1,720,999 research outputs found
The Debate Concerning the Off-label Prescriptions of Drugs: A Comparison between Italian and U.S. Law
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Il trapianto di rene e la donazione altrustica, dal buon samaritano al compenso pecuniario
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES
Full text linkThis PhD project focuses on the identification, isolation and characterization of new biocatalysts able to generate biologically active molecules with significant enantioselectivity. Through screening, we identified marine strains, from MaCuMBA (Marine Culturable Microorganism for Biotechnological Applications) and BIODEEP (Biotechnologies form the deep) European project collections, which show a marked enantioselectivity on intermediates of molecules of biological interest. Biotransformation substrate range included pramipexole, as main target, but it also embraces other common building blocks for synthetic industrial preparation.
The stereoselective reduction of structurally different ketones using halotolerant marine yeasts (Meyerozyma guilliermondii and Rhodotorula mucilaginosa) was studied using cells grown and bio-converted in seawater. The preparation of valuable chemicals through water-saving (bio)processes based on the direct exploitation of seawater is a significant step towards sustainable biocatalysis. By choosing a suitable strain, high yields and stereoselectivity could be achieved in most cases. Notably, high chemoselectivity and enantioselectivity were observed using R. mucilaginosa in the reduction of aromatic β-ketonitriles, which allowed the recovery of the optically pure corresponding alcohols; notably, reduction with whole cells of yeasts generally give a mixture of undesired products, as observed with M. guilliermondii.
Keto-reduction potential of thirty-three marine bacterium species was checked and afterwards the possibility to convert this substrate directly into the optically pure amine was investigated: marine bacteria were screened to identify transaminase activity. Based on the previous results in terms of halotolerance and transaminase activity, the marine bacterium strain Virgibacillus pantothenticus 21D was selected for the genome sequencing in order to clone and express an ω-transaminase enzyme.
A recombinant non-marine ketoreductase from Pichia glucozyma (KRED1-Pglu) was used for the enantioselective reduction of various cyclic ketones including pramipexole ketone intermediate. Thanks to co-factor recycling system, the purified enzyme showed very promising results.
The soluble expression of a novel omega transaminase from a newly isolated halotolerant marine bacterium Virgibacillus pantothenticus was attained. Despite of several standard methodologies applied, the marine wild-type enzyme was total insoluble in E. coli host and it was satisfactorily solubilized by one single-point mutation, allowing the characterization of the new omega transaminase. The enzyme shows an interesting salt and solvent tolerance, in accordance to its origin and it results particularly active on some interesting building blocks molecules
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dopo l'Unità: paesaggio naturale e urbano, tematiche sociali e religiose
No full textTesto introduttivo alla sezione del catalogo e schede di opere dei due maggiori pittori ferraresi contemporanei di Boldini e Previati: Giuseppe Mentessi ("Venezia, sagrato della basilica di San Marco"; "Panem Nostrum Quotidianum") e Alberto Pisa ("Il ponte di Charing Cross a Londra")
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