23,668 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Four and a half LIM protein 1C (FHL1C)
No full textFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
cDNA cloning and mRNA expression of LIM protein gene homologue from the silkworm, Bombyx mori
No full textLIM protein cDNA, from Bombyx mori that contains an open reading frame of 622 bp encoding 94 amino acids, was identified and characterized. The B. mori LIM protein homologue is classified into group 2 LIM proteins that contain glycine-rich LIM domain. B. mori LIM protein mRNA is up-regulated at late embryogenesis and detected in the mid-gut of 5th instar larvae.open
The Nuisance Midges (Diptera: Chironomidae) Of Singapore'S Pandan And Bedok Reservoirs
No full textCranston, P. S., Ang, Y. C., Heyzer, A., Lim, R. B. H., Wong, W. H. (2013): The Nuisance Midges (Diptera: Chironomidae) Of Singapore'S Pandan And Bedok Reservoirs. Raffles Bulletin of Zoology 61 (2): 779-793, DOI: 10.5281/zenodo.535304
Test and teach. Number ninety seven: Granulomatous inflammation and CNS germinoma
No full textB. Amanuel, M. H. T. Lim, G. Scott, P. C. Blumbergs, R. W. Byar
Figure 4. Parascleroderma tetradentica Lim and S in A taxonomic review of the subfamily Pristocerinae (Hymenoptera: Chrysidoidea: Bethylidae) from Korea with descriptions of two new species
No full textFigure 4. Parascleroderma tetradentica Lim and S. Lee sp. nov. (male). (A) whole body; (B) head; (C) mandible and clypeus; (D) antenna; (E) pronotum and mesonotum; (F) propodeum; (G) right forewing; (H) metasoma; (I) subgenital plate in ventral view; (J) genitalia in ventral view (scale bar 1.00 mm for (A); 0.20 mm for (C), (I) and (J); 0.30 mm for (B) and (D)–(F); 0.50 mm for (G) and (H)).Published as part of Lim, Jongok, Terayama, Mamoru, Koh, Sanghyun, Lee, Jongwook & Lee, Seunghwan, 2011, A taxonomic review of the subfamily Pristocerinae (Hymenoptera: Chrysidoidea: Bethylidae) from Korea with descriptions of two new species, pp. 435-460 in Journal of Natural History 45 (7-8) on page 447, DOI: 10.1080/00222933.2010.534191, http://zenodo.org/record/520503
Tytthoscincus temasekensis Grismer, Wood, Lim, Liang 2017
No full text<i>Tytthoscincus temasekensis</i> Grismer, Wood, Lim, Liang 2017 <p> <b>Holotype and type locality:</b> Adult female (ZRC 2.6490) from “Pasir Laba Road, Singapore (N 01°19.526′, E 103°40.212′; 24 m elevation).”</p> <p> <b>Distribution:</b> Known from the type locality, lowland areas in Singapore, and near Tanjung Malim, Perak, Peninsular Malaysia (Grismer <i>et al.</i> 2017b).</p>Published as part of <i>Grismer, L. Lee & Quah, Evan S. H., 2019, An updated and annotated checklist of the lizards of Peninsular Malaysia, Singapore, and their adjacent archipelagos, pp. 230-248 in Zootaxa 4545 (2)</i> on page 242, DOI: 10.11646/zootaxa.4545.2.4, <a href="http://zenodo.org/record/2618805">http://zenodo.org/record/2618805</a>
Fig. 1 in An update to the Inland Fishes of Pulau Tioman, Malaysia
No full textFig. 1. Barbonymus schwanenfeldii (ca. 40 mm SL) in-situ in Sg. Keliling (2014).Published as part of Tan, H. H., Low, B. W., Yeo, Darren C. J. & Lim, Kelvin K. P., 2015, An update to the Inland Fishes of Pulau Tioman, Malaysia, pp. 555-563 in Raffles Bulletin of Zoology 63 on page 556, DOI: 10.5281/zenodo.538660
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