1,721,252 research outputs found

    Novel staining procedures for whole mount preparations of greater wax moth (Galleria mellonella) embryos: toluidine blue-rhodamine B, plus calcofluor white

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    Although hematoxylin and eosin (H & E) staining of sectioned embryonic insect material is widely used, it is time-consuming and may not provide sufficient information. We evaluated new staining procedures for embryonic whole mounts of the greater wax moth, Galleria mellonella. We compared a combination of toluidine blue and rhodamine B (TB-RB) to H & E; we also investigated calcofluor white (CFW) staining. TB-RB staining produced staining similar to H & E. TB-RB staining was less time-consuming and improved visualization of the blastoderm and its differentiation into the germ disk and serosa membrane. CFW enhanced details of mitosis in nuclei post-fertilization and stained the primary serosal membrane. Staining of whole mounts with TB-RB and CFW enabled embryonic staging that was more rapid, convenient and effective than the routine approach using H & E and fluorescent probes

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Algorithms for operational decision-making: An absorptive capacity perspective on the process of converting data into relevant knowledge

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    The organisational mechanisms through which algorithms can be exploited in the process of converting data into relevant knowledge for operational decision-making have not yet been fully investigated from an absorptive capacity perspective. Previous studies underlined a rise in new digital specialised roles, but they said little about how the organisational knowledge and structures should be redesigned to take advantage of these data-rich operational environments. In this article, we present the findings of a case study on the way algorithms can be exploited in the electrical sector to shed light on these issues. We then develop a framework to theorise how the organisational mechanisms associated with absorptive capacity influence the way algorithms can be exploited to convert data into relevant knowledge for operational decision-making. Our emerging framework reveals that to convert data into relevant knowledge for operational decision-making, the involvement of line employees and liaison roles are required to introduce system-level knowledge that algorithms are able to capture less effectively. Additionally, more formalisation is needed in operational work to ensure the quality of the data that feed such algorithms. Finally, socialisation tactics facilitate the convergence between the knowledge produced from algorithms and the experiential knowledge of line employees

    Temporal reference of the mnemonic sources of dreams.

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    The aim of this experiment was to investigate the temporal reference of elements which served as the mnemonic basis for dreams. Free associations with REM and Sleep Onset dreams were collected to determine the quality of the memories presumably activated during the producrion of the two types of dream. Associations referring to particular episodes of the dream- er's life were classified, according to their temporal dating, into Day residues, Recent residues, Remote residues. The hypothesis that there are differences in the temporal reference of the mnemonic sources of REM and sleep onset dreams has been confirmed. Day residues are significantly more associated with sleep onset than REM dreams. Moreover, in sleep onset, Day residues are significantly more frequent than Remote residues, while this is not true for REM dreams. Day residues are more easily available to sleep onset than REM dreaming because of the recent encoding which characterizes this kind of memory traces. In both sleep phases recent residues are more frequently used in constructing the dream
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