1 research outputs found
Characterization of the Solanum tuberosum-derived Sli gene
No full textThe Sli gene, discovered in a wild diploid species Solanum chacoense, converts self-incompatible diploid potatoes to self-compatible ones. Previously, the presence of Sli was predicted in S. tuberosum varieties. In this study, we developed a marker system to identify Sli and revealed that 76 out of 152 varieties and breeding clones possess Sli. Dihaploid clones were generated from 4x breeding clones carrying Sli. Self-crossing was conducted for 12 2xSli-carriers, one of which was self-fertile (SF). The progeny of the SF clone segregated into seven Sli homozygotes and eight Sli heterozygotes, fitting a 1:1 segregation ratio and supporting the gametophytic action of Sli. Through random crossings among the dihaploid and S1 plants, 60 plants were obtained and characterized. Of the 51 Sli-carrying plants, 37 were self-pollinated on more than ten flowers, resulting in three SF plants (8.1%). Pollen tube growth among Sli carriers varied, regardless of whether they were SF or not. Both S. chacoense-derived Sli (Slichc) and S. tuberosum-derived Sli (Slitbr) were expressed in the pollen. The obtained 2702-bp sequence, containing the Sli gene (1810?bp) and a 533-bp insertion in the promoter region, was identical in Slitbr and Slichc. While both may function similarly in selfing, producing SF plants through outcrossing from Slitbr carriers is less efficient, indicating that other factors may influence self-fertility. c 2025 Elsevier B.V., All rights reserved
