies? In a Natural Environment, Pig Behavior Resembles That of Wild Boars Egg Producers Issue Guidelines for Destroying Baby Chicks Establishing Baselines for Domestic Animal Behavior A Farmer’s Response to the “Downer” Cow Dilemma Attitudes Toward Dogs and Cats Deaths in Primate Trade Researching Research Methods Cat Population Dynamics Dart Gun Modifications Alternatives at NIH Laboratory Animal Numbers COMMENTS Vivisection and Misanthropy – G.P. Cave Thoughtful Use of Animals – H. Kitchen ORIGINAL AND REVIEW ARTICLES Abundance and Distribution of Large Mammals in the Upper Ogun Game Reserve, Oyo State, Nigeria – T.A. Afolayan, K.R.N. Milligan, and S.O. Salami Feral Dogs of the Galapagos Islands: Impact and Control – B.D. Barnett and R.L. Rudd The Effects of Ethostasis on Farm Animal Behavior: A Theoretical Overview – A.F. Fraser and M.W. Fox LEGISLATION AND REGULATION CURRENT EVENTS BOOK NEWS VOLUME 3 INDEX INSTRUCTIONS TO AUTHOR
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