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Temperature-induced denaturation of ribonuclease S: a thermodynamic study
No full textIn this paper the thermal denaturation of ribonuclease S, the product of mild digestion of
ribonuclease A by subtilisin, is deeply investigated by means of DSC and CD measurements. It results
that at whatever pH in the range 4-7.5 the process is fully reversible but not well represented by the
simple two-state N T D transition. Actually, a two-state model that considers both unfolding and
dissociation, NL T D + L*, well accounts for the main features of the process: the tail present in the
low-temperature side of DSC peaks and the marked dependence of denaturation temperature on protein
concentration. This mechanism is strictly linked to the exact stoichiometry of RNase S. An excess of
the protein component of RNase S, the so-called S-protein, shifts the system toward a more complex
behavior, that deserves a separate treatment in the accompanying paper [Graziano, G., Catanzano, F.,
Giancola, C., & Barone, G. (1996) Biochemistry 35, 13386-13392]. The thermodynamic analysis leads
to the conclusion that the difference in thermal stability between RNase S and RNase A is due to entropic
effects, i.e., a greater conformational flexibility of both backbone and side chains in RNase S. The process
becomes irreversible at pH 8.0-8.5, probably due to side-reactions occurring at high temperature. Finally,
the influence of phosphate ion on the stability of RNase A and RNase S at pH 7.0 is studied and explained
in terms of its binding on the active site of ribonucleases. The analysis enables us to obtain an estimate
of the apparent association constant and binding enthalpy also
Webcam and smartphone for the measure of spatial-temporal parameters of gait for treadmill use
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Lecture Notes in Electrical Engineering
Volume 392, 2016, Pages 261-272
International Workshop on Mobile Networks for Biometric Data Analysis, 2014; Ancona; Italy; 30 October 2014 through 31 October 2014; Code 179299
Webcam and smartphone for the measure of spatial-temporal parameters of gait for treadmill use (Conference Paper)
Barone, V. , Verdini, F., Di Nardo, F., Maranesi, E., Burattini, L., Fioretti, S.
Università Politecnica delle Marche, Ancona, Italy
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Abstract
A very low cost prototype has been developed for the spatial and temporal analysis of human movement using an integrated system of last generation smartphones and a high-definition webcam, controlled by a laptop. The system can be used to analyse mainly planar motions in non-structured environments. In this paper, the accelerometer signal captured by the 3D sensor embedded in one smartphone, and the position of coloured markers extracted from the analysis of the webcam frames, are used for the computation of spatial-temporal parameters of gait. The system has been tested on a treadmill at different gait speeds. Accuracy of results is compared with that obtainable by a gold-standard stereometric instrumentation. The system is characterised by a very low cost and a very high level of automation. It has been thought to be used by non-expert users in ambulatory settings
Untethering an unusual cause of kidney injury in a teenager with Down syndrome
No full textAcute kidney injury (AKI) is characterized by the acute nature and the inability of kidneys to maintain fluid homeostasis as well as adequate electrolyte and acid-base balance, resulting in an accumulation of nitrogenous waste and elevation of serum blood urea nitrogen and creatinine values. Acute kidney injury may be a single isolated event, yet oftentimes, it results from an acute chronic kidney disease. It is critical to seek out the etiology of AKI and to promptly manage the underlying chronic kidney disease to prevent comorbidities and mortality that may ensue. We described a case of a 16-year-old adolescent girl with Down syndrome who presented with AKI and electrolyte aberrance.Abdominal and renal ultrasounds demonstrated a significantly dilated bladder as well as frank hydronephrosis and hydroureter bilaterally. Foley catheter was successful in relieving the obstruction and improving her renal function. However, a magnetic resonance imaging was pursued in light of her chronic constipation and back pain, and it revealed a structural defect (tethered cord) that underlies a chronic process that was highly likely contributory to her AKI. She was managed accordingly with a guarded result and required long-term and close monitoring.Peer reviewed
Short-term effects of mitigation measures for the containment of the COVID-19 outbreak: An experience from Northern Italy
No full textWe evaluated the short-term effects of mitigation measures imposed by the Italian government on the first 10 municipalities affected by Sars-Cov-2 spread. Our results suggest that the effects of containment measures can be appreciated in about two weeks
Design by Evolution: Advances in evolutionary design
No full textPhilip F. Hingston, Luigi C. Barone, Zbigniew Michalewicz (eds.) ; foreword by David B. Fogelhttp://trove.nla.gov.au/work/3488030
Dynamic behaviors of the charge carriers investigated by means of noise spectroscopy
No full textFluctuation Spectroscopy is a very informative method to study the dynamic behavior of the charge carriers and the kinetic processes in condensed matter. Investigation of noise properties is a powerful technique, especially when complemented with standard transport measurements based on average quantities. Various materials have been tested, ranging from Mn oxides, to novel superconductors, to carbon nanotube composites. In all cases, the 1/f noise dependence on external parameters, such as temperature, bias current, and/or electric field, is usually characterized by non-standard properties
Christiano-Albertinae Inauguratio / descripta ab Alexandro Iulio Torquato. Barone et Colonello
No full textCHRISTIANO-ALBERTINAE INAUGURATIO / DESCRIPTA AB ALEXANDRO IULIO TORQUATO. BARONE ET COLONELLO
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sj-do-27-smr-10.1177_00491241221134522 - Supplemental material for Measuring Class Hierarchies in Postindustrial Societies: A Criterion and Construct Validation of EGP and ESEC Across 31 Countries
No full textSupplemental material, sj-do-27-smr-10.1177_00491241221134522 for Measuring Class Hierarchies in Postindustrial Societies: A Criterion and Construct Validation of EGP and ESEC Across 31 Countries by O. Smallenbroek, F. Hertel and C. Barone in Sociological Methods & Research</p
Future direzioni di ricerca e di applicazioni cliniche per gli strumenti di autovalutazione dell'attaccamento adulto
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