1,720,962 research outputs found
Complete genome sequences of Blautia hydrogenotrophica DSM 10507 T isolated from human feces and Blautia coccoides DSM 935 T isolated from mouse feces
No full textABSTRACT
We report on the closed genome sequences of the acetogen
Blautia hydrogenotrophica
S5a33
T
(DSM 10507
T
) and of
Blautia coccoides
CLC-1
T
(DSM 935
T
). The
B. hydrogenotrophica
S5a33
T
genome harbors a chromosome (3,590,609 bp) and a plasmid (7,176 bp). The
B. coccoides
CLC-1
T
genome consists of a single chromosome (6,097,890 bp).Open-Access-Publikationsfonds 202
First Insights Into Bacterial Gastrointestinal Tract Communities of the Eurasian Beaver (Castor fiber)
No full textThe Eurasian or European beaver (Castor fiber) is the second-largest living rodent after the capybara. It is a semi-aquatic animal known for building dams and lodges. They strictly feed on lignocellulose-rich plants and correspondingly harbor cellulolytic microbial communities in their digestive tract. In this study, the bacterial community composition, diversity, and functional profile of different gut compartments ranging from stomach to colon have been explored. A total of 277 bacterial operational taxonomic units (OTUs) at species level were obtained from the gut systems of two males (juvenile and subadult) and one subadult female beaver. In general, cecum and colon are dominated by Firmicutes and Actinobacteria. High abundance of Bacteroidetes was observed only in male juvenile beaver cecum and colon, suggesting that the bacterial composition changes with age. Within the cecum and colon, members of known cellulase-producing bacterial taxa including the families Ruminococcaceae, Lachnospiraceae, and Clostridiaceae 1 were detected. The presence of putative genes encoding cellulolytic and carbohydrate-degrading enzymes indicated also the degradation of recalcitrant plant material in both gut compartments. The bacterial community in the gut systems of the Eurasian beaver differed from that of the North American beaver. Higher abundance of Actinobacteria and lower abundances of Bacteroidetes were recorded in the Eurasian beaver. Similar differences were obtained to bacterial communities of termites and herbivorous animals such as bovine. The data presented in this study provides the first insight into bacterial communities in the gut system of the Eurasian beave
Draft Genome Sequence of the Hydrogenogenic Carboxydotroph Moorella stamsii DSM 26271
No full textABSTRACT
The spore-forming, thermophilic, and obligate anaerobic bacterium
Moorella stamsii
was isolated from digester sludge. Apart from its ability to use carbon monoxide for growth,
M. stamsii
harbors several enzymes for the use of different sugars. The draft genome has a size of 3,329 Mb and contains 3,306 predicted protein-encoding genes.
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Complete genome sequence of Sporomusa sphaeroides DSM 2875 T isolated from mud of the Leine river and Sporomusa ovata DSM 2662 T isolated from sugar beet leaf silage
No full textABSTRACT We report the closed genome sequences of the acetogen Sporomusa sphaeroides E T (DSM 2875 T ) and of Sporomusa ovata H1 T (DSM 2662 T ). The S. sphaeroides E T genome harbors a chromosome (4,956,256 bp) and a plasmid (59,087 bp). The genome of S. ovata H1 T harbors one chromosome (5,433,971 bp).Open-Access-Publikationsfonds 202
Isolation and characterization of Sporomusa carbonis sp. nov.: a carboxydotrophic hydrogenogen in the genus of Sporomusa isolated from a charcoal-burning pile
No full textA Gram-negative bacterial strain, designated ACPt T , was isolated from the top of the covering soil of an active charcoal-burning pile. The cells of ACPt T were strictly anaerobic, rod-shaped and grew optimally at 40 °C and pH 7. The substrates ribose, glucose, sucrose, raffinose, melezitose, pyruvate, vanillate, syringate, methanol and CO were utilized for growth. Phylogenomic analysis of the 4.1 Mb genome showed that strain ACPt T represented a novel species of the genus Sporomusa . The most closely related species to ACPt T was Sporomusa malonica, with an average amino acid identity of 80.1%. The genome of ACPt T encoded cytochromes, ubiquinones, the Wood–Ljungdahl gene cluster and an Rnf complex, which were identified as common features of all Sporomusa type strains. However, strain ACPt T did not ferment H 2 +CO 2 via acetogenesis as other Sporomusa species but employed the metabolism of a carboxydotrophic hydrogenogen, converting CO to H 2 +CO 2 . Based on the genomic, morphological and physiological features presented in this study, strain ACPt T is proposed as a novel species in the genus Sporomusa, with the name Sporomusa carbonis sp. nov. (DSM 116159 T and CCOS 2105 T )
Genome-based metabolic and phylogenomic analysis of three Terrisporobacter species.
No full textAcetogenic bacteria are of high interest for biotechnological applications as industrial platform organisms, however, acetogenic strains from the genus Terrisporobacter have hitherto been neglected. To date, three published type strains of the genus Terrisporobacter are only covered by draft genome sequences, and the genes and pathway responsible for acetogenesis have not been analyzed. Here, we report complete genome sequences of the bacterial type strains Terrisporobacter petrolearius JCM 19845T, Terrisporobacter mayombei DSM 6539T and Terrisporobacter glycolicus DSM 1288T. Functional annotation, KEGG pathway module reconstructions and screening for virulence factors were performed. Various species-specific vitamin, cofactor and amino acid auxotrophies were identified and a model for acetogenesis of Terrisporobacter was constructed. The complete genomes harbored a gene cluster for the reductive proline-dependent branch of the Stickland reaction located on an approximately 21 kb plasmid, which is exclusively found in the Terrisporobacter genus. Phylogenomic analysis of available Terrisporobacter genomes suggested a reclassification of most isolates as T. glycolicus into T. petrolearius
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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