169,977 research outputs found
Study of the potential of retinal detachment in the human eye in vivo to generate ciliary epithelium derived retinal progenitor cells for transplantation in the porcine eye
No full textIn base ai dati presenti in letteratura il progetto di ricerca programmato “Proliferative Vitreo Retinopathy (PVR): Involvement of Ciliary Body Stem Cells” prevedeva lo studio della sostanza vitreale prelevata durante gli interventi di chirurgia vitreo-retinica in pazienti selezionati con lo scopo di
1. Identificare le cellule staminali del corpo ciliare (CB) e verificare il loro coinvolgimento nella processo a cascata della PVR.
2. Identificazione delle categorie di cellule al margine ciliare e dei fattori chemiotattici e di crescita nel contesto del vitreo.
La ricerca ha avuto inizio due anni dopo dalla sua pianificazione poiché eravamo impossibilitati ad acquistare il materiale necessario al suo espletamento secondariamente alla riduzione ed al ritardo del finanziamento economico.
Pertanto a seguito di ulteriori avanzamenti della ricerca in campo internazionale sulla differenziazione e identificazione delle cellule staminali nel bulbo oculare e al fine di ridurre le spese abbiamo semplificato il progetto inizialmente pianificato.
Per eseguire uno studio ripetibile e riproducibile, abbiamo cercato in particolare di produrre una differenzazione cellulare nel corpo ciliare del porcino determinando. È stato scelto il porcino, animale il cui bulbo oculare e strutturalmente molto vicino a quello dell’uomo la lesione è stata compatibile a quella dell’occhio umano al fine di poter eseguire un successivo esame istologico.
Cioè abbiamo ipotizzato di poter determinare dentro l’occhio malato di un paziente come la DM una lesione accettabile al fine per stimolare la produzione di cellule staminali per poterle impiantare successivamente in quello stesso paziente, eliminando in questo maniera i problemi legati al incompatibilità I risultati degli esami di immunoistochimica, eseguiti in questa prima fase del nostro studio, non hanno evidenziato la proliferazione di nessuna linea cellulare dalla lesione indotta al corpo ciliare del porcino.
I dati scaturiti dalle prime osservazione suggeriscono che probabilmente la presenza delle cellule staminali osservate nel bulbo dell’uomo durante la vitrectomia alla base del corpo vitreo, vicino al margine del corpo ciliare abbiano avuto origine non dal danno ciliare bensì da un danno e/o perdita neuronale.
Supportati da questa ipotesi, anche dagli studi internazionali, il nostro progetto prevede modificando i protocolli iniziali (vitrectomia) necessita d tempi tecnici medio-lunghi per riprodurre nei porcini tutte quelle condizioni di vitrectomizzati. In particolare gli animali, non prima di 3 settimane dalla lesione sperimentale provocata nella retina vengono sottoposti ad una vitrectomia e per comprovare le nostre ipotesi dobbiamo aspettare i risultati dell’istologia.
Al fine di comprovare le supposizione ipotizzate è necessario eseguire e continuare tutti gli studi prima previsti secondo il protocollo di ricerca.
I primi risultati scaturiti dalla presente ricerca sono stati pubblicati nell’articolo
Claude Boscher & Didier Schmidt-Morand & Delphine Holopherne & Olivier Gauthier Experimental vitreoretinal surgery in porcine eyes. Graefes Arch Clin Exp Ophthalmol. 2012 Jan 6, in allegato, grazie alla contributo iniziale della IAPB il suo proseguo necessita necessariamente delle tranche successive
Genetic analysis of 38,XX males with genital ambiguities and true hermaphrodites in pigs
No full textInclusive and accessible implementation of telemedicine: Insights from the United Nations international expert roundtable
No full textInvited panelists from different countries, who are actively involved in digital medicine, discussed the current state and future prospects of telemedicine at a roundtable during an international conference. The discussion covered various aspects of telemedicine, including the available technologies and the critical need for comprehensive databases, as well as insights on completed projects and their long-term viability. Our expertise in technology, sustainability, and telemedicine initiatives can be valuable, with the understanding that the ideas expressed can be applied to all fields and situations, while ensuring that equity and equality in their application are paramount to avoid exacerbating existing disparities. The overall aim is to leverage experience to support the successful implementation of new telemedicine endeavors across different healthcare sectors, with a focus on wide access to technology, affordability, digital literacy, and cultural and linguistic inclusivity
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Erratum to "Variation in genotoxic stress tolerance among frog populations exposed to UV and pollutant gradients" [Aquat. Toxicol. 95 (2009) 152-161] (DOI:10.1016/j.aquatox.2009.09.001)
No full textDispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
A Multi-Language Comparison of Influences on Author Verification using Character N-Grams
No full textWe create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
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