37 research outputs found
MULTIOMICS EVALUATION OF THE EFFECT OF EZRIN INHIBITOR IN DIFFUSE LARGE B CELL LYMPHOMA REVEALS DYSREGULATION OF BCR SIGNALING, RHO GTPASE SIGNALING AND APOPTOSIS
No full textDecay of a microsecond seniority 3 isomeric state in Hf 155
No full textExcited states in the neutron-deficient nuclide Hf155 have been investigated in experiments performed at the Accelerator Laboratory of the University of Jyväskylä. The Hf155 nuclei were produced in fusion-evaporation reactions induced by beams of 295 and 315 MeV Ni58 ions bombarding an isotopically enriched Pd102 target and separated using the recoil mass separator MARA. An isomeric state having a half-life of 510(30) ns was discovered and is interpreted as a seniority υ=3, (πh11/22 - νf7/2)27/2- configuration. The γ-ray transitions emitted in the deexcitation of the isomeric state to the ground state were identified and a level scheme was constructed, from which the excitation energy of the isomer was determined to be 2581.5(10) keV. A B(E2) value of 0.45(3) W.u. was deduced for the 105.4 keV transition depopulating the isomeric state. The deduced level scheme and B(E2) value are compared with systematics and shell-model calculations.</p
A multicolour flow cytometric assay for c-MYC protein in B-cell lymphoma
No full textAimDevelop an objective assay to detect c-MYC protein expression using multiparametric flow cytometry (FCM) as an alternative to immunohistochemistry (IHC).Methods57 patient samples and 11 cell line samples were evaluated. Cell suspensions were obtained and c-MYC staining was performed in combination with CD45 and CD19 and, in some samples, CD10. The percentage of c-MYC+ cells by FCM was correlated with the percentage determined by IHC. The relationship between c-MYC protein expression and the presence of ac-MYCgene rearrangement in aggressive and high-grade lymphomas was also assessed.Resultsc-MYC expression by FCM and IHC demonstrated a high degree of correlation in a training set of 33 patient cases, r=0.92, 11 cell line samples, r=0.81 and in a validation set of 24 aggressive and high-grade B-cell lymphomas, r=0.85.c-MYCgene was rearranged by fluorescence in situ hybridisation in 6/9 samples with high c-MYC expression (>40%) by FCM and 6/14 by IHC.ConclusionsWe have developed a reliable multicolour FCM assay to detect c-MYC expression suitable for clinical laboratories that should be helpful to accurately quantify c-MYC expression in B-cell lymphomas.</jats:sec
Saudi Arabian ICU safety and nurses' attitudes
No full textPURPOSE: The purpose of this paper is to examine nurses' attitudes towards safety culture in six Saudi Arabian intensive care units (ICUs). DESIGN/METHODOLOGY/APPROACH: The study is descriptive with a cross-sectional design. The Safety Attitude Questionnaire (SAQ)-ICU version was distributed and 216 completed questionnaires were returned. FINDINGS: The findings provide a basis for further research on Saudi Arabian ICU safety culture. This study showed that the SAQ-ICU can be used to measure safety climate to identify areas for improvement according to nurse attitudes and perceptions. Findings indicate that ICU safety culture is an important issue that hospital managers should prioritise. PRACTICAL IMPLICATIONS: The SAQ-ICU questionnaire, used to measure safety climate in Saudi Arabian ICUs, identifies service strengths and improvement areas according to attitudes and perceptions. ORIGINALITY/VALUE: To the knowledge, this is the first study to use SAQ to examine nurses' safety culture attitudes in Saudi Arabian ICUs. The present findings provide a baseline and further details about Saudi Arabian ICU safety. Study participants represented nine nationalities, indicating the nursing workforce's diversity, which is expected to continue in the future. Such a nursing cultural heterogeneity calls for further studies to examine and evaluate attitudes and values to improve ICU safety culture
Long-Range PCR and Nanopore Sequencing Enables High-Throughput Detection of TCF4 Trinucleotide Repeat Expansions in Fuchs Endothelial Corneal Dystrophy
No full textIntroduction
Trinucleotide repeat expansion in CTG18.1, in intron 2 of TCF4 (MIM *602272, #613267), is the main cause of Fuchs endothelial corneal dystrophy (FECD), accounting for around 75% of cases in Caucasians. CTG18.1 repeat expansion has typically been detected in peripheral blood genomic DNA by Southern blotting or short tandem repeat polymerase chain reaction (STR-PCR) combined with triplet-repeat primed PCR (TP-PCR) if needed. However both methods estimate the size of the expanded repeat relative to a size standard, and the former requires microgram amounts of DNA. To support the development of therapies, a high-throughput screening approach for repeat expansions in FECD is required. Here, we present a sensitive assay using long-range PCR and nanopore sequencing of genomic DNA to accurately resolve the CTG18.1 repeat.
