1,720,983 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Trastuzumab treatment together with miR-770-5p modulates AKT and ERK expression level.
No full textmiR-770-5p mimic was transfected to BT-474 cells and; total and phoshorylated AKT and ERK protein levels were analyzed by Western blot 72 h after transfection. Combination of trastuzumab with miR-770-5p mostly affected pERK and pAKT levels in BT-474 cells (n = 3, *p<0.05).</p
miR-770-5p regulates motility and invasion in BT-474 and SK-BR-3 cells.
No full text(a) The rate of motility was assessed by wound-healing assay. Wound closure was observed in scrambled control-transfected cells in a time-dependent manner while miR-770-5p mimic transfected cells lost their motility (n = 2, *p(b) Cell invasion analysis was performed by xCELLigence real-time cell analyzer measuring impedance-based signals. Cell invasion capacity decreased in both of the cells transfected with miR-770-5p mimic compared to scrambled control-transfected cells (n = 2, **p<0.0001).</p
miR-770-5p overexpression downregulated HER2 and increased the effect of trastuzumab.
No full text(a) The protein expression level of HER2 was slightly diminished in both cell lines following miR-770-5p transfection n = 3, p(b) Combinational treatment of cells with trastuzumab and miR-770-5p decreased cell viability in BT-474 and SK-BR-3 cells (n = 3, *p(c) (**p<0.05).</p
Lower expression of miR-770-5p is associated with tumor samples.
No full textExpression level of miR-770-5p was downregulated in tumor samples compared to normal samples (One-way ANOVA; p<0.00005621, f = 10.44).</p
Construction of miRNA-miRNA networks revealing the complexity of miRNA-mediated mechanisms in trastuzumab treated breast cancer cell lines.
No full textTrastuzumab is a monoclonal antibody frequently used to prevent the progression of HER2+ breast cancers, which constitute approximately 20% of invasive breast cancers. microRNAs (miRNAs) are small, non-coding RNA molecules that are known to be involved in gene regulation. With their emerging roles in cancer, they are recently promoted as potential candidates to mediate therapeutic actions by targeting genes associated with drug response. In this study we explored miRNA-mediated regulation of trastuzumab mechanisms by identifying the important miRNAs responsible for the drug response via homogenous network analysis. Our network model enabled us to simplify the complexity of miRNA interactions by connecting them through their common pathways. We outlined the functionally relevant miRNAs by constructing pathway-based miRNA-miRNA networks in SKBR3 and BT474 cells, respectively. Identification of the most targeted genes revealed that trastuzumab responsive miRNAs favourably regulate the repression of targets with longer 3'UTR than average considered to be key elements, while the miRNA-miRNA networks highlighted central miRNAs such as hsa-miR-3976 and hsa-miR-3671 that showed strong interactions with the remaining members of the network. Furthermore, the clusters of the miRNA-miRNA networks showed that trastuzumab response was mostly established through cancer related and metabolic pathways. hsa-miR-216b was found to be the part of the most powerful interactions of metabolic pathways, which was defined in the largest clusters in both cell lines. The network based representation of miRNA-miRNA interactions through their shared pathways provided a better understanding of miRNA-mediated drug response and could be suggested for further characterization of miRNA functions
Trastuzumab and Tamoxifen responsive miRNAs were identified by qRT-PCR Array.
No full text(a) HER2+ and ER+ BT-474 cells were treated with both tamoxifen and trastuzumab while ER+ MCF-7 cells and HER2+ SK-BR-3 cells were only tretated with tamoxifen and trastuzumab respectively. There was only one common responsive miRNA among upregulated miRNAs; 11 miRNAs were commonly downregulated in all cell lines. The common responsive miRNAs are listed in b.</p
- …
