1,721,016 research outputs found
Supplementary Materials. Identification and characterization of resistance to rust in lentil and its wild relatives
No full textTable S1: Response of the Lens germplasm accessions to U. viciae-fabae ex L. culinaris studied. DS = Disease Severity (%); IT = Infection Type according Stakman et al. (1962) [29], 6 where IT 0 = no symptoms, IT ; = necrotic flecks, IT 1 = minute pustules barely sporulating, IT 2 = necrotic halo surrounding small pustules, IT 3 = chlorotic halo and IT 4 = well-formed pustules with 7 no associated chlorosis or necrosis. 8 Ns = not studiedThis research was funded by the Spanish Agencia Estatal de Investigación (AEI) grant PID2020-114668RB-100.Peer reviewe
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives
No full textLentil rust is a major disease worldwide caused by Uromyces viciae-fabae. In this study, we screened a large germplasm collection of cultivated lentils (Lens culinaris ssp. culinaris) and its wild relatives, both in adult plants in the field with a local rust isolate during 2 seasons and in seedlings under controlled conditions with four fungal isolates of worldwide origin. The main results from our study were the following: (1) a significant number of accessions with resistance based on hypersensitive reaction (reduced Infection Type (IT)) were identified in cultivated lentil and in L. ervoides, L. nigricans and L.c. orientalis. The IT scores showed a clear isolate-specific response suggesting race-specificity, so each fungal isolate might be considered a different race. Resistance was identified against all isolates what might be the basis to develop a standard differential set that should be a priority for rust definition and monitoring. (2) Interestingly, although at lower frequency than in L. ervoides and L. nigricans, the hypersensitive response was also observed within cultivated lentil, with accession 1561 (L.c. culinaris) displaying resistance to the four isolates making this accession a valuable ready-to-use resource for lentil resistance breeding. Resistance to all other rust isolates was also available within L.c. culinaris in an isolate-specific manner. Accession 1308 (L. ervoides) showed resistance against all isolates tested, as well as a reduced number of accessions belonging to other wild Lens species. (3) In addition, our screenings allowed the identification of several accessions with partial resistance (reduced Disease Severity (DS) despite high IT). Adult Plant Resistance resulting in reduced severity in adult plants in the field, despite high susceptibility in seedlings, was more frequently identified in L.c. culinaris, but also in L. nigricans and L.c. orientalis
Supplementary material. Uncovering Phytotoxic Compounds Produced by Colletotrichum spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach
No full textFigure S1. m/z distribution diagrams and Base Peak Chromatograms of C. truncatum isolate C428 (from lentil) extracts. (a) PDA
extract; (b) PDB extract; (c) Rice extract; (d) Richard extract.
Figure S2. m/z distribution diagrams and Base Peak Chromatograms of C. trucatum isolate C431 (from soybean) extracts. (a) PDA
extract; (b) PDB extract; (c) Rice extract; (d) Richard extract.
Figure S3. m/z distribution diagrams and Base Peak Chromatograms of C. trifolii isolate C436 (from clover extracts. (a) PDA extract;
(b) PDB extract; (c) Rice extract; (d) Richard extract.
Figure S4. Structure of: validated metabolites with pure standards (level A; red); putatively identified and produced by Colletotrichum
spp. (level B(i), blue); putatively identified and produced by other fungal species (level B(ii), black); Structures of Curvupallide
A and B (Level C(i), brown).
Table S1. Parameters Metaboanalyst 5.0 for LC-MS spectra processing.
Table S2. Features lists transformation and scaling for PLS – DA analysis. Q2 and R2 values of PLS – DA models in cross validation.
Table S3. Metabolites dereplicated by targeted and untargeted metabolomics analysis, organised according to identification level.
