2,249 research outputs found
Luffariella koreana Sim & Lee & Kim 2017, n. sp.
No full text<i>Luffariella koreana</i> n. sp. (Figs. 2, 3) <p> <b>Type specimen.</b> Holotype (NIBRIV0000305502), Munseom, Seogwipo-si, Jeju-do, 5 Sep 2012, Eom TY, by scuba, depth 10 m, deposited in the NIBR.</p> <p> <b>Description.</b> Irregular mass with three tube-like branch on sponge side. Size up to 12 × 4.5 cm and branches, 1-2 cm in diameter. Surface covered with thin membrane. Surface conules indistinct. Several vents open over sponge surface. Color in life dirty gray. Texture firm and compressible.</p> <p>Skeleton: Thin walled tube-like branches consist of dens fibres network with strong collagen. Primary fibres usually uncored, but small part of fibres lightly cored at surface. Near surface, primary fibres arrayed in groups of two or three fibres and connected by secondary fibres in a ladder-like (Fig. 3B). Primary fibres at surface, 80- 350 μm in diameter and 350 μm in diameter at choanosome, irregularly arranged at base of sponge. Secondary fibres, 20-70 μm in diameter. Large meshed regular network, 250-600 μm wide, appeared near surface membrane (Fig. 3A). Primary and secondary fibres network separated from each other. Tertiary fibres, 8-10 μm in diameter, branched out from secondary fibres at choanosome (Fig. 3C).</p> <p> <b>Etymology.</b> This species is named after the type locality, Korea.</p> <p> <b>Remarks.</b> This new species is very similar to <i>Luffariella cylindrica</i> Bergquist 1995 and <i>L. variabilis</i> Polajaeff 1884, partly in its arrangement of skeletal structure, but differs in sponge growth form. Growth form of this new species branches out laterally like repent form, but <i>L. cylindrica</i> and <i>L. variabilis</i> are erect form.</p>Published as part of <i>Sim, Chung Ja, Lee, Kyung Jin & Kim, Young A, 2017, Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea, pp. 190-194 in Journal of Species Research 6 (2)</i> on pages 192-194, DOI: 10.12651/JSR.2017.6.2.190, <a href="http://zenodo.org/record/8120401">http://zenodo.org/record/8120401</a>
Luffariella tubula Sim & Lee & Kim 2017, n. sp.
No full text<i>Luffariella tubula</i> n. sp. (Fig. 1) <p> <b>Type specimen.</b> Holotype (NIBRIV0000305501), Munseom, Seogwipo-si, Jeju-do, 3 Sep 2012, Eom TY, by SCUBA, depth 10 m, deposited in the NIBR.</p> <p> <b>Description.</b> Irregular small mass with tube-like branch repent form, size up to 6.5 × 4 cm. Surface covered with thin membrane, conules indistinct. Thin walled tube, 1-2 mm thick. Several vent holes, 2-5 mm in diameter, open at end of tube. Color in life yellowish beige. Texture very hard and imcompressible. Sponge have a large quantity of collagen with fibres network.</p> <p>Skeleton: The skeleton consists of reticulation of thin-walled. Meshed network of thick primary fibres, separated from secondary and tertiary fibres (Fig. 1C). Primary fibres usually uncored from debris, but cored fibres appeared near surface membrane rarely. Primary fibres usually irregular shape and variable in diameter, but cored fibres, 150 μm in diameter, near surface. Uncored primary fibres, 80-200 μm in diameter, at choanosome (Fig. 1H), Two sizes of regular secondary fibres, 60-100 μm and 40-60 μm in diameter. Tertiary fibres, 10-15 μm in diameter (Fig. 1C).</p> <p> <b>Etymology.</b> This species is named after its tube-like shape.</p> <p> <b>Remarks.</b> This new species is very similar to the genus <i>Hyattella</i> in the skeletal structure of secondary fibres, but differs in lacking cored primary fibres. Thickly branched primary and tertiary fibres appeared in choanosome.</p>Published as part of <i>Sim, Chung Ja, Lee, Kyung Jin & Kim, Young A, 2017, Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea, pp. 190-194 in Journal of Species Research 6 (2)</i> on pages 190-192, DOI: 10.12651/JSR.2017.6.2.190, <a href="http://zenodo.org/record/8120401">http://zenodo.org/record/8120401</a>
SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus‐oocyte complex penetration defect
No full textThe glycosylphosphatidylinositol-anchored sperm hyaluronidases (Hyals), sperm adhesion molecule 1 (SPAM1) and HYAL5, have long been believed to assist in sperm penetration through the cumulus-oocyte complex (COC), but their role in mammalian fertilization remains unclear. Previously, we have shown that mouse sperm devoid of either Spam1 or Hyal5 are still capable of penetrating the COC and that the loss of either Spam1 or Hyal5 alone does not cause male infertility in mice. In the present study, we found that Spam1/Hyal5 double knockout (dKO) mice produced significantly fewer offspring compared with wild-type (WT) mice, and this was due to defective COC dispersal. A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the COC. This impaired sperm activity is likely due to the deficiency in the sperm Hyals, even though other somatic Hyals are expressed normally in the dKO mice. The fertilization ability of the Spam1/Hyal5 dKO sperm was restored by adding purified human sperm Hyal to the in vitro fertilization medium. Our results suggest that Hyal deficiency in sperm may be a significant risk factor for male sterility.-Park, S., Kim, Y.-H., Jeong, P.-S., Park, C., Lee, J.-W., Kim, J.-S., Wee, G., Song, B.-S., Park, B.-J., Kim, S.-H., Sim, B.-W., Kim, S.-U., Triggs-Raine, B., Baba, T., Lee, S.-R., Kim, E. SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
Iris rossii var. latifolia J. K. Sim & Y. S. Kim 1992
No full textIris rossii var. latifolia J.K. Sim & Y.S. Kim (1992: 1) = I. rossii f. latifolia (J.K. Sim & Y.S. Kim) M. Kim (2017: 570) Holotype: KOREA, Jeollabuk-do: Chongup-gun, Ibam-myeon, Noryeong, 7 May 1987, J. K. Sim 87001 (NIBRVP815290). Isotypes: KOREA, Jeollabuk-do: Chongup-gun, Ibam-myeon, Noryeong, 7 May 1987, J. K. Sim 87003 (NIBRVP815291); 87004 (NIBRVP815288); 87005 (NIBRVP815289; Fig. 1 -36). Paratypes: KOREA, Daejeon-si: Mt. Bomunsan, 19 May 1990, J. K . Sim s.n. (4 sheets, NIBRVP815292, NIBRVP815293, NIBRVP815294, NIBRVP815295); Mt. Sikjangsan, 21 April 1990, J. K . Sim s.n. (4 sheets, NIBRVP815299, NIBRVP815300, NIBRVP815301, NIBRVP815302). Chungcheongnam-do: Gongju-si, Banpo-myeon, Oncheon-ri, Mt. Gapasan, 30 April 1992, Y. I . Kim 70 (NIBRVP815296); Gongju-si, Gyeryong-myeon, Mt. Gyeryongsan, 4 May 1987, J. K . Sim 27 (NIBRVP815297). Jeollanam-do: Mt. Daedunsan, 8 May 1987, B. J . Yu s.n. (NIBRVP815298). Note: These specimens were transferred to KB from the herbarium of Mokwon University. Some of the paratype specimens donated by Sim & Kim (1992) were not found in KB.Published as part of Jang, Hyun-Do, Hyun, Chang-Woo, Ryu, Seah & Lee, Sang-Jun, 2022, Type specimens of vascular plants in the herbarium of the National Institute of Biological Resources (II), pp. 229-243 in Phytotaxa 539 (3) on page 241, DOI: 10.11646/phytotaxa.539.3.2, http://zenodo.org/record/636408
Kinetic analysis of precursor M1 RNA molecules for exploring substrate specificity of the N-terminal catalytic half of RNase E
No full textTo gain insight into the mechanism by which the sequence at the rne-dependent site of substrate RNA affects the substrate specificity of Escherichia coli RNase E, we performed kinetic analysis of the cleavage of precursor M1 RNA molecules containing various sequences at the rne-dependent site by the N-terminal catalytic half of RNase E (NTH-RNase E). NTH-RNase E displayed higher K-m and k(cat) values for more specific substrates. The retention of single strandedness at the rne-dependent site was essential for cleavage efficiency. Moreover, the loss of single-strandedness was accompanied by a decrease in both the K-m and k(eat) values
Current-Mode Transceiver for Silicon Interposer Channel
