63,893 research outputs found
Protocol: optimised electrophyiological analysis of intact guard cells from arabidopsis
No full textGenetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells
Voigtländer’s Tierkalender 1914, Mit 348 Tierbildern nach Naturaufnahmen, Zeichnungen, Kunstwerken usw. Als Abreißkalender eingerichtet. Für jeden Tag ein ganzes Blatt. Preis M 2.80, Durch jede Buchhandlung zu beziehen. Prospekt gratis von R. Voigtländer’s Verlag, Leipzig
No full textVOIGTLÄNDER’S TIERKALENDER 1914, MIT 348 TIERBILDERN NACH NATURAUFNAHMEN, ZEICHNUNGEN, KUNSTWERKEN USW. ALS ABREISSKALENDER EINGERICHTET. FÜR JEDEN TAG EIN GANZES BLATT. PREIS M 2.80, DURCH JEDE BUCHHANDLUNG ZU BEZIEHEN. PROSPEKT GRATIS VON R. VOIGTLÄNDER’S VERLAG, LEIPZIG
Voigtländer’s Tierkalender 1914, Mit 348 Tierbildern nach Naturaufnahmen, Zeichnungen, Kunstwerken usw. Als Abreißkalender eingerichtet. Für jeden Tag ein ganzes Blatt. Preis M 2.80, Durch jede Buchhandlung zu beziehen. Prospekt gratis von R. Voigtländer’s Verlag, Leipzig ( -
Systems dynamic modeling of a guard cell Cl- channel mutant uncovers an emergent homeostatic network regulating stomatal transpiration
No full textStomata account for much of the 70% of global water usage associated with agriculture and have a profound impact on the water and carbon cycles of the world. Stomata have long been modeled mathematically, but until now, no systems analysis of a plant cell has yielded detail sufficient to guide phenotypic and mutational analysis. Here, we demonstrate the predictive power of a systems dynamic model in Arabidopsis (Arabidopsis thaliana) to explain the paradoxical suppression of channels that facilitate K+ uptake, slowing stomatal opening, by mutation of the SLAC1 anion channel, which mediates solute loss for closure. The model showed how anion accumulation in the mutant suppressed the H+ load on the cytosol and promoted Ca2+ influx to elevate cytosolic pH (pHi) and free cytosolic Ca2+ concentration ([Ca2+]i), in turn regulating the K+ channels. We have confirmed these predictions, measuring pHi and [Ca2+]i in vivo, and report that experimental manipulation of pHi and [Ca2+]i is sufficient to recover K+ channel activities and accelerate stomatal opening in the slac1 mutant. Thus, we uncover a previously unrecognized signaling network that ameliorates the effects of the slac1 mutant on transpiration by regulating the K+ channels. Additionally, these findings underscore the importance of H+-coupled anion transport for pHi homeostasis
PROTEIN SECRETION THROUGH THE DEFAULT PATHWAY IS DIFFERENT FROM THE CONSTITUTIVE SECRETION OF CELL WALL POLYSACCHARIDES.
No full textAnion channel sensitivity to cytosolic organic acids implicates a central role for oxaloacetate in integrating ion flux with metabolism in stomatal guard cells
No full textStomatal guard cells play a key role in gas exchange for photosynthesis and in minimizing transpirational water loss from plants by opening and closing the stomatal pore. The bulk of the osmotic content driving stomatal movements depends on ionic fluxes across both the plasma membrane and tonoplast, the metabolism of organic acids, primarily Mal (Imitate), and its accumulation and loss. Anion channels at the plasma membrane are thought to comprise a major pathway for Mal efflux during stomatal closure, implicating their key role in linking solute flux with metabolism. Nonetheless, little is known of the regulation of anion channel current (I(Cl)) by cytosolic Mal or its immediate metabolite OAA (oxaloacetate). In the present study, we have examined the impact of Mal, OAA and of the monocarboxylic acid anion acetate in guard cells of Vicia faba L. and report that all three organic acids affect I(Cl), but with markedly different characteristics and sidedness to their activities. Most prominent was a suppression of I(Cl) by OAA within the physiological range of concentrations found in vivo. These findings indicate a capacity for OAA to co-ordinate organic acid metabolism with I(Cl), through the direct effect of organic acid pool size. The findings of the present study also add perspective to in vivo recordings using acetate-based electrolytes
Thermal expansion anomalies of R(Fe, M)(12) (R=Y, Nd; M=Mo and Si)
No full textStructural and thermal-expansion anomaly studies on R(Fe,M)(12) (R=Nd and and Y, M=Mo and Si) compounds were performed by x-ray diffraction. Mo atoms occupy the 8i site. While Si atoms occupy the 8f and 8j sites but not the 8i site. Thermal-expansion anomaly shows only in ab plane in the Mo compounds, while becomes very weak and along with only the c axis in the Si compounds. The anomaly was attributed to the contribution of the interactions of short Fe-Fe distances similar to the previous explanation on other R-Fe intermetallics and that of other strongly positive interactions such as 8j-8j. (c) 2005 American Institute of Physics.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000230168300025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Physics, AppliedSCI(E)EICPCI-S(ISTP)
