11 research outputs found
ROOT ANALOGUE IMPLANTS- THE NEW ROUTE
No full textDental implants are an excellent option for replacing missing teeth. Traditional implants have a cylindrical or tapered geometry with threads along the screw length. The lack of proper congruency between the implant and the socket bone can eventually lead to implant failure due to stability loss and failure in osseointegration. To overcome this problem, novel approaches are being evaluated to manufacture customized root implants. This is expected to reduce the bone and soft-tissue trauma and promote a better primary stability. They do not require bone drilling, sinus lifting, bone augmentation or other traumatic procedures
Effect of Freeze Drying on the Stability of Probiotic Lactic Acid Bacteria
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Preparation and Characterization of Biodegradable Modified Chitosan Films for Food Packaging
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Role of p75 neurotrophin receptor in the development of neuronal and oligodendroglial progenitors of the rat postnatal subventricular zone
No full textThe p75 neurotrophin receptor is widely expressed in the brain during embryonic development (Frade & Barde, 1998). In neonatal and adult ages its expression in the central nervous system gets restricted to specific cell populations including cholinergic neurons of the basal forebrain, olfactory ensheathing glia, progenitors of the hippocampus and progenitors of the cerebellum where it plays a multitude of roles. While some studies have also reported that the subventricular zone (SVZ) expresses p75NTR in postnatal and adult stages (Galvão, RP, Garcea-Verdugo, JM, Alvarez-Buylla, 2008; Giuliani et al., 2004; van Strien et al., 2014; Young, Merson, et al., 2007), its exact role in this germinal niche remains unknown. Further, the expression of p75NTR has been reported at various ages in the rat and human SVZ but it is not observed in mice (Galvão, RP, Garcea-Verdugo, JM, Alvarez-Buylla, 2008). Although species differences remain unexplained, no comprehensive studies of p75NTR, regarding its role in the rat or human SVZ, have been conducted thus far. The SVZ serves as a neurogenic niche for the olfactory bulb in adult animals (Alvarez-Buylla et al., 2002; Bath & Lee, 2010; Faigle & Song, 2013). For a brief period postnatally, it also generates glial cells, predominantly oligodendrocytes for the corpus callosum, cortex and striatum (Kessaris et al., 2006.; Kuhn et al., 2019; Luskin & McDermott, 1994; Menn et al., 2006; Naruse et al., 2017). Initial characterization showed expression of p75NTR in the dorsolateral SVZ throughout postnatal development in rats with maximal expression observed during the period of gliogenesis. Therefore, I hypothesized that p75NTR may be involved in the regulation of SVZ progenitor development during postnatal development.
This study shows that p75NTR regulates both neuronal and oligodendroglial progenitors in the postnatal SVZ. Progenitors expressing p75NTR proliferate longer than cells in the dSVZ that lack p75NTR. I also determine that in the absence of p75NTR, postnatal oligodendrocyte progenitor development was accelerated in comparison to that observed under normal conditions. Consequently, I observed premature maturation of oligodendrocytes at postnatal ages which could have implications for myelination.
Furthermore, I describe a role for p75NTR in neurogenesis where p75NTR defines a specific neuronal subpopulation expressing the transcription factor Pax6. I show that, postnatally, p75NTR is expressed by proliferating cells present in the rostral migratory stream extending from the lateral ventricle to the olfactory bulb. In younger animals, absence of p75NTR did not alter the cytoarchitecture of the olfactory bulb and did not affect odor discrimination. However, lack of p75NTR functionally affected odor discrimination in aged rats and led to changes in the olfactory bulb circuitry.
Overall, this study defines a novel role for the multifunctional receptor p75NTR in the SVZ in regulation of oligodendrocyte progenitors in vivo during postnatal development. Further, I provide evidence that the role of p75NTR is not limited to a single subset of oligodendrocyte lineage committed progenitors but could have additional roles in olfactory bulb neurogenesis as a result of ageing.Ph.D.Includes bibliographical reference
Author Correction: Highly dampened blood transcriptome response in HIV patients after respiratory infection
No full textCorrection to "Highly dampened blood transcriptome response in HIV patients after respiratory infection
Author Correction: Hidden genomic features of an invasive malaria vector, Anopheles stephensi, revealed by a chromosome-level genome assembly
No full textThe original article [1] contained an error in Fig. 3 and omitted a Funding source which the authors would like to correct. Due to a labeling error in one of our Iso-Seq samples, the RNA sample that was collected 24h after blood feeding was labeled as 324 by the sequencing center because they unknowingly removed a separator between the replicate number and the sample name. The error resulted in a different ordering of categories than we would have chosen, though this doesn’t actually affect the interpretations we made in the manuscript. The corrected Fig. 3 can be viewed ahead in this correction article. Although the error does not affect any conclusion, it remains technically inaccurate and merits correction. We also did not acknowledge funding from the United States National Science Foundation (NSF) to J.J.E. for development of the sex chromosome inference approach. This is an important oversight, as NSF requires acknowledgment. The grant number is: NSF grant IOS-1656260
Data extracted from articles for meta-analysis.