Table S4. Metabolites dereplicated by targeted and untargeted metabolomics analysis, organised according to identification level.Peer reviewe
Phenotyping data of Vicia sativa germplasm collection 2021-2023
No full textCharacterization data of a Vicia sativa germplams colection grown at Córdoba, Spain under three crop seasons.PROYECTO P20_00986 PAIDI2020 Junta Andalucía.Peer reviewe
Supplementary Materials: Comparative analysis of secondary metabolites produced by Ascochyta fabae under in vitro conditions and their phytotoxicity on the primary host, Vicia faba, and related legume crops
No full textSupplementary Figure S1. 1H-NMR spectrum of ascochlorin (1) (CDCl3, 400 MHz); Supplementary Figure S2. ESI/MS (+) spectrum of ascochlorin (1); Supplementary Figure S3. 1H-NMR spectrum of ascofuranol (2) (CDCl3, 400 MHz); Supplementary Figure S4. ESI/MS (+) spectrum of ascofuranol (2); Supplementary Figure S5. 1H-NMR spectrum of (R)-mevalonolactone (3) (CDCl3, 400 MHz); Supplementary Figure S6. ESI/MS (+) spectrum of (R)-mevalonolactone (3); Supplementary Figure S7. 1H-NMR spectrum of ascosalipyrone (4) (CDCl3, 400 MHz); Supplementary Figure S8. ESI/MS (+) spectrum of ascosalipyrone (4); Supplementary Figure S9. 1H-NMR spectrum of benzoic acid (5) (CDCl3, 400 MHz); Supplementary Figure S10. ESI/MS (+) spectrum of benzoic acid (5); Supplementary Figure S11. 1H-NMR spectrum of tyrosol (6) (CDCl3, 400 MHz); Supplementary Figure S12. ESI/MS (+) spectrum of tyrosol (6); Supplementary Figure S13. 1H-NMR spectrum of ascosalitoxin (7) (CDCl3, 400 MHz); Supplementary Figure S14. ESI/MS (+) spectrum of ascosalitoxin (7); Supplementary Figure S15. Images of the symptoms of each of the compounds. Supplementary Table S1. Diseased area (mm2) measured on detached leaves of several legume crops with exudates from the fungus A. fabae growth in vitro on 3 different culture media (Czapek-Dox = CD, potato dextrose broth = PDB, and rice) at concentrations of 0.5, 1, and 2 mg/mL. Negative (blank untreated, water and MeOH 5%) controls were also included. The experiment was repeated four times. Supplementary Table S2. Diseased area (mm2) measured in leaves detached from various legume crops with metabolites produced by the exudate of the A. fabae fungus from the three-growth media at concentrations of 1, 10, and 100 µM. Negative controls (untreated blank, water, and MeOH 5%) were also included. p-value compared with value from MeOH 5% control.Peer reviewe
Dataset for "Genetic diversity and population structure of a wide Pisum spp. core collection"
No full textSilico-DArT and SNP datasets of the IAS pea core collection.
Each file contain key information of the molecular markers used to establish the population structure and genetic diversity of the IAs pea core collection.These datasets are part of the research project AGL2017-82907-R funded by MCIN/AEI/ 10.13039/501100011033 and by "ERDF A way of making Europe" and by the project PID2020-114668RB-100 funded by MCIN/AEI/ 10.13039/501100011033.Peer reviewe
Inhibition of early development stages of rust fungi by the two fungal metabolites cyclopaldic acid and epi-epoformin
No full textRusts are a noxious group of plant diseases affecting major economically important crops. Crop protection is largely based on chemical control. There is a renewed interest in the discovery of natural products as alternatives to synthetic fungicides for control. In this study we tested two fungal metabolites, namely cyclopaldic acid and epi-epoformin, for their effectiveness in reducing early stages of development of two major rust fungi from the genera Puccinia and Uromyces, P. triticina and U. pisi. Spore germination and appressorium formation were assessed on pretreated detached leaves under controlled conditions. Cyclopaldic acid and epi-epoformin were also tested in infected plants in order to evaluate the level of control achieved by treatments both before and after inoculation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
- …
