1,721,006 research outputs found
Analysis of genetic and molecular events involved in breaking of dormancy in potato tubers
No full text
Applicability of SSR markers to the traceability of monovarietal olive oils.
No full textBACKGROUND: To protect the features and authenticity of food products, the European Commission enforces two certification labels: Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI). EEC Regulation No. 510/2006 imposes criteria for labelling, production and commercialisation of olive oil. Since plant genotype is a major determinant in establishing the PDO and PGI labels, methods to ascertain the varieties present in a batch of olive oil are essential in validating product conformity. The traceability of olive oil can be assessed through simple sequence repeat (SSR) co-dominant markers targeted to specific regions of DNA from olive cultivars.
RESULTS: Twenty-one monovarietal olive oils were analysed with nine nuclear and two shortened SSRs. For each marker the correspondence of allelic profile with the reference cultivar, the reproducibility of profiles in different DNA extractions and the polymorphism information content were determined.
CONCLUSION: The results showed that using a panel of SSR markers such as those described in this paper allows one to make a reliable attribution of an olive oil to a specific cultivar
Isolation and characterization of gene sequences preferentially expressed during breakage of dormancy and germination in seed potato (Solanum tuberosum var. Mona Lisa).
No full textCloning of developmentally regulated genes by means of differential display in Solanum tuberosum.
No full textIsolation of developmentally and environmentally regulated genes in plants by RNA differential display
No full text
The use of "RNA differential display" in identification of developmentally and environmentally regulated genes in higher plants.
No full textYield and amplificability of different DNA extraction procedures for traceability in the dairy food chain.
No full textSeven DNA extraction procedures were compared for their ability to produce good quality DNA if applied to outputs of the dairy food chain. The efficacy and the efficiency of the protocols were tested. PCR and Real-time PCR specific amplification with appropriate bovine primers were used to assess the quality of the genomic DNA extracted with the compared procedures. The results were also evaluated from the economical point of view, in order to determine which of the seven protocols was the simplest, the most reliable and the most affordable for molecular traceability within this important food chain in order to protect producers from unfair competition and consumers from frauds and adulterations
- …
