1,721,039 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
I geni istonici ed un gene per l'rRNA 5S sono codificati nella stessa unità ripetuta in tandem nel genoma di un crostaceo isopode.
Organization, nucleotide sequence and chromosomal mapping of a tandemly repeated unit containing the four core histone genes and a 5S rRNA gene in an isopod crustacean species.
Abstract: A tandemly repeated unit of 6553 bp containing a copy of the four core histone genes H2B, H2A, H3, and
H4, and also a 5S rRNA gene, was amplified by PCR from genomic DNA of the isopod crustacean Asellus aquaticus.
The linkage between 5S rRNA genes and histone genes has been so far observed in only one other organism, the
anostrac crustacean Artemia salina. The gene cluster was cloned and sequenced. The histone genes, in their 3¢ flanking
region, have the interesting feature of possessing two different mRNA termination signals, the stem-loop structure and
the AATAAA sequence. A part of the PCR product was used as a probe in FISH experiments to locate the gene cluster
on an inter-individually variable number of chromosomes from 6 to 12 per diploid cell, always in a terminal position
and never associated with the heterochromatic areas. Fluorescence in situ hybridization (FISH) was also performed on
preparations of released chromatin and the reiteration level of the gene cluster was determined as approximately 200–
300 copies per haploid genome.
I.F.: 2,2
5S rRNA and U1 snRNA genes, a new linkage type in the genome of a crustacean that has three different tandemly repeated units containing 5S rDNA sequences.
We investigated the 5S ribosomal RNA (rRNA) genes of the isopod crustacean Asellus aquaticus. Using
PCR amplification, three different tandemly repeated units containing 5S rDNA were identified. Two of the three sequences
were cloned and sequenced. One of them was 1842 bp and presented a 5S rRNA gene and a U1 small nuclear
RNA (snRNA) gene. This type of linkage had never been observed before. The other repeat consisted of 477 bp and
contained only an incomplete 5S rRNA gene lacking the first eight nucleotides and a spacer sequence. The third sequence
was 6553 bp long and contained a 5S rRNA gene and the four core histone genes. The PCR products were
used as probes in fluorescent in situ hybridization (FISH) experiments to locate them on chromosomes of A. aquaticus.
The possible evolutionary origin of the three repeated units is discussed.
I.F.: 2.2
Primary cutaneous leiomyosarcoma: a clinicopathological and immunohistochemical study of 7 cases
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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