1,721,023 research outputs found
Interaction of genetic drift and adaptive evolution in endemic plant species.
No full textInteraction of genetic drift and adaptive evolution in endemic plant specie
Isolation of Ty1-copia putative LTR sequences and their use as a tool to analyse genetic diversity in Olea europea
No full textA DNA fragment putatively belonging to Ty1-copia retrotransposons was isolated from genomic DNA of Olea europaea L., by PCR, using degenerated primers deduced from sequence databases. This fragment includes portions of reverse transcriptase and RNAseH of a copia-like element and has been extended, using chromosome walking techniques, to retrotransposon ends, i.e. the long terminal repeats (LTRs). Ten putative LTR sequences were isolated, and sequence analysis showed that they belong to three different copia subgroups. Putative LTR sequences were evaluated in slot blot hybridisations as to their copy number: only three sequences (Oec04, Oec06 and Oec07), belonging to the same subgroup, were shown to be middle repetitive, with a redundancy ranging from 2.0 × 103 to 2.4 × 103 copies per genome. Southern blot experiments using Oec06 as probe evidenced hybridisation patterns with many weak bands and heavy background smear, indicating genomic interspersion of related sequences. Oec06 was also used to design a primer and to generate REtroelement Microsatellite Amplified Polymorphism (REMAP) molecular markers to distinguish different cultivars of Olea europaea. A total of 108 bands, of which 100 were polymorphic, were obtained. Genetic similarities among cultivars were calculated according to Simple Matching Similarity Index and used to build a dendrogram based on the UPGMA. A comparison between polymorphism based only on retrotransposon sequences and on inter-simple-sequence-repeats (ISSR) showed that polymorphism associated with the former was much higher than that shown with the latter. Our results demonstrate that REMAP markers can be useful in evaluating Olea europaea genetic diversity and in classifying cultivars. © 2006 Springer Science+Business Media B.V
Use of Ty1/Copia retrotransposons to study genetic diversity in olive (Olea europaea L.) cultivars.
No full textUse of Ty1/Copia retrotransposons to study genetic diversity in olive (Olea europaea L.) cultivars
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characterization of HACRE1, a complete Copia retrotransposon of sunflower (Helianthus annuus L.)
No full textCharacterization of HACRE1, a complete Copia retrotransposon of sunflower (Helianthus annuus L.)
Sequence and annotation of three genomic loci of sunflower (Helianthus annuus L.).
No full textSequence and annotation of three genomic loci of sunflower (Helianthus annuus L.)
Computational analysis of LTR-retrotransposons dynamics in Populus trichocarpa
No full textComputational analysis of LTR-retrotransposons dynamics in Populus trichocarpa. Abstract P49
A sequence analysis of genes involved in drought stress in cultivated sunflower and in the Helianthus genus
No full textA survey of LTR-retrotransposons in the Populus trichocarpa genom
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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