No full textData included author details, year of publication, marker, Pubmed ID and case number both in Non-Dysplastic Oral Lesions (ND-OL) and Dysplastic Oral Potentially Malignant Disorders (D-OPMD). (DOCX)</p
Equity, diversity, and inclusion at the Global Alliance for Genomics and Health
No full textA lack of diversity in genomics for health continues to hinder equitable leadership and access to precision medicine approaches for underrepresented populations. To avoid perpetuating biases within the genomics workforce and genomic data collection practices, equity, diversity, and inclusion (EDI) must be addressed. This paper documents the journey taken by the Global Alliance for Genomics and Health (a genomics-based standard-setting and policy-framing organization) to create a more equitable, diverse, and inclusive environment for its standards and members. Initial steps include the creation of two groups: the Equity, Diversity, and Inclusion Advisory Group and the Regulatory and Ethics Diversity Group. Following a framework that we call "Reflected in our Teams, Reflected in our Standards," both groups address EDI at different stages in their policy development process. [Abstract copyright: © 2023 The Author(s).
Avaliação da atividade antitumoral do extrato bruto e supercrítico de Cordia verbenacea
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica, Florianópolis, 2010O câncer está entre as causas mais freqüentes de morte no mundo. É considerado um importante problema de saúde pública em países desenvolvidos e em desenvolvimento, sendo a segunda causa de morte no Brasil e no mundo, superado somente pelas doenças do sistema cardiovascular. Apesar disto até o momento não existe uma terapia efetiva para o tratamento de todos os tipos de câncer além de que a maioria dos quimioterápicos em uso apresentam elevada toxicidade. Esforços vêm sendo dirigidos no sentido de desenvolver fármacos antitumorais tão ou mais eficazes do que os quimioterápicos já disponíveis, porém com menor toxicidade e potencial para desenvolver resistência terapêutica. Muitos dos medicamentos utilizados atualmente resultaram da purificação de produtos naturais, principalmente vegetais. Neste contexto, o presente projeto objetivou avaliar o potencial antitumoral de Cordia verbenacea planta medicinal brasileira, vulgarmente conhecida como erva baleeira que é popularmente utilizada em Santa Catarina para tratamento de tumores e inflamações. Para se atingir tal objetivo foram realizados experimentos para avaliar a atividade citotóxica e antiproliferativa in vitro e antitumoral in vivo. Para tanto, realizou-se os ensaios de viabilidade celular (MTT) em células de tumor ascítico de Ehrlich (TAE) e MCF-7, proliferação celular (incorporação de [3H] timidina) e capacidade pró-poptótica (Brometo de etídio/Laranja de acridina (BE/LA)) em células TAE. Além disso, verificou-se o possível efeito dos extratos sobre o DNA plasmidial (atividade nucleásica) assim como a capacidade protetora do extrato sobre o DNA (com a geração de espécies reativas de oxigênio com Fe-EDTA). Foi avaliada a expressão da COX-2 através de Wertern blot em células MCF-7. A determinação da atividade antitumoral in vivo foi realizada em camundongos Balb/c inoculados com o TAE e tratados com extrato bruto (EB) e supercrítico (ESC) nas concentrações de 37,5; 75 e 150 mg/Kg. Nos ensaios do MTT e incorporação de timidina triciada os resultados demonstraram que o EB e ESC reduziram de maneira significativa a viabilidade e proliferação celular. A coloração com BE/LA revelou que o provável tipo de morte celular induzida pelos tratamentos trata-se de apoptose, uma vez que a grande maioria das células adquiriram uma coloração laranja-avermelhada, característica de células apoptóticas. Os extratos mostraram-se ineficazes no teste de ativação nucleásica. O ESC foi capaz de reduzir significativamente a expressão da COX-2 em células MCF-7. Os ensaios in vivo demonstram que tanto EB quanto ESC apresentaram efeitos antitumorais, sendo os melhores resultados observados para a dose de 150 mg/Kg. O tratamento com os extratos também causou importante inibição do crescimento tumoral nos camundongos, principalmente o ESC. EB e ESC elevaram a proporção de células inviáveis/viáveis em mais de duas vezes quando comparado ao controle negativo. EB e ESC aumentaram o tempo médio de sobrevida e a concentração de GSH. De acordo com os resultados podemos concluir que ESC apresentou atividade antitumoral mais potente que o extrato bruto. Os resultados obtidos foram favoráveis à validação da utilidade de C. verbenacea como potencial agente antitumoral. Além disso, foi considerado que o método de extração supercrítica pode aprimorar a atividade antitumoral de C. verbenacea, como demonstrado com os resultados apresentados acima, uma vez que estes efeitos provavelmente se devam à presença de ?-humuleno e ?-cariofileno presente nos extratos, especialmente no extrato supercrítico. Também a partir dos resultados obtidos podemos supor que um possível mecanismo de ação antitumoral dos extratos possa ser a redução da expressão da COX-2, o que poderia levar a um bloqueio da sobrevivência celular e indução da apoptose
