13,851 research outputs found
Protocol: optimised electrophyiological analysis of intact guard cells from arabidopsis
No full textGenetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells
Systems dynamic modeling of a guard cell Cl- channel mutant uncovers an emergent homeostatic network regulating stomatal transpiration
No full textStomata account for much of the 70% of global water usage associated with agriculture and have a profound impact on the water and carbon cycles of the world. Stomata have long been modeled mathematically, but until now, no systems analysis of a plant cell has yielded detail sufficient to guide phenotypic and mutational analysis. Here, we demonstrate the predictive power of a systems dynamic model in Arabidopsis (Arabidopsis thaliana) to explain the paradoxical suppression of channels that facilitate K+ uptake, slowing stomatal opening, by mutation of the SLAC1 anion channel, which mediates solute loss for closure. The model showed how anion accumulation in the mutant suppressed the H+ load on the cytosol and promoted Ca2+ influx to elevate cytosolic pH (pHi) and free cytosolic Ca2+ concentration ([Ca2+]i), in turn regulating the K+ channels. We have confirmed these predictions, measuring pHi and [Ca2+]i in vivo, and report that experimental manipulation of pHi and [Ca2+]i is sufficient to recover K+ channel activities and accelerate stomatal opening in the slac1 mutant. Thus, we uncover a previously unrecognized signaling network that ameliorates the effects of the slac1 mutant on transpiration by regulating the K+ channels. Additionally, these findings underscore the importance of H+-coupled anion transport for pHi homeostasis
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
Interview Excerpt of Mr. Donald M. Crawford, Sr.
No full text(1948-2018) Donald Mitchell Crawford, Sr. (“DC”), musician, author, and educator, was born on May 24, 1948, and was a lifelong resident of Birmingham, AL. His father and mother owned and operated “C & S Charter Tours Bus Company,” the first black-owned bus company in the state of Alabama. Crawford was a 1966 graduate of Western Olin High School in Birmingham, AL. Crawford was an outstanding drum major, playing first chair alto saxophone under the tutelage of the late Amos F. Gordon, Sr. After high school, he received a music scholarship to Alabama State University (ASU) where he earned both his Bachelor’s and Master’s Degrees in Music Education and was later inducted into the “School of Music Hall of Fame” at ASU. He was the youngest ever inducted into the Alabama Jazz Hall of Fame. He was a veteran of the U.S. Army and performed with the 291 st and 283rd Army Band in Fort Bennett, GA. DC was Band Director at Jackson Olin High School and taught in the Birmingham School System for over thirty-five years. His love for music and performing lead him after his retirement to serve as Band Director for Miles College.
Crawford is the author of “The Wheels of the Birmingham Civil Rights Movement.” This book chronicles the lives of his late father and mother, Worcy and Christine Pride Crawford, and the role he and the company played in the Birmingham Civil Rights Movement
Interesting anecdotes, memoirs, allegories, essays, and poetical fragments, [electronic resource] : tending to amuse the fancy, and inculcate morality. By Mr. Addison.
No full textMr. Addison is a pseudonym.On p. 1: Anecdote of Cromwell.Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
Interesting anecdotes, memoirs, allegories, essays, and poetical fragments, [electronic resource] : tending to amuse the fancy, and inculcate morality. By Mr. Addison.
No full textMr. Addison is a pseudonym.P. 1 contains an 'Anecdote' beginning "A certain nobleman, .. ".Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
Interesting anecdotes, memoirs, allegories, essays, and poetical fragments, [electronic resource] : tending to amuse the fancy, and inculcate morality. By Mr. Addison.
No full textMr. Addison is a pseudonym.On p. 1: Anecdote of Doctor Young.Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
Mr Alain Elkann Author and Journalist Italian Republic
No full textVisit by Mr Alain Elkann Author and Journalist Italian Republi
Letter from Misao Miyakawa to Mr. [John Victor] Carson, September 27, 1942
No full textThis letter acknowledges receipt of an affidavit, presumably for Mikoto Miyakawa, see Items csudh_rsp_0684 and csudh_rsp_0685. The author expresses very sincere gratitude for Mr. Carson's kindness
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