6 research outputs found
Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry
No full textWe studied the occurrence of T-cell subpopulations for patients with B-cell chronic lymphocytic leukemia. The CD8+ population was divided into CD8+ suppressor (CD8a+) and CD8+ cytotoxic (CD8b+) lymphocytes using difference in orthogonal light scattering. Average CD4+/CD8+ratios determined for all patients were decreased. For individual patients this sometimes was not true. In contrast CD4+/CD8a+ ratios were markedly increased in all individual patients. The CD8+ lymphocytes appeared to consist mainly of CD8b+lymphocytes. Moreover the CD8b+/CD8+ ratio correlated with clinical stage: untreated patients (stage 0 of Rai) have smaller CD8b+/CD8+ ratios than patients with advanced stages of Rai
The fresco decoration by Felix Anton Scheffler in the church St. George and St. Martin in Martínkovice
No full textThis bachelor thesis focuses on the fresco decoration in the Church of St. George and St. Martin in Martínkovice, which was created by Felix Anton Scheffler in 1748. Firstly, the thesis deals with history and typology of the church, followed by the description of works by the architect Martin Allio. The greatest part of the thesis is devoted to the painter Felix Anton Scheffler and his works reaching from Silesia to Bohemia. The author mentions Felix Anton Scheffler´s teachers - Cosmas Damian Asam and Johann Christoph Grooth. The main part of the thesis is focused on frescos which are subject to an iconographic and formal analysis and also focuses on restoration work. Scheffler´s works are put into the context of baroque paintings in Bohemia. The last part concentrates on searching patterns that are used in the fresco in the Church of St. George and St. Martin in Martínkovice. The author compares frescos from Martínkovice with Asam´s other works from the period between 1720 and 1725
Experimental and model investigations of bleaching and saturation of fluorescence in flow cytometry
No full textExperimental and model investigations of bleaching and saturation of fluorescence in flow cytometry Doornbos, R.M.P.; de Grooth, B.G.; Greve, J. Published in: Cytometry DOI: 10.1002/(SICI) 1097-0320(19971101) General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. We used the model to determine the sensitivity of our flow cytometer and to calculate the optimum conditions for the detection of APC. The results show that in principle a single APC molecule on a cell can be detected in the presence of background, i.e., autofluorescence and Raman scattering by water
Evaluation of Membrane Integrity Monitoring Methods for Hollow Fiber Nanofiltration Membranes: Applicability in Gray Water Reclamation Systems
No full textSource-separated gray water reclamation using nanofiltration as an advanced post-treatment option has received substantial interest in meeting the growing water demand. During reclamation, membrane integrity is crucial to ensure the water’s safety. This study evaluated several chemical and novel microbial indicators as indirect membrane integrity-monitoring methods for hollow fiber nanofiltration membranes in reclamation schemes. Under normal conditions, high retention of divalent ions and organic matter and near-complete removal of Escherichia coli (E. coli) were observed. Limited removal of the antibiotic gene (ARG) tetO was observed due to low feed concentrations and a higher detection limit (LOD). While 16S rRNA and ARG sul1 were not limited by their LODs, lower removals were observed, most likely due to free-floating DNA passing through the membranes. A broken fiber in a pilot-scale module reduced organic matter and microorganism removal substantially, while flux and ion rejection remained similar. Predictions made using the observed results and a previously proposed model allowed for the evaluation of the selected methods in upscaled reclamation systems. Based on these results, it was concluded that microorganisms could be employed as indicators in indirect membrane integrity-monitoring methods in large-scale reclamation schemes, while UV254nm absorbance (used in organic matter determination) could be a viable solution in pilot-scale systems.BT/Environmental Biotechnolog
Additional file 1 of Effect of lung ultrasound-guided fluid deresuscitation on duration of ventilation in intensive care unit patients (CONFIDENCE): protocol for a multicentre randomised controlled trial
No full textAdditional file 1: Supplement I. Supplemental figure 1. Lung regions used for lung ultrasound examination. A. supine position. Each hemithorax is divided in to six regions: upper and lower parts of the anterior, lateral and posterior chest wall. B: prone position. Each hemithorax is divided in to six regions: upper and lower parts of the posterior, lateral and anterior chest wall. Adapted from Heldeweg et al. with permission of the author (1). Supplement II. Definitions of all endpoints. Supplement III. Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 Checklist. Supplement IV. A list of all data collected
Co-infection and ICU-acquired infection in COIVD-19 ICU patients: a secondary analysis of the UNITE-COVID data set
No full textBackground: The COVID-19 pandemic presented major challenges for critical care facilities worldwide. Infections which develop alongside or subsequent to viral pneumonitis are a challenge under sporadic and pandemic conditions; however, data have suggested that patterns of these differ between COVID-19 and other viral pneumonitides. This secondary analysis aimed to explore patterns of co-infection and intensive care unit-acquired infections (ICU-AI) and the relationship to use of corticosteroids in a large, international cohort of critically ill COVID-19 patients. Methods: This is a multicenter, international, observational study, including adult patients with PCR-confirmed COVID-19 diagnosis admitted to ICUs at the peak of wave one of COVID-19 (February 15th to May 15th, 2020). Data collected included investigator-assessed co-infection at ICU admission, infection acquired in ICU, infection with multi-drug resistant organisms (MDRO) and antibiotic use. Frequencies were compared by Pearson’s Chi-squared and continuous variables by Mann–Whitney U test. Propensity score matching for variables associated with ICU-acquired infection was undertaken using R library MatchIT using the “full” matching method. Results: Data were available from 4994 patients. Bacterial co-infection at admission was detected in 716 patients (14%), whilst 85% of patients received antibiotics at that stage. ICU-AI developed in 2715 (54%). The most common ICU-AI was bacterial pneumonia (44% of infections), whilst 9% of patients developed fungal pneumonia; 25% of infections involved MDRO. Patients developing infections in ICU had greater antimicrobial exposure than those without such infections. Incident density (ICU-AI per 1000 ICU days) was in considerable excess of reports from pre-pandemic surveillance. Corticosteroid use was heterogenous between ICUs. In univariate analysis, 58% of patients receiving corticosteroids and 43% of those not receiving steroids developed ICU-AI. Adjusting for potential confounders in the propensity-matched cohort, 71% of patients receiving corticosteroids developed ICU-AI vs 52% of those not receiving corticosteroids. Duration of corticosteroid therapy was also associated with development of ICU-AI and infection with an MDRO. Conclusions: In patients with severe COVID-19 in the first wave, co-infection at admission to ICU was relatively rare but antibiotic use was in substantial excess to that indication. ICU-AI were common and were significantly associated with use of corticosteroids. Trial registration ClinicalTrials.gov: NCT04836065 (retrospectively registered April 8th 2021). Graphical abstract: [Figure not available: see fulltext.]. © 2022, The Author(s)
