15,022 research outputs found
Ty Matson: Senior Recital, Trombone
No full textThis Senior Honors Project is entitled “Ty Matson: Senior Recital, Trombone.” The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital, with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles, musical eras, and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital, not including intermission, lasted approximately 47 minutes
Ty Matson: Senior Recital , Trombone
No full text"This Senior Honors Project is entitled ""Ty Matson: Senior Recital , Trombone."" The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital , with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles , musical eras , and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital , not including intermission , lasted approximately 47 minutes.
Ty Matson: Senior Recital, Trombone
No full textThis Senior Honors Project is entitled “Ty Matson: Senior Recital, Trombone.” The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital, with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles, musical eras, and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital, not including intermission, lasted approximately 47 minutes
Localisation of TY-TgsGP.
No full text<p>Localisation of TY-tagged TgsGP (red) relative to un-endocytosed FITC-labeled Concanavalin A bound to glycoproteins in the flagellar pocket (green) and DAPI stained nucleus and kinetoplast (blue) in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003686#ppat.1003686-Barrett1" target="_blank">[1]</a> TY-<i>TgsGP</i><sup>−/+</sup><i>T. b. brucei</i> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003686#ppat.1003686-Vincendeau1" target="_blank">[2]</a><i>TbbHbHpR</i><sup>−/+</sup> TY-<i>TgsGP</i><sup>−/+</sup><i>T. b. gambiense</i>. The flagellar pocket (revealed by Concanavalin A and kinetoplast position) is indicated with a white arrow.</p
Systematic Ty mapping.
No full text<p>(A) Mapping strategy. (B) Ty in the <i>PEX25</i>-<i>CAR1</i> intergenic region. (C) Number of SGRP reads supporting each Ty position in SK1. The observed distribution of read frequencies around each of 28 Ty sites is compared to that expected from a Poisson distribution with the same mean (λ = 8.6). (D) Copy number of Tys from different families in SK1 and S288C.</p
HPLC fractionation and surface micellization behavior of polystyrene-b-poly(methyl methacrylate)
No full textA polystyrene-block-poly(methyl methacrylate) (PS-b-PMMA) was fractionated by HPLC, and the morphology of surface micelles formed at the air/water interface was investigated. Individual PS and PMMA blocks were fractionated by normal-phase and reversed-phase HPLC, respectively, into three fractions each to obtain nine fractions of narrower distribution in molecular weight as well as in composition. Although the mother PS-b-PMMA was prepared by anionic polymerization and had a narrow molecular weight distribution as characterized by size exclusion chromatography (Mw = 72.8 kg/mol, M w/Mn = 1.08, PS wt % = 73.7%), the nine fractionated samples showed significant variations in molecular weight (Mw = 63.1-79.5 kg/mol) and in composition (PS wt % = 66.5-76.3%). The fractionated block copolymers formed more uniform surface micelles than the unfractionated mother PS-b-PMMA and exhibited a large variation with respect to the composition. The PS-b-PMMA fraction of the lowest PS content (66.5 wt %) showed circular micelles only. Rod-shaped micelles started to appear with a slight increase in the PS content, but circular and rod-shaped micelles coexist over ca. 10% concentration range and no sharp phase boundary appears to exist. The rod-shaped micelles formed at low PS contents exhibit a structure of linearly connected circular micelles.close292
Evolutionary genomics of transposable elements in Saccharomyces cerevisiae
No full textSaccharomyces cerevisiae is one of the premier model systems for studying the genomics and evolution of transposable elements. The availability of the S. cerevisiae genome led to unprecedented insights into its five known transposable element families (the LTR retrotransposons Ty1-Ty5) in the years shortly after its completion. However, subsequent advances in bioinformatics tools for analysing transposable elements and the recent availability of genome sequences for multiple strains and species of yeast motivates new investigations into Ty evolution in S. cerevisiae.
Here we provide a comprehensive phylogenetic and population genetic analysis of all Ty families in S. cerevisiae based on a systematic re-annotation of Ty elements in the S288c reference genome. We show that previous annotation efforts have underestimated the total copy number of Ty elements for all known families. In addition, we identify a new family of Ty3-like elements related to the S. paradoxus Ty3p which is composed entirely of degenerate solo LTRs. Phylogenetic analyses of LTR sequences identified three families with short-branch, recently active clades nested among long branch, inactive insertions (Ty1, Ty3, Ty4), one family with essentially all recently active elements (Ty2) and two families with only inactive elements (Ty3p and Ty5). Population genomic data from 38 additional strains of S. cerevisiae show that elements present in active clades are predominantly polymorphic, whereas most of the inactive elements are fixed. Finally, we use comparative genomic data to provide evidence that the Ty2 and Ty3p families have arisen in the S. cerevisiae genome by horizontal transfer.
Our results demonstrate that the genome of a single individual contains important information about the state of TE population dynamics within a species and suggest that horizontal transfer may play an important role in shaping the genomic diversity of transposable elements in unicellular eukaryotes
Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinases
No full textAbstract Background Overexpression of Aurora A and B has been reported in a wide range of tumor types, including gastric cancer. Anti-angiogenesis has been considered as an important therapeutic modality in advanced gastric cancer. Here we identified a novel compound TY-011 with promising antitumor activity by targeting mitotic kinases (Aurora A and B) and angiogenic receptor tyrosine kinase (VEGFR2). Methods HTRF® KinEASE™ assay was used to detect the effect of TY-011 against Aurora A, Aurora B and VEGFR2 activities. Docking simulation study was performed to predict the binding mode of TY-011 with Aurora A and B kinases. CCK-8 assay was used to test cell growth. Cell cycle and cell apoptosis was analyzed by flow cytometry. Gastric cancer cell xenograft mouse models were used for in vivo study. TUNEL kit was used to determine the apoptosis of tumor tissues. Immunohistochemistry analysis and HUVEC tube formation assay were performed to determine the anti-angiogenesis ability. Immunofluorescence and western blot were used to test protein expression. Results TY-011 was identified as a potential Aurora A and B inhibitor by HTRF® KinEASE™ assay. It effectively inhibited cellular Aurora A and B activities in a concentration-dependent manner. TY-011 occupied the ATP-binding site of both Aurora A and B kinases. TY-011 demonstrated prominent inhibitory effects on proliferation of gastric cancer cells. TY-011 treatment induced an obvious accumulation of cells at G2/M phase and a modest increase of cells with >4 N DNA content, which then underwent apoptosis. Meaningfully, orally administration of TY-011 demonstrated superior efficacy against the tumor growth in gastric cancer cell xenograft, with ~90% inhibition rate and 100% tumor regression at 9 mg/kg dose, and TY-011 did not affect the body weight of mice. Interestingly, we observed that TY-011 also antagonized tumor angiogenesis by targeting VEGFR2 kinase. Conclusions These results indicate that TY-011 is a well-tolerated, orally active compound that targets mitosis and angiogenesis in tumor growth, and provides strong preclinical support for use as a therapeutic for human gastric cancers
Characterization of poly(ethylene oxide)-b-poly(L-laciide) block copolymer by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
No full textA poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI-TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.X1110sciescopuskc
Un teorema de puntos fijos para operadores de la clase D (a, b)
No full textSea x un espacio normado y K un subconjunto de . La clase D(a,b ) sobre K está formada por todos los operadores T : K → K que satisface la condición.
│Tx - Ty│ ≤ a│x-y│+ b │x- Tx│ + │y- Ty│ para todo x, y en K, donde a, b son números no negativo
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