Methods of sampling and analysis for sulphur dioxide, oxides of nitrogen and particulate matter in the exhaust gases of large combustion plant. EUR 10645 EN. Environment and quality of life
No full textLetter from Thomas R. Bodine, American Friends Service Committee Seattle office, to Mary M. Kimber, May 25, 1942
No full textLetter from Thomas R. Bodine to Mary M. Kimber, asking Kimber to visit individuals from the Puget Sound area incarcerated at Pinedale Assembly Center: Rev. Daisuke Kitigawa, Waichi Oyanagi, Chisako Higuchi, Mutsuo Hasiguchi and Mrs. Matsuoka, Makato Kobukata, the Hirabayashi family, and Violet Yokoyama. A note in pencil at the top of the page: "Burcham." A response letter from Grace and Calvin Coke to Thomas R. Bodine is found in item: chs_ms840_0306.Personal correspondence, organizational records, government documents, publications, and other papers created or collected by Joseph R. Goodman documenting the forced removal and incarceration of Japanese Americans during World War II, as well as organized resistance to incarceration. Included in the collection are records of the Japanese Young Men's Christian Association and the Japanese American Citizens' League in San Francisco, including papers of the Japanese YMCA's executive secretary Lincoln Kanai; Sakai family papers; Goodman's correspondence to and from Japanese American incarcerees, organizations opposing forced removal and incarceration of Japanese Americans, the War Relocation Authority, and others; publications, photographs, and ephemera from the Topaz Relocation Center, where Goodman taught high school; War Relocation Authority records and publications; and newspaper clippings, pamphlets, and reports about forced removal and incarceration created by various government, religious, and civic organizations, in California and nationwide
The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidences from competition with its cytosolic domain.
No full textThe abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: Evidence from competition with its cytosolic domain
No full textSyntaxins and other SNARE proteins are crucial for intracellular vesicle trafficking, fusion, and secretion. Previously, we isolated the syntaxin-related protein NtSyr1 (NtSyp121) from tobacco in a screen for abscisic acid-related signaling elements, demonstrating its role in determining the abscisic acid sensitivity of K+ and Cl- channels in stomatal guard cells. NtSyr1 is localized to the plasma membrane and is expressed normally throughout the plant, especially in root tissues, suggesting that it might contribute to cellular homeostasis as well as to signaling. To explore its functions in vivo further, we examined stably transformed lines of tobacco that expressed various constructs of NtSyr1, including the full-length protein and a truncated fragment, Sp2, corresponding to the cytosolic domain shown previously to be active in suppressing ion channel response to abscisic acid. Constitutively overexpressing NtSyr1 yielded uniformly high levels of protein (>10 times the wild-type levels) and was associated with a significant enhancement of root growth in seedlings but not with any obvious phenotype in mature, well-watered plants. Similar transformations with constructs encoding the Sp2 fragment of NtSyr1 showed altered leaf morphology but gave only low levels of Sp2 fragment, suggesting a strong selective pressure against plants expressing this protein. High expression of the Sp2 fragment was achieved in stable transformants under the control of a dexamethasone-inducible promoter. Sp2 expression was correlated positively with altered cellular and tissue morphology in leaves and roots and with a cessation of growth in seedlings. Overexpression of the full-length NtSyr1 protein rescued the wild-type phenotype, even in plants expressing high levels of the Sp2 fragment, supporting the idea that the Sp2 fragment interfered specifically with NtSyr1 function by competing with NtSyr1 for its binding partners. To explore NtSyr1 function in secretion, we used a green fluorescent protein (GFP)-based section assay. When a secreted GFP marker was coexpressed with Sp2 in tobacco leaves, GFP fluorescence was retained in cytosolic reticulate and punctate structures. In contrast, in plants coexpressing secreted GFP and NtSyr1 or secreted GFP alone, no GFP fluorescence accumulated within the cells. A new yellow fluorescent protein-based secretion marker was used to show that the punctate structures labeled in the presence of Sp2 colocalized with a Golgi marker. These structures were not labeled in the presence of a dominant Rab1 mutant that inhibited transport from the endoplasmic reticulum to the Golgi. We propose that NtSyr1 functions as an element in SNARE-mediated vesicle trafficking to the plasma membrane and is required for cellular growth and homeostasis
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