24 research outputs found
Replication Data for: Stage-dependent remodeling of large-scale architecture of projections to primary motor cortex in ALS mouse model revealed by a new variant retrograde-AAV9
No full textRaw images displayed in the related manuscript submitted to eLif
Replication Data for: Stage-dependent remodeling of large-scale architecture of projections to primary motor cortex in ALS mouse model revealed by a new variant retrograde-AAV9
No full textRaw images displayed in the related manuscript submitted to eLif
The role of cytokinin receptors in Arabidopsis thaliana seed development and how they affect the metabolomic profile
No full textBased on expression, functional, and metabolomic analyses in the seeds of the single-receptor mutants, each receptor has a specific function during seed development. Their redundant roles during this process are difficult to assess; moreover, the impact they have on plant development must also be taken into account. In this study, we investigated the role of cytokinin receptors in Arabidopsis thaliana seed development and their impact on the metabolomic profile. Our findings reveal distinct expression patterns among them in the seed: AHK2 expression is not detected in seed tissues, AHK3 is expressed in embryo, endosperm, and peripheral endosperm, while AHK4/CRE1 expression is restricted to a few embryo cells. These patterns are consistent with the observed phenotypes where ahk3 exhibits more severe seed phenotypes such as delayed embryo development and increased seed and endosperm size. Metabolomic analyses showed that the receptors impact the abundance of metabolites, with a remarkably high concentration of tannins in ahk2 with respect to wild type seeds, while ahk3 mutant seeds have a very low amount of tannins but elevated levels of other compounds such as sinapoylated glucosinolates (GSLs), important for plant defense. The metabolic profile performed further supports a link between cytokinin and the regulation of secondary metabolites such as flavonoids and glucosinolates. Our results suggest that each cytokinin receptor independently contributes to this regulation, reflected in the distinct metabolic profiles of each mutant
Impact of phenylpropanoid compounds on heat stress tolerance in carrot cell cultures
No full textThe phenylpropanoid and flavonoid families include thousands of specialized metabolites that influence a wide range of processes in plants, including seed dispersal, auxin transport, photoprotection, mechanical support and protection against insect herbivory. Such metabolites play a key role in the protection of plants against abiotic stress, in many cases through their well-known ability to inhibit the formation of reactive oxygen species (ROS). However, the precise role of specific phenylpropanoid and flavonoid molecules is unclear. We therefore investigated the role of specific anthocyanins (ACs) and other phenylpropanoids that accumulate in carrot cells cultivated in vitro, focusing on their supposed ability to protect cells from heat stress. First we characterized the effects of heat stress to identify quantifiable morphological traits as markers of heat stress susceptibility. We then fed the cultures with precursors to induce the targeted accumulation of specific compounds, and compared the impact of heat stress in these cultures and unfed controls. Data modeling based on Projection to Latent Structures (PLS) regression revealed that metabolites containing coumaric or caffeic acid, including ACs, correlate with less heat damage. Further experiments suggested that one of the cellular targets damaged by heat stress and protected by these metabolites is the actin microfilament cytoskeleton
Large-scale bioprospection of the Italian flora for specialized metabolism mapping and biodiversity exploitation
No full textApple fruit superficial scald resistance mediated by ethylene inhibition is associated with diverse metabolic processes
No full textFruits stored at low temperature can exhibit different types of chilling injury. In apple, one of the most serious
physiological disorders is superficial scald, which is characterized by discoloration and brown necrotic
patches on the fruit exocarp. Although this phenomenon is widely ascribed to the oxidation of a-farnesene,
its physiology is not yet fully understood. To elucidate the mechanism of superficial scald development and
possible means of prevention, we performed an integrated metabolite screen, including an analysis of volatiles,
phenols and lipids, together with a large-scale transcriptome study. We also determined that prevention
of superficial scald, through the use of an ethylene action inhibitor, is associated with the triggering of
cold acclimation-related processes. Specifically, the inhibition of ethylene perception stimulated the production
of antioxidant compounds to scavenge reactive oxygen species, the synthesis of fatty acids to stabilize
plastid and vacuole membranes against cold temperature, and the accumulation of the sorbitol, which can
act as a cryoprotectant. The pattern of sorbitol accumulation was consistent with the expression profile of a
sorbitol 6-phosphate dehydrogenase, MdS6PDH, the overexpression of which in transgenic Arabidopsis
thaliana plants confirmed its involvement in the cold acclimation and freezing toleranc
Phytochemical characterization and bioprospection of Asterid clade in the Italian territory
No full textAsterid clade is the largest subgroup of eudicots, with approximately 100.000 species grouped into 17
orders representing almost 25% of all angiosperm species [1]. In the frame of Italian flora, Asterids represent
around 40% of all land plants and amounts to 45% of Italian angiosperms [2]. Plant species belonging to
Asterid clade are known for production and accumulation of different bioactive metabolites. A
significant example is represented by the antimalarial drug artemisinin, a sesquiterpene lactone isolated
from A. annua leaves [3]. The aim of this work is the bioprospection and chemical characterization of plant
metabolites inside the Asterid clade within the Italian flora, with a focus on the identification of new natural
sources for high value secondary metabolites. Additionally, data obtained from metabolomic analysis will
enable us to conduct a comparative investigation of the phytochemodiversity of Asterids specialized
metabolome. In order to cover the chemical diversity of the Italian flora inside this group, all the 47 plant
families of Asterid clade were included in the sampling plan, for a total of 221 species. The number of
selected species per family was chosen proportionally, according to the dimension of each family. Plants
were sampled from nurseries, botanical gardens and open field. So far, 206 species out 221 were sampled
during the sampling campaign, resulting in a coverage of 85% for the families and 90% the genera of the
Asterid clade included in the project. The UPLC-HR-MS analysis is currently on going, with 75 species
already analyzed