Methods
The CTG18.1 locus was analysed in genomic DNA from peripheral blood leukocytes by two different methods, and results were compared. The first approach used STR-PCR and capillary electrophoresis, followed by confirmatory testing of apparent homozygotes by TP-PCR. The second used long-range PCR, library preparation and long-read sequencing on an Oxford Nanopore Technologies MinION, with resolution of repeat length using the STRique algorithm.
Results
CTG18.1 expansion was screened for in 119 patients with FECD and 83 controls, by STR/TP-PCR genotyping and, independently, by long-range PCR/long-read nanopore sequencing. Both methods gave comparable results, but the latter was also able to measure repeat length. A total of 73.1% of FECD cases (87/119) and 1.2% of age-matched controls (1/83) had at least one CTG18.1 expansion that was ≥ 50 repeats. The expanded CTG18.1 allele was inherited across multiple generations in four larger families, in a manner consistent with causing a dominant phenotype, revealing that some younger family members may be at risk. The G allele of SNP rs599550, ~1kb away from the expansion, is linked (in cis) with expanded alleles in 80.8% of FECD alleles with an expansion, compared with 12.5% in FECD alleles in cases without an expansion and 14.6% in Europeans.
Discussion
We demonstrate that long-range PCR and long-read nanopore sequencing is a sensitive method requiring only nanograms of DNA, which can be scaled up for high-throughput detection and accurate sizing of CTG18.1 in peripheral blood DNA. The SNP, rs599550, is in linkage disequilibrium with the expansion and physically closer than rs613872, previously used in FECD association studies, making it better for use in diagnostic or association studies
Decay spectroscopy at the two-proton drip line: Radioactivity of the new nuclides 160Os and 156W
No full text2023 Descuento SCOAP
Artículo firmado por 64 autoresThe radioactivity of Os-160(76)84 and W-156(74)82 that lie at the two-proton drip line has been measured in an experiment performed at the Accelerator Laboratory of the University of Jyvaskyla. The Os-160 nuclei were produced using fusion-evaporation reactions induced by a beam of 310 MeV Ni-58 ions bombarding a Cd-106 target. The Os-160 ions were separated in flight using the recoil separator MARA and implanted into a double-sided silicon strip detector, which was used to measure their decays. The.. decays of the ground state of Os-160 (E-alpha = 7092(15) keV, t(1/2) = 97(-32)(+97) mu s) and its isomeric state (E-alpha = 8890(10) keV, t(1/2) = 41(-9)(+15) mu s) were measured, allowing the excitation energy of the isomer to be determined as 1844(18) keV. These alpha-decay properties and the excitation energy of the isomer are compared with systematics. The alpha decays were correlated with subsequent decays to investigate the beta decays of the ground state of W-156, revealing that unlike its isotones, both low-lying isomers were populated in its daughter nuclide, 156Ta. An improved value for the half-life of the proton-decaying high-spin isomeric state in Ta-156(73)83 of 333(-22) (+25) ms was obtained in a separate experiment using the same experimental systems with a Pd-102 target. This result was employed to improve the precision of the half-life determined for W-156, which was measured as 157(-34)(+57) ms.Science and Technology Facilities Council (United Kingdom)European CommissionSlovak Research and Development AgencyVedecka grantova agentura MSVVaS SR a SAVUnited States Department of EnergyResearch Council of FinlandESET Foundation (Slovakia)Depto. de Estructura de la Materia, Física Térmica y ElectrónicaFac. de Ciencias FísicasInstituto de Física de Partículas y del Cosmos (IPARCOS)TRUEpubDescuento UC
Stage, age, and EBV status impact outcomes of plasmablastic lymphoma patients: a clinicopathologic analysis of 61 patients
No full text10.1186/s13045-015-0163-zJournal of Hematology and Oncology816
Decay studies in the A ∼ 225 Po-Fr region from the DESPEC campaign at GSI in 2021
No full textThe HISPEC-DESPEC collaboration aims at investigating the structure of exotic nuclei formed in fragmentation reactions with decay spectroscopy measurements, as part of the FAIR Phase-0 campaign at GSI. This paper reports on first results of an experiment performed in spring 2021, with a focus on β-decay studies in the Po-Fr nuclei in the 220 < A < 230 island of octupole deformation exploiting the DESPEC setup. Ion-beta correlations and fast-timing techniques are being employed, giving an insight into this difficult-to-reach region
Decay spectroscopy at the two-proton drip line: Radioactivity of the new nuclides 160Os and 156W
No full text