No full textAn energy-efficient 3 Gb/s current-mode interface scheme is proposed for on-chip global interconnects and silicon interposer channels. The transceiver core consists of an open-drain transmitter with one-tap pre-emphasis and a current sense amplifier load as the receiver. The current sense amplifier load is formed by stacking a PMOS diode stage and a cross-coupled NMOS stage, providing an optimum current-mode receiver without any bias current. The proposed scheme is verified with two cases of transceivers implemented in 65 nm CMOS. A 10 mm point-to-point data-only channel shows an energy efficiency of 9.5 fJ/b/mm, and a 20 mm four-drop source-synchronous link achieves 29.4 fJ/b/mm including clock and data channels.X111518sciescopu
Fig. 2. Luffariella koreana n in Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea
No full textFig. 2. Luffariella koreana n. sp. A, entire animal; B, skeletal structure; C, surface primary fibre; D, closed skeletal structure; E, primary fibres surrounded secondary and tertiary fibres; F, secondary and primary fibres separated with each other (arrow). Scale bars: A = 3 cm, B = 50 μm C-E = 100 μm, F = 50 μm.Published as part of Sim, Chung Ja, Lee, Kyung Jin & Kim, Young A, 2017, Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea, pp. 190-194 in Journal of Species Research 6 (2) on page 192, DOI: 10.12651/JSR.2017.6.2.190, http://zenodo.org/record/812040
Fig. 1. Luffariella tubula n in Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea
No full textFig. 1. Luffariella tubula n. sp. A, entire animal; B, closed surface; C, primary fibres separate from secondary and tertiary fibres; D, cored primary fibres near surface; E, F, choanosome skeletal structure. G, skeletal structure with collagen; H, thick branched primary fibres with secondary and tertiary fibres. Scale bars: A = 2 cm, B = 1 cm, C, D = 200 μm, E-H = 100 μm.Published as part of Sim, Chung Ja, Lee, Kyung Jin & Kim, Young A, 2017, Two new species of genus Luffariella (Dictyoceratida: Thorectidae) from Korea, pp. 190-194 in Journal of Species Research 6 (2) on page 191, DOI: 10.12651/JSR.2017.6.2.190, http://zenodo.org/record/812040
A Systematic Approach for the Determination of B-Group Vitamins in Multivitamin Dietary Supplements by High Performance Liquid Chromatography with Diode-Array Detection and Mass Spectrometry
No full textThis paper describes a practical approach using an LC diode-array detector (DAD) and MS for the quantitation of B-group vitamins (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine, biotin, folic acid, and cyanocobalamin) in multivitamin supplements. The purpose of this study was to optimize the extraction and chromatographic conditions without application of internal standards to determine B-group vitamins in multivitamins. Chromatographic separation on a C18 column was optimized on the basis of chromatographic behavior depending on pH variation of the mobile phase. The optimized methods showed good r values, with RSD values lower than 2.44 and 3.09% for most of the B-group vitamins (except cyanocobalamin) using LC DAD and LC-MS, respectively. The methods were also proved to be reproducible, with RSD values of 0.96 and 2.48% being obtained with LC DAD and LC-MS, respectively. In addition, the developed analytical methods for B-group vitamins were validated using certified reference materials and were applied to commercially available multivitamin tablet
All-Synthesizable Current-Mode Transmitter Driver for USB2.0 interface
No full textAn all-synthesizable current-mode transmitter driver for a USB2.0 high-speed (480 Mb/s) interface was proposed to enhance the design portability. The proposed driver was implemented using tristate inverter cells. It uses the differential current-mode architecture, with variable output voltage swing, and includes a predriver. It was also successfully applied to the 480-Mb/s USB2.0 TX driver with the synthesized serializer and phase-locked loop.111sciescopu
- …
