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    Age-dependent variations of lactic dehydrogensa and creatinine kinase activities in water buffalo calves serum

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    The aim of the study was the evalutation of the electrophoretic patterns of the serum enzymes lactic dehydrogenase and creatine kinase from buffalo calves. Differences were observed in total activities as their relative distribution at ages ranging from 1 to 10 weeks. The results suggest that isoenzymatic separation and characterization if LDH and CK in relation to the various tissues can significantly contribute to the diagnosis of diseases which are linked to tissue damage

    Evaluation of buffalo colostrum quality by estimation of enzyme activity levels

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    A study to evaluate the value and potential use of colostral enzymes as markers for the evaluation of buffalo colostrum quality was conducted. The enzymes γ-glutamyltransferase (GGT), lactic dehydrogenase (LDH), and alkaline phosphatase (ALP) in buffalo's colostrum were measured spectrophotometrically, and their activities were correlated with the gamma-globulin content. Gamma-globulin concentration was determined following the electrophoretic separation of the colostral proteins and quantified with a densitometer. Colostrum was obtained from 15 dams, soon after calving. Means, standard deviations, correlation coefficients, and degree of significance were calculated using the general linear model procedure of the Statistical Analysis Systems program. The activity of GGT in the colostrum was the highest, followed by LDH and ALP. A significant correlation (r = 0.86; P < 0.001) was seen between GGT and gamma-globulin concentration in the colostrum, supporting the suggestion of using this enzyme as a marker for the evaluation of colostrum quality

    Evaluation of buffalo colostrum quality by estimation of enzyme activity levels.

    No full text
    A study to evaluate the value and potential use of colostral enzymes as markers for the evaluation of buffalo colostrum quality was conducted. The enzymes gamma -glutamyltransferase (GGT), lactic dehydrogenase (LDH), and alkaline phosphatase (ALP) in buffalo's colostrum were measured spectrophotometrically, and their activities were correlated with the gammaglobulin content. Gamma-globulin concentration was determined following the electrophoretic separation of the colostral proteins and quantified with a densitometer. Colostrum was obtained from 15 dams, soon after calving. Means, standard deviations, correlation coefficients, and degree of significance were calculated using the general linear model procedure of the Statistical Analysis Systems program. The activity of GGT in the colostrum was the highest, followed by LDH and ALP. A significant correlation (r = 0.86; P < 0.001) was seen between GGT and gamma-globulin concentration in the colostrum, supporting the suggestion of using this enzyme as a marker for the evaluation of colostrum quality

    GAMMAGLUTAMYLTRANSFERASE AND SERUM PROTEINS IN BUFFALO CALVES FOLLOWING COLOSTRAL INGESTION

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    Serum levels of gamma-glutamyl trasferase (GGT) were determined in buffalo calves for the evaluation of colostral injestion. Levels of GGT were correlated with those of total proteins, albumins and total globulins. Good correlations were seen at day 3 after colostral intake
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