1,721,008 research outputs found
Electrophoretic separation of purified myelin: a method to improve the protein pattern resolving.
No full textMyelin sheath is a lipid-rich membrane, consisting of 70% lipid and 30% proteins, that is involved in physiological and pathological processes. For this reason its protein composition has been often investigated, principally by two-dimensional electrophoresis; however, the consistent lipid content makes it difficult to obtain good proteins separation. To improve the resolution of myelin proteins in a denaturing monodimensional gel electrophoresis, we examined several mixtures for the denaturation of the sample, utilizing different detergents and reducing agents. The definition of the protein pattern was analyzed by both "Blue Silver" Coomassie staining and Western Blot analysis against myelin basic protein, one of the most represented myelin proteins. The best resolution is observed when the sample was incubated with a mixture containing 1.25% dithiothreitol, 4 M urea, and 1% dodecyl maltoside or 1 % 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, prior to addition of denaturing agents. In conclusion, this work describes a novel method to improve the separation of myelin proteins in a monodimensional gel electrophoresis. It may be also useful for investigating other lipid-rich samples
Energetic metabolism of myelinated axons: a correlation among extramitochondrial atp production in myelin and the sheath development.
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Extramitochondrial oxidative phosphorylation: characterization and quantification of Cardiolipin in myelin sheath.
No full textEnergetic metabolism of myelinated axons: a correlation among extramitochondrial ATP production in myelin and the sheath development.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
New insights in neurodegeneration: Extra-mitochondrial oxidative phosphorylation in nervous system.
No full textExtramitochondrial oxidative phosphorylation in myelin sheath: reactive oxygen species production and axonal degeneration in demyelinating diseases.
No full textExtramitochondrial oxidative phosphorylation in myelin sheath: reactive oxygen species production and axonal degeneration in demyelinating diseases.
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