1,721,084 research outputs found
A Methodology for Co-simulation-Based Optimization of Biofabrication Protocols
Full text linkBiofabrication processes are complex and often unsatisfactory. Trial-and-error methods are costly and yield only incremental innovation, starting from sub-optimal and poorly represented existing processes. Although computational techniques might support efficient process design to find optimal process configurations, intelligent computational approaches must comprise biological complexity to provide meaningful insights. This paper proposes a novel co-simulation-based optimization methodology for the systematic design of protocols for cell culture and biofabrication. The proposed strategy integrates evolutionary computation and simulation for efficient design space exploration and assessment of candidate protocols. A generic library supports the modular and flexible composition of multiscale and multidomain co-simulation scenarios. The feasibility of the presented approach was demonstrated in the automatic generation of rotocols for the biofabrication of an epithelial cell monolayer. The results are twofold. First, the prototype co-simulation library helps build flexible, loosely coupled simulation scenarios. Second, the in-silico experimentation on the use case shows that the proposed approach is a viable first step towards standard and automated design in biofabrication
Surgical ligation of cisterna chyli: an alternative treatment for chronic chylothorax in children.Ann Thorac Surg. 2010 Nov;90(5):1732-4
No full textAnn Thorac Surg. 2010 Nov;90(5):1732-4.
Surgical ligation of cisterna chyli: an alternative treatment for chronic chylothorax in children.
Zanin A, Padalino MA, Cerutti A, Vida VL, Milanesi O, Stellin G, Bardini R.
Source
Department of Pediatrics, University of Padua-Medical School, Padova, Italy.
Abstract
Postoperative chylothorax represents one of the early and most serious complications after cardiothoracic operations. When conservative measures are not effective, an operation is mandatory. We describe a case of persistent chylothorax after a "one-and-a-half ventricle" repair in an infant with complex congenital heart disease, which was successfully treated with a transabdominal ligation of the cisterna chyli. At the 1-year follow-up, she is in excellent clinical condition, without recurrence of pleural effusion or the requirement for nutritional support. Ligation of cisterna chyli may represent a safe and effective ultimate treatment in children with persistent chylothorax.
Copyright © 2010 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.
PMID:
20971313
[PubMed - indexed for MEDLINE
Confronto dell’efficacia di due vaccini commerciali anti PCV2 in condizioni normali di allevamento - Comparative efficacy of two commercial PCV2 vaccines in conventionally pigs
No full textL’efficacia di due vaccini anti Porcine Circovirus 2 (PCV2) è stata valutata, in suini convenzionali, in termini di risposta anticorpale, quantità di genoma virale presente nel sangue, sintomi clinici e performances di accrescimento dallo svezzamento fino a 220 giorni di vita. Allo scopo è stato realizzato uno studio di campo randomizzato cieco in una azienda a ciclo chiuso di 420 scrofe, utilizzando 1050 suinetti provenienti da 5 bande consecutive di produzione, distribuiti in 3 gruppi. All’inizio della sperimentazione (25±2 giorni di vita), 349 animali (gruppo A) sono stati vaccinati intramuscolo (i.m.) con una dose di 0,5 ml
di Circovac®, 351 animali (gruppo B) hanno ricevuto una dose i.m. di 1 ml di CircoFLEX®. I 350 soggetti del gruppo C sono stati utilizzati come animali di controllo non vaccinati. Tutti i suini sono stati pesati a 25, 105 e 220 giorni di vita ed è stato calcolato l’incremento ponderale medio giornaliero (IPMG). Le valutazioni sierologiche e virologiche (quantità di genoma virale presente nel sangue, determinata mediante real time PCR), sono state condotte esaminando i campioni di sangue prelevati mensilmente (dallo svezzamento fino a 6 mesi di vita), da un gruppo di 36 soggetti selezionati in modo casuale e appartenenti
alla terza banda. Gli animali vaccinati hanno presentato un IPMG significativamente più elevato rispetto ai soggetti del gruppo di controllo nei periodi 25-105, 105-220 e 25-220 giorni di vita, minori percentuali di morti, scarti e soggetti sottopeso e minori quantità di genoma virale nel sangue. Tra i due vaccini, non è stata osservata nessuna differenza statisticamente significativa relativamente ai parametri considerati
LE ENDOPROTESI ESOFAGEE E LA LASERTERAPIA ENDOSCOPICA NEL TRATTAMENTO PALLIATIVO DEL CARCINOMA DELL'ESOFAGO E DEL CARDIAS
No full textMeta-analysis of Gene Activity (MAGA) Contributions and Correlation with Gene Expression, Through GAGAM
Full text linkIt is well-known how sequencing technologies propelled cel- lular biology research in the latest years, giving an incredible insight into the basic mechanisms of cells. Single-cell RNA sequencing is at the front in this field, with Single-cell ATAC sequencing supporting it and becoming more popular. In this regard, multi-modal technologies play a crucial role, allowing the possibility to perform the mentioned sequenc- ing modalities simultaneously on the same cells. Yet, there still needs to be a clear and dedicated way to analyze this multi-modal data. One of the current methods is to calculate the Gene Activity Matrix, which summarizes the accessibility of the genes at the genomic level, to have a more direct link with the transcriptomic data. However, this concept is not well-defined, and it is unclear how various accessible regions impact the expression of the genes. Therefore, this work presents a meta-analysis of the Gene Activity matrix based on the Genomic-Annotated Gene Ac- tivity Matrix model, aiming to investigate the different influences of its contributions on the activity and their correlation with the expression. This allows having a better grasp on how the different functional regions of the genome affect not only the activity but also the expression of the genes
How a modified Nissen procedure works: a mechanistic study using intraoperative esophageal high-resolution manometry
No full textObjective: We aimed at demonstrating how a modified Nissen procedure works by analyzing intraoperatively the variations of the low esophageal sphincter pressure values using high resolution manometry. Methods: This study included 15 patients with documented gastroesophageal reflux disease who underwent a laparoscopic modified Nissen procedure. Data regarding the changes in the pressure values were recorded at each step of the procedures using high resolution manometry and after the progressive insufflation of air in the stomach. Categorical data were compared between the preoperative and postoperative periods using Fisher’s test, and continuous data were compared using Mann–Whitney U non-parametric test. Preoperative versus postoperative variations in continuous data were assessed using Wilcoxon’s non-parametric test for paired data. Results: Intraoperative manometric data showed a rise of basal LES pressure until the creation of the wrap. An evident increase of pressure values was recorded after gastric air insufflation, as consequence of the increase of intragastric pressure. No intraoperative and postoperative complications were observed. All patients experienced a significant reduction in terms of intensity and frequency of gastroesophageal reflux symptoms and no patients complained of dysphagia. Conclusions: Intraoperative high resolution manometry was feasible in all patients and demonstrated that the modified Nissen procedure works by increasing the LES pressure in response to gastric distension, without impeding the progression of the bolus into the stomach
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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